scholarly journals Identification of a cDNA clone for mouse apoprotein A-1 (apo A-1) and its use in characterization of apo A-1 mRNA expression in liver and small intestine.

1983 ◽  
Vol 80 (6) ◽  
pp. 1511-1515 ◽  
Author(s):  
J. C. Miller ◽  
R. K. Barth ◽  
P. H. Shaw ◽  
R. W. Elliott ◽  
N. D. Hastie
Keyword(s):  
Small Intestine ◽  
Mrna Expression ◽  
Cdna Clone

Galectin-3 is modulated in pancreatic cancer cells under hypoxia and nutrient deprivation

2020 ◽  
Vol 401 (10) ◽  
pp. 1153-1165 ◽  
Author(s):  
Antônio F. da Silva Filho ◽  
Lucas B. Tavares ◽  
Maira G. R. Pitta ◽  
Eduardo I. C. Beltrão ◽  
Moacyr J. B. M. Rêgo
Keyword(s):  
Pancreatic Cancer ◽  
Cell Death ◽  
Mrna Expression ◽  
Cancer Cells ◽  
Ductal Adenocarcinoma ◽  
Reverse Transcription Pcr ◽  
Pancreatic Cancer Cells ◽  
Through Flow ◽  
Galectin 3

AbstractPancreatic ductal adenocarcinoma is one of the most aggressive tumors with a microenvironment marked by hypoxia and starvation. Galectin-3 has been evaluated in solid tumors and seems to present both pro/anti-tumor effects. So, this study aims to characterize the expression of Galectin-3 from pancreatic tumor cells and analyze its influence for cell survive and motility in mimetic microenvironment. For this, cell cycle and cell death were accessed through flow cytometry. Characterization of inside and outside Galectin-3 was performed through Real-Time Quantitative Reverse Transcription PCR (qRT-PCR), immunofluorescence, Western blot, and ELISA. Consequences of Galectin-3 extracellular inhibition were investigated using cell death and scratch assays. PANC-1 showed increased Galectin-3 mRNA expression when cultivated in hypoxia for 24 and 48 h. After 24 h in simultaneously hypoxic/deprived incubation, PANC-1 shows increased Galectin-3 protein and secreted levels. For Mia PaCa-2, cultivation in deprivation was determinant for the increasing in Galectin-3 mRNA expression. When cultivated in simultaneously hypoxic/deprived condition, Mia PaCa-2 also presented increasing for the Galectin-3 secreted levels. Treatment of PANC-1 cells with lactose increased the death rate when cells were incubated simultaneously hypoxic/deprived condition. Therefore, it is possible to conclude that the microenvironmental conditions modulate the Galectin-3 expression on the transcriptional and translational levels for pancreatic cancer cells.

Region-dependent disappearance of vinblastine in rat small intestine and characterization of its P-glycoprotein-mediated efflux system

2000 ◽  
Vol 11 (4) ◽  
pp. 317-324 ◽  
Author(s):  
Akira Nakayama ◽  
Hiroshi Saitoh ◽  
Masako Oda ◽  
Masahiko Takada ◽  
Bruce J Aungst
Keyword(s):  
Small Intestine ◽  
Rat Small Intestine ◽  
Efflux System ◽  
P Glycoprotein

Immunohistochemical characterization of putative primary afferent (sensory) myenteric neurons in human small intestine

2004 ◽  
Vol 112 (1-2) ◽  
pp. 49-59 ◽  
Author(s):  
Axel Brehmer ◽  
Roland Croner ◽  
Arno Dimmler ◽  
Thomas Papadopoulos ◽  
Falk Schrödl ◽  
...  
Keyword(s):  
Small Intestine ◽  
Primary Afferent ◽  
Myenteric Neurons ◽  
Human Small Intestine

mRNA expression profiles of P450 3A enzymes in the liver and small intestine of the domestic pig

2012 ◽  
Vol 93 (1) ◽  
pp. 360-365 ◽  
Author(s):  
Min Yao ◽  
Menghong Dai ◽  
Zhaoying Liu ◽  
Wenlong Cui ◽  
Daoyuan Li ◽  
...  
Keyword(s):  
Small Intestine ◽  
Mrna Expression ◽  
Expression Profiles ◽  
Domestic Pig

Isolation and characterization of a tobacco cDNA clone encoding a putative MAP kinase

1993 ◽  
Vol 23 (3) ◽  
pp. 543-551 ◽  
Author(s):  
Cathal Wilson ◽  
Norbert Eller ◽  
Anton Gartner ◽  
Oscar Vicente ◽  
Erwin Heberle-Bors
Keyword(s):  
Map Kinase ◽  
Cdna Clone ◽  
Isolation And Characterization
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