scholarly journals Reduced RNA polymerase II transcription in intact and permeabilized Cockayne syndrome group B cells

1997 ◽  
Vol 94 (9) ◽  
pp. 4306-4311 ◽  
Author(s):  
A. S. Balajee ◽  
A. May ◽  
G. L. Dianov ◽  
E. C. Friedberg ◽  
V. A. Bohr
Keyword(s):  
B Cells ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Cockayne Syndrome ◽  
Group B ◽  
Rna Polymerase Ii Transcription

scholarly journals The Stalling of Transcription at Abasic Sites Is Highly Mutagenic

2003 ◽  
Vol 23 (1) ◽  
pp. 382-388 ◽  
Author(s):  
Sung-Lim Yu ◽  
Sung-Keun Lee ◽  
Robert E. Johnson ◽  
Louise Prakash ◽  
Satya Prakash
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Cockayne Syndrome ◽  
Abasic Sites ◽  
Dramatic Rise ◽  
Ap Sites ◽  
Group B

ABSTRACT Abasic (AP) sites represent one of the most frequently formed lesions in DNA. Here, we examine the consequences of the stalling of RNA polymerase II at AP sites in DNA in Saccharomyces cerevisiae. A severe inhibition of transcription occurs in strains that are defective in the removal of AP sites and that also lack the RAD26 gene, a homolog of the human Cockayne syndrome group B (CSB) gene, and, importantly, a dramatic rise in mutagenesis is incurred in such strains. From the various observations presented here, we infer that the stalling of transcription at AP sites is highly mutagenic.

scholarly journals Reduced RNA polymerase II transcription in extracts of Cockayne syndrome and xeroderma pigmentosum/Cockayne syndrome cells

1997 ◽  
Vol 25 (18) ◽  
pp. 3636-3642 ◽  
Author(s):  
G. L. Dianov ◽  
J.-F. Houle ◽  
N. Iyer ◽  
V. A. Bohr ◽  
E. C. Friedberg
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Xeroderma Pigmentosum ◽  
Cockayne Syndrome ◽  
Rna Polymerase Ii Transcription

scholarly journals Evidence that the Transcription Elongation Function of Rpb9 Is Involved in Transcription-Coupled DNA Repair in Saccharomyces cerevisiae

2006 ◽  
Vol 26 (24) ◽  
pp. 9430-9441 ◽  
Author(s):  
Shisheng Li ◽  
Baojin Ding ◽  
Runqiang Chen ◽  
Christine Ruggiero ◽  
Xuefeng Chen
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Repair ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Transcription Elongation ◽  
Elongation Factor ◽  
Complementation Group ◽  
Cockayne Syndrome ◽  
Transcription Repression ◽  
Group B

ABSTRACT Rpb9, a small nonessential subunit of RNA polymerase II, has been shown to have multiple transcription-related functions in Saccharomyces cerevisiae. These functions include promoting transcription elongation and mediating a subpathway of transcription-coupled repair (TCR) that is independent of Rad26, the homologue of human Cockayne syndrome complementation group B protein. Rpb9 is composed of three distinct domains: the N-terminal Zn1, the C-terminal Zn2, and the central linker. Here we show that the Zn1 and linker domains are essential, whereas the Zn2 domain is almost dispensable, for both transcription elongation and TCR functions. Impairment of transcription elongation, which does not dramatically compromise Rad26-mediated TCR, completely abolishes Rpb9-mediated TCR. Furthermore, Rpb9 appears to be dispensable for TCR if its transcription elongation function is compensated for by removing a transcription repression/elongation factor. Our data suggest that the transcription elongation function of Rpb9 is involved in TCR.

scholarly journals A role for the Cockayne Syndrome B (CSB)-Elongin ubiquitin ligase complex in signal-dependent RNA polymerase II transcription

2021 ◽  
pp. 100862
Author(s):  
Juston C. Weems ◽  
Brian D. Slaughter ◽  
Jay R. Unruh ◽  
Kyle J. Weaver ◽  
Brandon D. Miller ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Ubiquitin Ligase ◽  
Cockayne Syndrome ◽  
Ubiquitin Ligase Complex ◽  
Rna Polymerase Ii Transcription
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