scholarly journals Padlock oligonucleotides for duplex DNA based on sequence-specific triple helix formation

1999 ◽  
Vol 96 (19) ◽  
pp. 10603-10607 ◽  
Author(s):  
C. Escude ◽  
T. Garestier ◽  
C. Helene
Keyword(s):  
Triple Helix ◽  
Duplex Dna ◽  
Helix Formation ◽  
Triple Helix Formation

Deoxyoligonucleotides bearing neutral analogs of phosphodiester linkages recognize duplex DNA via triple-helix formation

1991 ◽  
Vol 113 (20) ◽  
pp. 7767-7768 ◽  
Author(s):  
Mark Matteucci ◽  
Kuei Ying Lin ◽  
Samuel Butcher ◽  
Courtney Moulds
Keyword(s):  
Triple Helix ◽  
Duplex Dna ◽  
Helix Formation ◽  
Triple Helix Formation

Selective reaction to a flipping cytidine of the duplex DNA mediated by triple helix formation

2001 ◽  
Vol 11 (3) ◽  
pp. 343-345 ◽  
Author(s):  
Fumi Nagatsugi ◽  
Daisaku Usui ◽  
Takeshi Kawasaki ◽  
Minoru Maeda ◽  
Shigeki Sasaki
Keyword(s):  
Triple Helix ◽  
Duplex Dna ◽  
Helix Formation ◽  
Selective Reaction ◽  
Triple Helix Formation

Formation of stable DNA triplexes

2011 ◽  
Vol 39 (2) ◽  
pp. 629-634 ◽  
Author(s):  
Keith R. Fox ◽  
Tom Brown
Keyword(s):  
Triple Helix ◽  
Low Ph ◽  
Cross Linking ◽  
Duplex Dna ◽  
Sequence Specificity ◽  
Modified Oligonucleotides ◽  
Major Groove ◽  
Dna Triplexes ◽  
Helix Formation ◽  
Triple Helix Formation

Triple-helical nucleic acids are formed by binding an oligonucleotide within the major groove of duplex DNA. These complexes offer the possibility of designing oligonucleotides which bind to duplex DNA with considerable sequence specificity. However, triple-helix formation with natural nucleotides is limited by (i) the requirement for low pH, (ii) the requirement for homopurine target sequences, and (iii) their relatively low affinity. We have prepared modified oligonucleotides to overcome these limitations, including the addition of positive charges to the sugar and/or base, the inclusion of cytosine analogues, the development of nucleosides for recognition of pyrimidine interruptions and the attachment of one or more cross-linking groups. By these means we are able to generate triplexes which have high affinities at physiological pH at sequences that contain pyrimidine interruptions.

Nonenzymatic sequence-specific cleavage of duplex DNA via triple-helix formation by homopyrimidine phosphorothioate oligonucleotides

1996 ◽  
Vol 4 (12) ◽  
pp. 2219-2224 ◽  
Author(s):  
Satoru Tsukahara ◽  
Junji Suzuki ◽  
Kaoru Ushijima ◽  
Kazuyuki Takai ◽  
Hiroshi Takaku
Keyword(s):  
Triple Helix ◽  
Duplex Dna ◽  
Helix Formation ◽  
Triple Helix Formation
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