scholarly journals Functional, structural, and spectroscopic characterization of a glutathione-ligated [2Fe–2S] cluster in poplar glutaredoxin C1

2007 ◽  
Vol 104 (18) ◽  
pp. 7379-7384 ◽  
Author(s):  
Nicolas Rouhier ◽  
Hideaki Unno ◽  
Sibali Bandyopadhyay ◽  
Lluis Masip ◽  
Sung-Kun Kim ◽  
...  
Keyword(s):  
Intracellular Localization ◽  
Spectroscopic Characterization ◽  
Spectroscopic Studies ◽  
Iron Sulfur Cluster ◽  
X Ray Crystallography ◽  
Iron Sulfur ◽  
Catalytic Cysteine ◽  
A Subunit ◽  
Stress Sensing

When expressed in Escherichia coli, cytosolic poplar glutaredoxin C1 (CGYC active site) exists as a dimeric iron–sulfur-containing holoprotein or as a monomeric apoprotein in solution. Analytical and spectroscopic studies of wild-type protein and site-directed variants and structural characterization of the holoprotein by using x-ray crystallography indicate that the holoprotein contains a subunit-bridging [2Fe–2S] cluster that is ligated by the catalytic cysteines of two glutaredoxins and the cysteines of two glutathiones. Mutagenesis data on a variety of poplar glutaredoxins suggest that the incorporation of an iron–sulfur cluster could be a general feature of plant glutaredoxins possessing a glycine adjacent to the catalytic cysteine. In light of these results, the possible involvement of plant glutaredoxins in oxidative stress sensing or iron–sulfur biosynthesis is discussed with respect to their intracellular localization.

scholarly journals Spectroscopic characterization of the novel iron-sulfur cluster in Pyrococcus furiosus ferredoxin.

1990 ◽  
Vol 265 (15) ◽  
pp. 8533-8541
Author(s):  
R C Conover ◽  
A T Kowal ◽  
W G Fu ◽  
J B Park ◽  
S Aono ◽  
...  
Keyword(s):  
Pyrococcus Furiosus ◽  
Spectroscopic Characterization ◽  
The Novel ◽  
Iron Sulfur Cluster ◽  
Sulfur Cluster ◽  
Iron Sulfur

scholarly journals Characterizing SPASM/twitch Domain-Containing Radical SAM Enzymes by EPR Spectroscopy

2021 ◽  
Author(s):  
Aidin R. Balo ◽  
Lizhi Tao ◽  
R. David Britt
Keyword(s):  
Epr Spectroscopy ◽  
Continuous Wave ◽  
Spectroscopic Studies ◽  
Paramagnetic Species ◽  
Paramagnetic Resonance ◽  
Iron Sulfur Cluster ◽  
Radical Sam Enzymes ◽  
Iron Sulfur ◽  
Pulse Epr ◽  
Multiple Domains

AbstractOwing to their importance, diversity and abundance of generated paramagnetic species, radical S-adenosylmethionine (rSAM) enzymes have become popular targets for electron paramagnetic resonance (EPR) spectroscopic studies. In contrast to prototypic single-domain and thus single-[4Fe–4S]-containing rSAM enzymes, there is a large subfamily of rSAM enzymes with multiple domains and one or two additional iron–sulfur cluster(s) called the SPASM/twitch domain-containing rSAM enzymes. EPR spectroscopy is a powerful tool that allows for the observation of the iron–sulfur clusters as well as potentially trappable paramagnetic reaction intermediates. Here, we review continuous-wave and pulse EPR spectroscopic studies of SPASM/twitch domain-containing rSAM enzymes. Among these enzymes, we will review in greater depth four well-studied enzymes, BtrN, MoaA, PqqE, and SuiB. Towards establishing a functional consensus of the additional architecture in these enzymes, we describe the commonalities between these enzymes as observed by EPR spectroscopy.

Phosphoramides bearing isoxazole derivative: spectroscopic and structural characterization, study of hydrogen-bonding interactions and two lanthanide complexes (LnIII= Ce and Eu)

2015 ◽  
Vol 71 (2) ◽  
pp. 176-185 ◽  
Author(s):  
Khodayar Gholivand ◽  
Foroogh Molaei ◽  
Mahdieh Hosseini
Keyword(s):  
Lanthanide Complexes ◽  
Density Functional ◽  
Spectroscopic Characterization ◽  
Crystalline Lattice ◽  
Ligand Interaction ◽  
Functional Theory ◽  
X Ray Crystallography ◽  
Isoxazole Derivative ◽  
Characterization Study

In this study, the synthesis and spectroscopic characterization of new phosphoramides based on 3-amino-5-methylisoxazole with the formulaR2P(O)[NH–C4H4NO],R= C6H5O (1), C6H5(2),RP(O)[NH—C4H4NO]2,R= C6H5O (3), CH3—C6H4O (4), C6H5NH (5), (C6H5)ClP(O)[NH–C4H4NO] (6) and two lanthanide complexes [Ln(2)2(NO3)3(EtOH)]·EtOH, LnIII= Ce (7) and Eu (8), have been reported. The structural study of (3) shows the presence of two conformers (crystallographically independent molecules) in the crystalline lattice, caused by different orientations of the phenyl and isoxazole rings. For (3), the intermolecular interactions have been studied by Hirshfeld surface analysis and fingerprint plots. Furthermore, the electronic and energy aspects of hydrogen bonds between molecules of (3) have been explored by density functional theory (DFT) calculations. X-ray crystallography of complexes (7) and (8) reveals that two phosphoramide ligands take part in coordination to the metal, one as monodentate from Ophosphoryl, and the other one as chelate through Ophosphoryland Nring. The complexes are also composed of two conformers in the solid-state structure. Quantum theory of atoms in molecules (QTAIM) analysis discloses the electrostatic nature of the Ln–ligand interaction.

A Vanadium-Containing Iron−Sulfur Cluster with a V2Fe2S4Cubane-like Core. Synthesis, Structure, and Characterization of (Et4N)[V2Fe2S4(Me2dtc)5]

1997 ◽  
Vol 36 (2) ◽  
pp. 214-219 ◽  
Author(s):  
Yuheng Deng ◽  
Qiutian Liu ◽  
Yu Yang ◽  
Youtong Wang ◽  
Yuanba Cai ◽  
...  
Keyword(s):  
Iron Sulfur Cluster ◽  
Sulfur Cluster ◽  
Iron Sulfur

Synthesis and structural characterization of a new chromium-iron-sulfur cluster, (.eta.5-C5H5)2Cr2Fe2(.mu.3-CO)2(.mu.3-S)2(CO)6

1981 ◽  
Vol 20 (11) ◽  
pp. 3586-3589 ◽  
Author(s):  
Pierre. Braunstein ◽  
Antonio. Tiripicchio ◽  
Marisa. Tiripicchio Camellini ◽  
Enrico. Sappa
Keyword(s):  
Structural Characterization ◽  
Iron Sulfur Cluster ◽  
Sulfur Cluster ◽  
Iron Sulfur

Synthesis, X-ray crystallography, thermogravimetric analysis and spectroscopic characterization of isostructural one-dimensional coordination polymers as sorbents for some anions

CrystEngComm ◽  
2018 ◽  
Vol 20 (13) ◽  
pp. 1783-1796 ◽  
Author(s):  
Bahram Ghanbari ◽  
Leila Shahhoseini ◽  
Agata Owczarzak ◽  
Maciej Kubicki ◽  
Reza Kia ◽  
...  
Keyword(s):  
Thermogravimetric Analysis ◽  
Coordination Polymers ◽  
Spectroscopic Characterization ◽  
One Dimensional ◽  
X Ray ◽  
X Ray Crystallography

A new series of coordination polymers capable of adsorbing anions have been synthesized by employing a dipyridine substituted diazacrown macrocycle as linker.

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