scholarly journals Membrane lipid heterogeneity associated with acetylcholine receptor particle aggregates in Xenopus embryonic muscle cells.

1981 ◽  
Vol 78 (2) ◽  
pp. 1278-1282 ◽  
Author(s):  
P. C. Bridgman ◽  
Y. Nakajima
Keyword(s):  
Acetylcholine Receptor ◽  
Muscle Cells ◽  
Membrane Lipid ◽  
Particle Aggregates ◽  
Embryonic Muscle

scholarly journals Interaction of the cytoskeletal framework with acetylcholine receptor on th surface of embryonic muscle cells in culture.

1982 ◽  
Vol 92 (1) ◽  
pp. 231-236 ◽  
Author(s):  
J Prives ◽  
A B Fulton ◽  
S Penman ◽  
M P Daniels ◽  
C N Christian
Keyword(s):  
Cell Surface ◽  
Internal Structure ◽  
Acetylcholine Receptor ◽  
Muscle Cells ◽  
Cells In Culture ◽  
Detergent Extraction ◽  
Embryonic Muscle ◽  
Alpha Bungarotoxin ◽  
Triton X

To monitor the interaction of cell surface acetylcholine (AcCho) receptors with the cytoskeleton, cultured muscle cells were labeled with radioactive or fluorescent alpha-bungarotoxin and extracted with Triton X-100, using conditions that preserve internal structure. A significant population of the AcCho receptors is retained on the skeletal framework remaining after detergent extraction. The skeleton organization responsible for restricting AcCho receptors to a patched region may also result in their retention after detergent extraction.

scholarly journals Freeze-fracture and electrophysiological studies of newly developed acetylcholine receptors in Xenopus embryonic muscle cells.

1984 ◽  
Vol 98 (6) ◽  
pp. 2160-2173 ◽  
Author(s):  
P C Bridgman ◽  
S Nakajima ◽  
A S Greenberg ◽  
Y Nakajima
Keyword(s):  
Size Distribution ◽  
Acetylcholine Receptor ◽  
Acetylcholine Receptors ◽  
Freeze Fracture ◽  
Muscle Cells ◽  
Embryonic Muscle ◽  
Freeze Fracture Electron Microscopy ◽  
Receptor Clusters ◽  
Alpha Bungarotoxin ◽  
Solitary Form

The development of acetylcholine receptors on Xenopus embryonic muscle cells both in culture and in situ was studied using electrophysiology and freeze-fracture electron microscopy. Acetylcholine sensitivity first appeared at developmental stage 20 and gradually increased up to about stage 31. Freeze-fracture of muscle cells that were nonsensitive to acetylcholine revealed diffusely distributed small P-face intramembraneous particles. When cells acquired sensitivity to acetylcholine, a different group of diffusely distributed large P-face particles began to appear. This group of particles was analyzed by subtracting the size distribution found on nonsensitive cells from that found on sensitive cells. We call this group of particles difference particles. The sizes of difference particles were large (peak diameter 11 nm). The density of difference particles gradually increased with development. The density of small particles (less than 9 nm) did not change with development. At later stages (32-36) aggregates of large particles appeared, which probably represent acetylcholine receptor clusters. The size distribution of difference particles was close to that of the aggregated particles, suggesting that at least part of difference particles represent diffusely distributed acetylcholine receptors. Difference particles exist mostly in solitary form (occasionally double), indicating that an acetylcholine receptor can be functional in solitary form. This result also shows that diffuse acetylcholine receptors that have previously been observed with 125I-alpha-bungarotoxin autoradiography do indeed exist in solitary forms not as microaggregates.

scholarly journals Synthesis of acetylcholine receptor during differentiation of cultured embryonic muscle cells.

1975 ◽  
Vol 72 (10) ◽  
pp. 4028-4032 ◽  
Author(s):  
J. P. Merlie ◽  
A. Sobel ◽  
J. P. Changeux ◽  
F. Gros
Keyword(s):  
Acetylcholine Receptor ◽  
Muscle Cells ◽  
Embryonic Muscle
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