scholarly journals Characterization of lipid bilayer phases by confocal microscopy and fluorescence correlation spectroscopy

1999 ◽  
Vol 96 (15) ◽  
pp. 8461-8466 ◽  
Author(s):  
J. Korlach ◽  
P. Schwille ◽  
W. W. Webb ◽  
G. W. Feigenson
Keyword(s):  
Confocal Microscopy ◽  
Lipid Bilayer ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation

scholarly journals Advanced processing and analysis of conventional confocal microscopy generated scanning FCS data

2017 ◽  
Author(s):  
Dominic Waithe ◽  
Falk Schneider ◽  
Jakub Chojnacki ◽  
Mathias Clausen ◽  
Dilip Shrestha ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Spatial Heterogeneity ◽  
Fluorescence Correlation Spectroscopy ◽  
Large Scale ◽  
Molecular Diffusion ◽  
Correlation Spectroscopy ◽  
The Other ◽  
Statistical Accuracy ◽  
Fluorescence Correlation ◽  
Diffusion Dynamics

AbstractScanning Fluorescence Correlation Spectroscopy (scanning FCS) is a variant of conventional point FCS that allows molecular diffusion at multiple locations to be measured simultaneously. It enables disclosure of potential spatial heterogeneity in molecular diffusion dynamics and also the acquisition of a large amount of FCS data at the same time, providing large statistical accuracy. Here, we optimize the processing and analysis of these large-scale acquired sets of FCS data. On one hand we present FoCuS-scan, scanning FCS software that provides an end-to-end solution for processing and analysing scanning data acquired on commercial turnkey confocal systems. On the other hand, we provide a thorough characterisation of large-scale scanning FCS data over its intended time-scales and applications and propose a unique solution for the bias and variance observed when studying slowly diffusing species. Our manuscript enables researchers to straightforwardly utilise scanning FCS as a powerful technique for measuring diffusion across a broad range of physiologically relevant length scales without specialised hardware or expensive software.

Characterization of Pteridines: a New Approach by Fluorescence Correlation Spectroscopy and Analysis of Assay Sensitivity

Pteridines ◽  
2001 ◽  
Vol 12 (4) ◽  
pp. 147-153 ◽  
Author(s):  
U. Demel ◽  
Z. Foldes-Papp ◽  
D. Fuchs ◽  
G. P. Tilz
Keyword(s):  
Single Molecule ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Cell Mediated Immunity ◽  
Distribution Analysis ◽  
Assay Sensitivity ◽  
New Approach ◽  
Single Molecule Level ◽  
Fluorescence Correlation

Abstract In the present investigation, fluorescence con-elation spectroscopy (FCS) was used to measure the molecular motion of the pteridine derivative neopterin. However, technical limitations in the present optical setup precluded the identification of ,single neopterin molecules. FCS measurements with a fluorophore were also can-ied out for comparison. Exemplified by rhodamine green, we have introduced a concept that allows the detection, identification and analysis of assays in solution at the single-molecule level in tenns of bulk concentration. This concept is based on FCS and Poisson distribution analysis of assay sensitivity. The molecules had not to be quantified in a more concentrated fonn, or in flow and trapping experiments. The study demonstrated an ultrasensitive, reliable, rapid and direct tool for analytics and diagnostics in solution. We discuss a possible application of our new concept in activation control of cell-mediated immunity via neopterin determination.

scholarly journals Characterization of Porous Materials by Fluorescence Correlation Spectroscopy Super-resolution Optical Fluctuation Imaging

ACS Nano ◽  
2015 ◽  
Vol 9 (9) ◽  
pp. 9158-9166 ◽  
Author(s):  
Lydia Kisley ◽  
Rachel Brunetti ◽  
Lawrence J. Tauzin ◽  
Bo Shuang ◽  
Xiyu Yi ◽  
...  
Keyword(s):  
Porous Materials ◽  
Fluorescence Correlation Spectroscopy ◽  
Super Resolution ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation

Calibration and Limits of Camera-Based Fluorescence Correlation Spectroscopy: A Supported Lipid Bilayer Study

ChemPhysChem ◽  
2012 ◽  
Vol 13 (11) ◽  
pp. 2784-2794 ◽  
Author(s):  
Nirmalya Bag ◽  
Jagadish Sankaran ◽  
Alexandra Paul ◽  
Rachel S. Kraut ◽  
Thorsten Wohland
Keyword(s):  
Lipid Bilayer ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Supported Lipid Bilayer ◽  
Fluorescence Correlation

Fluorescence Correlation Spectroscopy for Detection of Trace Amount of Biological Agents

2002 ◽  
Author(s):  
De-Kui Qing ◽  
M. Pinar Mengu¨c¸ ◽  
Fred A. Payne ◽  
Mary-Grace C. Danao
Keyword(s):  
Confocal Microscopy ◽  
Fluorescence Correlation Spectroscopy ◽  
Trace Amount ◽  
Flow System ◽  
Detection System ◽  
Biological Agents ◽  
Correlation Spectroscopy ◽  
Special Flow ◽  
E Coli ◽  
Fluorescence Correlation

A procedure to the trace amount of biological agents in water, based on fluorescence correlation spectroscopy and confocal microscopy, is explored. A special flow system is developed to increase the probability of detecting Escherichia coli (E. coli), and confocal microscopy is used to increase the sensitivity of the detection system. It is observed that concentrations higher than 1.5×105 E. coli per milliliter (2.5×10−16 M) are detectable with the present setup. This concentration corresponds to about 1.0 nM level of Rhodamine 6-G dyes. For detection of lower concentrations, further improvements need to be implemented to increase signal/background ratio. A detailed analysis of the optical system is presented and further improvements for the procedure are discussed.

Characterization of the Triplet State of Hybridization-Sensitive DNA Probe by Using Fluorescence Correlation Spectroscopy

2012 ◽  
Vol 117 (1) ◽  
pp. 27-33 ◽  
Author(s):  
Hyo-Sup Shin ◽  
Akimitsu Okamoto ◽  
Yasushi Sako ◽  
Sok Won Kim ◽  
Soo Yong Kim ◽  
...  
Keyword(s):  
Triplet State ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Dna Probe ◽  
Fluorescence Correlation
Sign in / Sign up

Export Citation Format

Share Document