scholarly journals Characterization of Porous Materials by Fluorescence Correlation Spectroscopy Super-resolution Optical Fluctuation Imaging

ACS Nano ◽  
2015 ◽  
Vol 9 (9) ◽  
pp. 9158-9166 ◽  
Author(s):  
Lydia Kisley ◽  
Rachel Brunetti ◽  
Lawrence J. Tauzin ◽  
Bo Shuang ◽  
Xiyu Yi ◽  
...  
Keyword(s):  
Porous Materials ◽  
Fluorescence Correlation Spectroscopy ◽  
Super Resolution ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation

Characterization of Pteridines: a New Approach by Fluorescence Correlation Spectroscopy and Analysis of Assay Sensitivity

Pteridines ◽  
2001 ◽  
Vol 12 (4) ◽  
pp. 147-153 ◽  
Author(s):  
U. Demel ◽  
Z. Foldes-Papp ◽  
D. Fuchs ◽  
G. P. Tilz
Keyword(s):  
Single Molecule ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Cell Mediated Immunity ◽  
Distribution Analysis ◽  
Assay Sensitivity ◽  
New Approach ◽  
Single Molecule Level ◽  
Fluorescence Correlation

Abstract In the present investigation, fluorescence con-elation spectroscopy (FCS) was used to measure the molecular motion of the pteridine derivative neopterin. However, technical limitations in the present optical setup precluded the identification of ,single neopterin molecules. FCS measurements with a fluorophore were also can-ied out for comparison. Exemplified by rhodamine green, we have introduced a concept that allows the detection, identification and analysis of assays in solution at the single-molecule level in tenns of bulk concentration. This concept is based on FCS and Poisson distribution analysis of assay sensitivity. The molecules had not to be quantified in a more concentrated fonn, or in flow and trapping experiments. The study demonstrated an ultrasensitive, reliable, rapid and direct tool for analytics and diagnostics in solution. We discuss a possible application of our new concept in activation control of cell-mediated immunity via neopterin determination.

scholarly journals Diffusion and interaction dynamics of the cytosolic peroxisomal import receptor PEX5

2021 ◽  
Author(s):  
Silvia Galiani ◽  
Katharina Reglinski ◽  
Pablo Carravilla ◽  
Aurelien Barbotin ◽  
Iztok Urbančič ◽  
...  
Keyword(s):  
Molecular Interactions ◽  
Super Resolution ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation ◽  
Interaction Dynamics ◽  
Diffusion Dynamics ◽  
Interaction Partners ◽  
Target Binding ◽  
Import Receptor

Measuring diffusion dynamics in living cells is essential for the understanding of molecular interactions. While various techniques have been used to explore such characteristics in the plasma membrane, this is less developed for measurements inside the cytosol. An example of cytosolic action is the import of proteins into peroxisomes, via the peroxisomal import receptor PEX5. Here, we combined advanced microscopy and spectroscopy techniques such as fluorescence correlation spectroscopy (FCS) and super-resolution STED microscopy to present a detailed characterization of the diffusion and interaction dynamics of PEX5. Among other features, we disclose a slow diffusion of PEX5, independent of aggregation or target binding, but associated with cytosolic interaction partners via its N-terminal domain. This sheds new light on the functionality of the receptor in the cytosol. Besides specific insights, our study highlights the potential of using complementary microscopy tools to decipher molecular interactions in the cytosol via studying their diffusion dynamics.

Characterization of the Triplet State of Hybridization-Sensitive DNA Probe by Using Fluorescence Correlation Spectroscopy

2012 ◽  
Vol 117 (1) ◽  
pp. 27-33 ◽  
Author(s):  
Hyo-Sup Shin ◽  
Akimitsu Okamoto ◽  
Yasushi Sako ◽  
Sok Won Kim ◽  
Soo Yong Kim ◽  
...  
Keyword(s):  
Triplet State ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Dna Probe ◽  
Fluorescence Correlation
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