scholarly journals Chimeras of Dictyostelium myosin II head and neck domains with Acanthamoeba or chicken smooth muscle myosin II tail domain have greatly increased and unregulated actin-dependent MgATPase activity

2000 ◽  
Vol 97 (23) ◽  
pp. 12553-12558 ◽  
Author(s):  
X. Liu ◽  
S. Shu ◽  
R. A. Yamashita ◽  
Y. Xu ◽  
E. D. Korn
Keyword(s):  
Smooth Muscle ◽  
Head And Neck ◽  
Myosin Ii ◽  
Smooth Muscle Myosin ◽  
Tail Domain ◽  
Dictyostelium Myosin ◽  
Muscle Myosin ◽  
Mgatpase Activity

scholarly journals Using the SpyTag SpyCatcher system to label smooth muscle myosin II filaments with a quantum dot on the regulatory light chain

Cytoskeleton ◽  
2019 ◽  
Vol 76 (2) ◽  
pp. 192-199 ◽  
Author(s):  
Richard K. Brizendine ◽  
Murali Anuganti ◽  
Christine R. Cremo
Keyword(s):  
Smooth Muscle ◽  
Quantum Dot ◽  
Light Chain ◽  
Myosin Ii ◽  
Regulatory Light Chain ◽  
Smooth Muscle Myosin ◽  
Muscle Myosin

scholarly journals Regulation of Asymmetric Smooth Muscle Myosin II Molecules

2000 ◽  
Vol 275 (52) ◽  
pp. 41273-41277 ◽  
Author(s):  
H. Lee Sweeney ◽  
Li-Qiong Chen ◽  
Kathleen M. Trybus
Keyword(s):  
Smooth Muscle ◽  
Myosin Ii ◽  
Smooth Muscle Myosin ◽  
Muscle Myosin

scholarly journals 3D Reconstruction of the Folded, Inhibited Form of Vertebrate Smooth Muscle Myosin II by Single Particle Analysis

2017 ◽  
Vol 112 (3) ◽  
pp. 266a ◽  
Author(s):  
Shixin Yang ◽  
Kyounghwan Lee ◽  
Osamu Sato ◽  
Mitsuo Ikebe ◽  
Roger Craig
Keyword(s):  
Smooth Muscle ◽  
3D Reconstruction ◽  
Single Particle ◽  
Myosin Ii ◽  
Smooth Muscle Myosin ◽  
Particle Analysis ◽  
Single Particle Analysis ◽  
Muscle Myosin

scholarly journals The N-terminal Lobes of Both Regulatory Light Chains Interact with the Tail Domain in the 10 S-inhibited Conformation of Smooth Muscle Myosin

2006 ◽  
Vol 281 (50) ◽  
pp. 38801-38811 ◽  
Author(s):  
Bridget Salzameda ◽  
Kevin C. Facemyer ◽  
Brian W. Beck ◽  
Christine R. Cremo
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Myosin ◽  
Light Chains ◽  
Tail Domain ◽  
Regulatory Light Chains ◽  
Muscle Myosin

Blebbistatin inhibits the chemotaxis of vascular smooth muscle cells by disrupting the myosin II-actin interaction

2008 ◽  
Vol 294 (5) ◽  
pp. H2060-H2068 ◽  
Author(s):  
Hong Hui Wang ◽  
Hideyuki Tanaka ◽  
Xiaoran Qin ◽  
Tiejun Zhao ◽  
Li-Hong Ye ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Myosin Ii ◽  
Muscle Cells ◽  
Smooth Muscle Myosin ◽  
A7r5 Cells ◽  
Microscopic Evaluation ◽  
Muscle Myosin

Blebbistatin is a myosin II-specific inhibitor. However, the mechanism and tissue specificity of the drug are not well understood. Blebbistatin blocked the chemotaxis of vascular smooth muscle cells (VSMCs) toward sphingosylphosphorylcholine (IC50 = 26.1 ± 0.2 and 27.5 ± 0.5 μM for GbaSM-4 and A7r5 cells, respectively) and platelet-derived growth factor BB (IC50 = 32.3 ± 0.9 and 31.6 ± 1.3 μM for GbaSM-4 and A7r5 cells, respectively) at similar concentrations. Immunofluorescence and fluorescent resonance energy transfer analysis indicated a blebbistatin-induced disruption of the actin-myosin interaction in VSMCs. Subsequent experiments indicated that blebbistatin inhibited the Mg2+-ATPase activity of the unphosphorylated (IC50 = 12.6 ± 1.6 and 4.3 ± 0.5 μM for gizzard and bovine stomach, respectively) and phosphorylated (IC50 = 15.0 ± 0.6 μM for gizzard) forms of purified smooth muscle myosin II, suggesting a direct effect on myosin II motor activity. It was further observed that the Mg2+-ATPase activities of gizzard myosin II fragments, heavy meromyosin (IC50 = 14.4 ± 1.6 μM) and subfragment 1 (IC50 = 5.5 ± 0.4 μM), were also inhibited by blebbistatin. Assay by in vitro motility indicated that the inhibitory effect of blebbistatin was reversible. Electron-microscopic evaluation showed that blebbistatin induced a distinct conformational change (i.e., swelling) of the myosin II head. The results suggest that the site of blebbistatin action is within the S1 portion of smooth muscle myosin II.

Phosphorylation on Threonine-18 of the Regulatory Light Chain Dissociates the ATPase and Motor Properties of Smooth Muscle Myosin II

Biochemistry ◽  
1995 ◽  
Vol 34 (39) ◽  
pp. 12576-12583 ◽  
Author(s):  
Anne R. Bresnick ◽  
Vicki L. Wolff-Long ◽  
Otto Baumann ◽  
Thomas D. Pollard
Keyword(s):  
Smooth Muscle ◽  
Light Chain ◽  
Myosin Ii ◽  
Regulatory Light Chain ◽  
Smooth Muscle Myosin ◽  
Muscle Myosin

Smooth-Muscle Myosin II

2007 ◽  
pp. 171-222 ◽  
Author(s):  
Christine R. Cremo ◽  
David J. Hartshorne
Keyword(s):  
Smooth Muscle ◽  
Myosin Ii ◽  
Smooth Muscle Myosin ◽  
Muscle Myosin

scholarly journals Myosin light chain kinase steady-state kinetics: comparison of smooth muscle myosin II and nonmuscle myosin IIB as substrates

2016 ◽  
Vol 34 (7) ◽  
pp. 469-474 ◽  
Author(s):  
Diego B. Alcala ◽  
Brian D. Haldeman ◽  
Richard K. Brizendine ◽  
Agata K. Krenc ◽  
Josh E. Baker ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Steady State ◽  
Light Chain ◽  
Myosin Light Chain Kinase ◽  
Myosin Light Chain ◽  
Myosin Ii ◽  
Smooth Muscle Myosin ◽  
Nonmuscle Myosin ◽  
Steady State Kinetics ◽  
Muscle Myosin
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