scholarly journals Molecular epidemiology of human hepatitis A virus defined by an antigen-capture polymerase chain reaction method.

1990 ◽  
Vol 87 (8) ◽  
pp. 2867-2871 ◽  
Author(s):  
R. W. Jansen ◽  
G. Siegl ◽  
S. M. Lemon
Keyword(s):  
Polymerase Chain Reaction ◽  
Molecular Epidemiology ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Polymerase Chain Reaction Method ◽  
Chain Reaction ◽  
Reaction Method ◽  
Antigen Capture ◽  
Human Hepatitis ◽  
Polymerase Chain

scholarly journals Prevalence of hepatitis A virus in sea food in Iran

2012 ◽  
Vol 64 (4) ◽  
pp. 887-890
Author(s):  
H. Momtaz ◽  
E. Rahimi ◽  
S. Hajian ◽  
M. Momeni
Keyword(s):  
Polymerase Chain Reaction ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Polymerase Chain Reaction Method ◽  
Food Samples ◽  
Chain Reaction ◽  
Reaction Method ◽  
Fresh Fish ◽  
Potential Sources ◽  
Polymerase Chain

The objective of this study was to determine the prevalence of Hepatitis A Virus (HAV) in sea food samples in the Isfahan and Shahrekord townships in Iran. From September 2010 to April 2011, a total of 300 samples of fresh fish, shrimp, crab and lobster were obtained from randomly selected retail stores in the Isfahan and Shahrekord townships in Iran. The samples were tested for the presence of HAV using a reverse transcriptase- polymerase chain reaction method. Out of the total number of samples examined, 8 (2.7%) were found to be positive for HAV. This virus was detected in 5% and 1.7% of fresh fish and shrimp, respectively. This study shows the importance of sea food as potential sources of HAV infection in people in Iran.

scholarly journals Prolonged fecal excretion of hepatitis A virus in adult patients with hepatitis A as determined by polymerase chain reaction

Hepatology ◽  
1996 ◽  
Vol 24 (1) ◽  
pp. 10-13 ◽  
Author(s):  
H Yotsuyanagi ◽  
K Koike ◽  
K Yasuda ◽  
K Moriya ◽  
Y Shintani ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Adult Patients ◽  
Fecal Excretion ◽  
Chain Reaction ◽  
Polymerase Chain

Persistence of Hepatitis A Virus in Oysters†

2003 ◽  
Vol 66 (2) ◽  
pp. 331-334 ◽  
Author(s):  
DAVID H. KINGSLEY ◽  
GARY P. RICHARDS
Keyword(s):  
Polymerase Chain Reaction ◽  
Crassostrea Virginica ◽  
Reverse Transcription ◽  
Hepatitis A ◽  
Infectious Virus ◽  
Hepatitis A Virus ◽  
Complete Removal ◽  
Chain Reaction ◽  
Daily Feeding ◽  
Polymerase Chain

We investigated the ability of hepatitis A virus (HAV) to persist for up to 6 weeks in Eastern oysters (Crassostrea virginica). Viral RNA was detected by reverse transcription–polymerase chain reaction 6 weeks after 16 h of exposure to 90,000 PFU (180 PFU/ml of seawater) of HAV. Assaying for infectious virus in oysters that received a daily feeding of phytoplankton recovered 3,800, 650, and 500 PFU of HAV 1, 2, and 3 weeks after contamination with 90,000 PFU of HAV, respectively. However, no infectious HAV was isolated from oysters 4, 5, or 6 weeks after contamination. These results support the position that shellfish depuration is insufficient for the complete removal of infectious viruses. Extended relay times (in excess of 4 weeks) may be required to produce virologically safe shellfish.

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