Trans-Species Rescue of Defective Genomes of Murine Sarcoma Virus from Hamster Tumor Cells with Helper Feline Leukemia Virus

Fujinaga et al reported MSV induced rat and hamster osteosarcoma which showed an occassional unusual bud in the rat induced tumors. Savage and Hackett and Hackett and Sylvester reported abnormal type C virus in UCLB cells derived from Balb/3t3 cells infected and transformed with MLV. They wer unable to demonstrate sarcoma virus activity. Fischinger and O‘Connor reported the infection of cat embryo cells by a centrifugally induced aggregate of murine sarcoma virus and feline leukemia virus designated as MSV(FelLV). This virus gave rise to a defective, focus forming virus which propagated in cat cells but not in mouse cells.In the present study the morphoiogy of the MSV(FelLV) virus obtained from Dr. Fischinger and maintained in our laboratory since 1970 will be reported. Feline embryo fibroblasts (established in our lab.) and Crandall feline kidney cells (Cutter-Haver-Lockhart, Shawnee, Kansas) were used in this study.

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Brief Communication: Aberrant Viruses in Cells Infected With Murine Sarcoma Virus-Feline Leukemia Virus2

JNCI Journal of the National Cancer Institute ◽

10.1093/jnci/54.3.763 ◽

1975 ◽

Keyword(s):

Leukemia Virus ◽

Feline Leukemia Virus ◽

Murine Sarcoma Virus ◽

Sarcoma Virus ◽

Murine Sarcoma ◽

In Cells

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Viral gene expression in murine sarcoma virus(murine leukemia virus)-infected cells.

Journal of Virology ◽

10.1128/jvi.27.3.768-775.1978 ◽

1978 ◽

Vol 27 (3) ◽

pp. 768-775 ◽

Cited By ~ 8

Author(s):

D Dina ◽

E E Penhoet

Keyword(s):

Gene Expression ◽

Murine Leukemia Virus ◽

Leukemia Virus ◽

Viral Gene Expression ◽

Viral Gene ◽

Murine Sarcoma Virus ◽

Sarcoma Virus ◽

Infected Cells ◽

Murine Sarcoma ◽

Murine Leukemia

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ANTIGENIC PROPERTIES OF MURINE SARCOMA VIRUS-TRANSFORMED BALB/3T3 NONPRODUCER CELLS

Journal of Experimental Medicine ◽

10.1084/jem.135.3.503 ◽

1972 ◽

Vol 135 (3) ◽

pp. 503-515 ◽

Cited By ~ 61

Author(s):

John R. Stephenson ◽

Stuart A. Aaronson

Keyword(s):

Cell Line ◽

Leukemia Virus ◽

Target Cells ◽

Subsequent Challenge ◽

Murine Sarcoma Virus ◽

Sarcoma Virus ◽

Rauscher Leukemia ◽

Transplantation Antigens ◽

Murine Sarcoma ◽

Rauscher Leukemia Virus

The isolation of clonal lines of murine sarcoma virus-transformed, non-producer BALB/3T3 cells has provided a model system for determining whether RNA tumor virus-transformed cells possess virus-specific transplantation antigens. MSV nonproducer cells (K-234) were clonally derived from an inbred mouse cell line, BALB/3T3. A parallel virus-producing cell line was obtained by infection of the MSV nonproducer cells with Rauscher leukemia virus. K-234 was much more tumorigenic than K-234(R). Preimmunization of syngeneic mice with either K-234(R) or with UV-inactivated Rauscher leukemia virus induced transplantation resistance to subsequent challenge with K-234(R), but not with K-234. In contrast, mice preimmunized with nonproducer cells were not made resistant to subsequent challenge with the homologous cells. Antisera prepared from mice immunized with K-234(R) were specifically cytotoxic and positive by fluorescent antibody staining for K-234(R) target cells, but not to either BALB/3T3 or K-234. The results show that MSV nonproducer cells lack detectable transplantation antigens and suggest that the transplantation resistance to the producing cells is attributable to maturing virus at the cell surface.

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Kirsten murine sarcoma virus abolishes interferon gamma-induced class II but not class I major histocompatibility antigen expression in a murine fibroblast line.

Journal of Experimental Medicine ◽

10.1084/jem.167.2.706 ◽

1988 ◽

Vol 167 (2) ◽

pp. 706-711 ◽

Cited By ~ 9

Author(s):

D J Maudsley ◽

A G Morris

Keyword(s):

Leukemia Virus ◽

Constitutive Expression ◽

Antigen Expression ◽

Class Ii ◽

Class I ◽

Ras Gene ◽

Ifn Gamma ◽

Murine Sarcoma Virus ◽

Sarcoma Virus ◽

Murine Sarcoma

The effect of infecting fibroblasts with Kirsten murine sarcoma virus/murine leukemia virus (Ki-MSV/MLV) on constitutive and IFN-gamma-induced H-2 antigen expression was investigated. The fibroblasts used were two established cell lines (C3H10T1/2 and BALB/c3T3) and fresh embryo fibroblasts from C3H mice. Class I antigens were expressed constitutively by BALB/c3T3; infection with MLV, MSV or the two together had little effect on this constitutive expression. Class I antigens (H-2K, H-2D) were strongly induced on all three types of fibroblast by rIFN-gamma, and infection had little effect on this. None of the fibroblasts expressed constitutively detectable levels of class II antigen; however, C3H10T1/2 fibroblasts could be induced for both H-2A and H-2E by IFN-gamma. Infection of C3H10T1/2 with helper-free Ki-MSV, or MSV together with MLV, completely abolished this induction of class II antigens, while infection with MLV alone had little effect, implying that the abolition of class II induction was due to genomic regions of Ki-MSV not shared with Ki-MLV, probably the v-Ki-ras gene.

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