Asbestos fibers mediate transformation of monkey cells by exogenous plasmid DNA.

The fate of exogenous DNA introduced into Chlamydomonas reinhardtii by electroporation was analyzed. With single and double electrical pulses, plasmids as large as 14 kb were introduced into cells with and without intact cell walls. Within hours after introduction, exogenous plasmid DNA was associated with nuclei isolated from cells; several weeks after introduction, exogenous DNA was stably integrated into the Chlamydomonas genome. These studies establish electroporation as a method for introducing DNA, and potentially other molecules, into C. reinhardtii.

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Chitin Nanowhiskers Mediate Transformation of Escherichia coli by Exogenous Plasmid DNA

Journal of Biotechnology & Biomaterials ◽

10.4172/2155-952x.1000114 ◽

2011 ◽

Vol 01 (06) ◽

Cited By ~ 5

Author(s):

Alisa Mera ◽

Jun Araki ◽

Takashi Ohtsuki

Keyword(s):

Escherichia Coli ◽

Plasmid Dna ◽

Chitin Nanowhiskers ◽

Exogenous Plasmid

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341. Exogenous Plasmid DNA Sequences Differentially Affect Episomal Transgene Silencing in Liver and Muscle

Molecular Therapy ◽

10.1016/s1525-0016(16)44547-8 ◽

2007 ◽

Vol 15 ◽

pp. S129

Keyword(s):

Dna Sequences ◽

Plasmid Dna ◽

Transgene Silencing ◽

Exogenous Plasmid

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Enhanced Agrobacterium ‐mediated transformation revealed attenuation of exogenous plasmid DNA installation in recipient bacteria by exonuclease VII and SbcCD

Genes to Cells ◽

10.1111/gtc.12802 ◽

2020 ◽

Vol 25 (10) ◽

pp. 663-674

Author(s):

Kazuya Kiyokawa ◽

Yuta Ohmine ◽

Kazuya Yunoki ◽

Shinji Yamamoto ◽

Kazuki Moriguchi ◽

...

Keyword(s):

Plasmid Dna ◽

Agrobacterium Mediated Transformation ◽

Exonuclease Vii ◽

Exogenous Plasmid

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Introduction of exogenous DNA into Chlamydomonas reinhardtii by electroporation.

Molecular and Cellular Biology ◽

10.1128/mcb.11.4.2328 ◽

1991 ◽

Vol 11 (4) ◽

pp. 2328-2332 ◽

Cited By ~ 77

Author(s):

L E Brown ◽

S L Sprecher ◽

L R Keller

Keyword(s):

Chlamydomonas Reinhardtii ◽

Plasmid Dna ◽

Cell Walls ◽

Intact Cell ◽

Exogenous Dna ◽

Electrical Pulses ◽

Exogenous Plasmid

The fate of exogenous DNA introduced into Chlamydomonas reinhardtii by electroporation was analyzed. With single and double electrical pulses, plasmids as large as 14 kb were introduced into cells with and without intact cell walls. Within hours after introduction, exogenous plasmid DNA was associated with nuclei isolated from cells; several weeks after introduction, exogenous DNA was stably integrated into the Chlamydomonas genome. These studies establish electroporation as a method for introducing DNA, and potentially other molecules, into C. reinhardtii.

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Electron microscopic visualization of sites of nascent DNA synthesis by streptavidin-gold binding to biotinylated nucleotides incorporated in vivo.

The Journal of Cell Biology ◽

10.1083/jcb.107.1.33 ◽

1988 ◽

Vol 107 (1) ◽

pp. 33-44 ◽

Cited By ~ 20

Author(s):

K T Hiriyanna ◽

J Varkey ◽

M Beer ◽

R M Benbow

Keyword(s):

Xenopus Laevis ◽

Plasmid Dna ◽

Early Embryogenesis ◽

Nucleic Acid Detection ◽

Nucleoside Triphosphate ◽

Chromosomal Dna ◽

Electron Microscopic ◽

Detection Systems ◽

Exogenous Plasmid

Biotinylated nucleotides (bio-11-dCTP, bio-11-dUTP, and bio-7-dATP) were microinjected into unfertilized and fertilized Xenopus laevis eggs. The amounts introduced were comparable to in vivo deoxy-nucleoside triphosphate pools. At various times after microinjection, DNA was extracted from eggs or embryos and subjected to electrophoresis on agarose gels. Newly synthesized biotinylated DNA was analyzed by Southern transfer and visualized using either the BluGENE or Detek-hrp streptavidin-based nucleic acid detection systems. Quantitation of the amount of biotinylated DNA observed at various times showed that the microinjected biotinylated nucleotides were efficiently incorporated in vivo, both into replicating endogenous chromosomal DNA and into replicating microinjected exogenous plasmid DNA. At least one biotinylated nucleotide could be incorporated in vivo for every eight nucleotides of DNA synthesized. Control experiments also showed that heavily biotinylated DNA was not subjected to detectable DNA repair during early embryogenesis (for at least 5 h after activation of the eggs). The incorporated biotinylated nucleotides were visualized by electron microscopy by using streptavidin-colloidal gold or streptavidin-ferritin conjugates to bind specifically to the biotin groups projecting from the newly replicated DNA. The incorporated biotinylated nucleotides were thus made visible as electron-dense spots on the underlying DNA molecules. Biotinylated nucleotides separated by 20-50 bases could be resolved. We conclude that nascent DNA synthesized in vivo in Xenopus laevis eggs can be visualized efficiently and specifically using the techniques described.

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