scholarly journals Freeze-fracture study of mast cell secretion.

1976 ◽  
Vol 73 (8) ◽  
pp. 2823-2827 ◽  
Author(s):  
E. Y. Chi ◽  
D. Lagunoff ◽  
J. K. Koehler
Keyword(s):  
Mast Cell ◽  
Freeze Fracture ◽  
Cell Secretion ◽  
Fracture Study

scholarly journals A freeze-fracture study of early membrane events during mast cell secretion.

1977 ◽  
Vol 73 (3) ◽  
pp. 660-671 ◽  
Author(s):  
S J Burwen ◽  
B H Satir
Keyword(s):  
Mast Cell ◽  
Plasma Membrane ◽  
Membrane Fusion ◽  
Ionic Composition ◽  
Freeze Fracture ◽  
Polymyxin B ◽  
Cell Secretion ◽  
Granule Membrane ◽  
Fracture Study ◽  
Freeze Fracture Electron Microscopy

The early membrane events taking place during mast cell secretion were followed in transmission and freeze-fracture electron microscopy. In order to slow down exocytosis and capture intermediate stages of membrane fusion, special conditions of incubation and stimulation were used. These were as follows: (a) the use of incubation media with altered ionic composition, and (b) stimulation with a low dosage of polymyxin B sulfate (4 microgram/ml) at low temperature (18 degrees C) for very short incubation times (30-60 s), with or without the presence of formaldehyde (0.8%). Under these conditions, unetchable circular impressions are found on the E face of the plasma membrane, 80-100 nm in diameter, with particles associated with their perimeters. In granule-to-granule fusion, the zone involved is demarcated by one or two rows of particles on the E face. In addition, raised circular areas of varying diameters (43-87 nm) surrounded by similar particles, also found on the E face, may represent potential sites before completion of fusion. Neither the circular impressions on the plasma membrane nor the sites on the granule membrane are permanent, but their appearance coincides with initiation of membrane fusion.

Junctional Pattern in the Squamous Metaplasia of the Female Trigone. A Freeze-Fracture Study

1983 ◽  
Vol 129 (2) ◽  
pp. 280-283 ◽  
Author(s):  
M. Sideri ◽  
G. De Virgiliis ◽  
R. Rainoldi ◽  
A. Ferrari ◽  
G. Remotti
Keyword(s):  
Squamous Metaplasia ◽  
Freeze Fracture ◽  
Fracture Study

Freeze-fracture study of the mechanoreceptive digital corpuscles of mice

1985 ◽  
Vol 14 (6) ◽  
pp. 1037-1052 ◽  
Author(s):  
Chizuka Ide ◽  
Kenichi Kumagai ◽  
Schuichiro Hayashi
Keyword(s):  
Freeze Fracture ◽  
Fracture Study

Mast cell heterogeneity

1984 ◽  
Vol 62 (6) ◽  
pp. 734-737 ◽  
Author(s):  
F. Shanahan ◽  
J. A. Denburg ◽  
J. Bienenstock ◽  
A. D. Befus
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Connective Tissue ◽  
Regulatory Peptides ◽  
Cell Heterogeneity ◽  
Cell Secretion ◽  
Biochemical Basis ◽  
Mucosal Mast Cells ◽  
Mast Cell Heterogeneity

Increasing evidence for the existence of inter- and intra-species mast cell heterogeneity has expanded the potential biological role of this cell. Early studies suggesting that mast cells at mucosal sites differ morphologically and histochemically from connective tissue mast cells have been confirmed using isolated intestinal mucosal mast cells in the rat and more recently in man. These studies also established that mucosal mast cells are functionally distinct from connective tissue mast cells. Thus, mucosal and connective tissue mast cells differ in their responsiveness to a variety of mast cell secretagogues and antiallergic agents. Speculation about the therapeutic use of antiallergic drugs in disorders involving intestinal mast cells cannot, therefore, be based on extrapolation from studies of their effects on mast cells from other sites. Regulatory mechanisms for mast cell secretion may also be heterogeneous since mucosal mast cells differ from connective tissue mast cells in their response to a variety of physiologically occurring regulatory peptides. The development of techniques to purify isolated mast cell sub-populations will facilitate future analysis of the biochemical basis of the functional heterogeneity of mast cells.

Dual effect of spermine on mast cell secretion exhibits different calcium and temperature requirements

1999 ◽  
Vol 21 (9) ◽  
pp. 547-559 ◽  
Author(s):  
Harissis Vliagoftis ◽  
Linda Mak ◽  
William Boucher ◽  
Theoharis C. Theoharides
Keyword(s):  
Mast Cell ◽  
Cell Secretion ◽  
Dual Effect ◽  
Temperature Requirements

Immunoglobulin E Mediates Pulmonary Vascular Remodeling by Upregulating Mast Cell Secretion of VEGFC

2021 ◽  
Author(s):  
Ying Liu ◽  
Ting Shu ◽  
Jinqiu Li ◽  
Jiawei Zhang ◽  
Yitian Zhou ◽  
...  
Keyword(s):  
Mast Cell ◽  
Vascular Remodeling ◽  
Immunoglobulin E ◽  
Cell Secretion ◽  
Pulmonary Vascular Remodeling
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