scholarly journals Chrysotile asbestos fibers mediate transformation ofEscherichia coliby exogenous plasmid DNA

2001 ◽  
Vol 195 (2) ◽  
pp. 133-137 ◽  
Author(s):  
Naoto Yoshida ◽  
Takayuki Ikeda ◽  
Terutoyo Yoshida ◽  
Takahumi Sengoku ◽  
Kihachiro Ogawa
Keyword(s):  
Plasmid Dna ◽  
Chrysotile Asbestos ◽  
Asbestos Fibers ◽  
Exogenous Plasmid

scholarly journals Asbestos fibers mediate transformation of monkey cells by exogenous plasmid DNA.

1988 ◽  
Vol 85 (20) ◽  
pp. 7670-7674 ◽  
Author(s):  
J. D. Appel ◽  
T. M. Fasy ◽  
D. S. Kohtz ◽  
J. D. Kohtz ◽  
E. M. Johnson
Keyword(s):  
Plasmid Dna ◽  
Asbestos Fibers ◽  
Exogenous Plasmid

Introduction of exogenous DNA into Chlamydomonas reinhardtii by electroporation

1991 ◽  
Vol 11 (4) ◽  
pp. 2328-2332
Author(s):  
L E Brown ◽  
S L Sprecher ◽  
L R Keller
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Plasmid Dna ◽  
Cell Walls ◽  
Intact Cell ◽  
Exogenous Dna ◽  
Electrical Pulses ◽  
Exogenous Plasmid

The fate of exogenous DNA introduced into Chlamydomonas reinhardtii by electroporation was analyzed. With single and double electrical pulses, plasmids as large as 14 kb were introduced into cells with and without intact cell walls. Within hours after introduction, exogenous plasmid DNA was associated with nuclei isolated from cells; several weeks after introduction, exogenous DNA was stably integrated into the Chlamydomonas genome. These studies establish electroporation as a method for introducing DNA, and potentially other molecules, into C. reinhardtii.

Asbestos fiber identification in liver from cholangiocarcinoma patients living in an asbestos polluted area: a preliminary study

2019 ◽  
Vol 105 (5) ◽  
pp. 404-410 ◽  
Author(s):  
Federica Grosso ◽  
Alessandro Croce ◽  
Roberta Libener ◽  
Narciso Mariani ◽  
Massimo Pastormerlo ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Asbestos Exposure ◽  
Optical Microscope ◽  
Variable Pressure ◽  
Chrysotile Asbestos ◽  
Neoplastic Tissue ◽  
Asbestos Fibers ◽  
Preliminary Study ◽  
Scanning Electron

Purpose: To assess whether asbestos fibers may be observed in liver tissue of patients with cholangiocarcinoma (CC) with environmental or working asbestos exposure. Methods: Detection of fibers was performed directly on histologic sections of liver from 7 patients with CC using optical microscope and variable pressure scanning electron microscopy equipped with energy-dispersive spectroscopy (VP-SEM/EDS). All patients were from Casale Monferrato, Italy, a highly asbestos-polluted town. Due to ethical constraints, observers were blinded to patients’ clinical features. Results: Fibers/bundles of fibers of chrysotile were detected in 5 out of 7 patients (71%). The boundary between healthy and neoplastic tissue or the fibrocollagen tissue produced by the neoplasia were identified as areas of fiber incorporation. Conclusions: This study is the first report about the detection of chrysotile asbestos fibers in the liver of patients with CC. Further studies on larger cohorts are needed to corroborate our preliminary findings.

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