scholarly journals Membrane particle aggregates in innervated and noninnervated cultures of Xenopus embryonic muscle cells.

1978 ◽  
Vol 75 (1) ◽  
pp. 500-504 ◽  
Author(s):  
H. B. Peng ◽  
Y. Nakajima
1957 ◽  
Vol 3 (2) ◽  
pp. 193-202 ◽  
Author(s):  
Alan R. Muir

Prenatal and postnatal cardiac muscle from rabbits has been studied by electron microscopy, after osmium fixation and methacrylate embedding. The observations showed that 1. Cell membranes divide the muscle into cellular units from the youngest embryo which was studied (9½ days after coitus) until the adult state. 2. The embryonic muscle cells contain only one nucleus, whereas the adult cell may be multinucleated. 3. At all stages of development, wherever a myofibrillar axis crosses a cellular boundary, the myofilaments are interrupted by an intercalated disc. 4. With age, increase in size and complexity of the discs render them recognisable by the light microscope.


1972 ◽  
Vol 52 (3) ◽  
pp. 589-597 ◽  
Author(s):  
Frank E. Stockdale ◽  
Michael C. O'neill

The differentiation of embryonic skeletal muscle cells is closely coupled with the cessation of normal DNA replication. Once these cells begin to differentiate, they normally never undergo semiconservative replication of DNA during the entire life time of the muscle cell. Cessation of DNA synthesis has been shown to be accompanied by a loss of 80–90% of the replicative DNA polymerase activity of these cells. Despite this loss the studies reported here demonstrate that muscle cells retain the ability to synthesize DNA of a repair type after UV irradiation. These results suggest that the control exercised over semiconservative DNA synthesis during differentiation of these cells does not extend to repair synthesis after UV irradiation.


1982 ◽  
Vol 92 (1) ◽  
pp. 231-236 ◽  
Author(s):  
J Prives ◽  
A B Fulton ◽  
S Penman ◽  
M P Daniels ◽  
C N Christian

To monitor the interaction of cell surface acetylcholine (AcCho) receptors with the cytoskeleton, cultured muscle cells were labeled with radioactive or fluorescent alpha-bungarotoxin and extracted with Triton X-100, using conditions that preserve internal structure. A significant population of the AcCho receptors is retained on the skeletal framework remaining after detergent extraction. The skeleton organization responsible for restricting AcCho receptors to a patched region may also result in their retention after detergent extraction.


1978 ◽  
Vol 126 (3) ◽  
pp. 415-431 ◽  
Author(s):  
Robert G. Whalen ◽  
Gillian S. Butler-Browne ◽  
François Gros

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