scholarly journals Reverse genetic analysis of Caenorhabditis elegans presenilins reveals redundant but unequal roles for sel-12 and hop-1 in Notch-pathway signaling

1999 ◽  
Vol 96 (5) ◽  
pp. 2497-2502 ◽  
Author(s):  
B. Westlund ◽  
D. Parry ◽  
R. Clover ◽  
M. Basson ◽  
C. D. Johnson
Keyword(s):  
Caenorhabditis Elegans ◽  
Genetic Analysis ◽  
Notch Pathway ◽  
Reverse Genetic

Reverse Genetic Analysis of the Caenorhabditis elegans 26S Proteasome Subunits by RNA Interference

2002 ◽  
Vol 383 (7-8) ◽  
pp. 1263-1266 ◽  
Author(s):  
M. Takahashi ◽  
H. Iwasaki ◽  
H. Inoue ◽  
K. Takahashi
Keyword(s):  
Rna Interference ◽  
Caenorhabditis Elegans ◽  
Genetic Analysis ◽  
Postembryonic Development ◽  
26S Proteasome ◽  
Reverse Genetic ◽  
Proteasome Subunit ◽  
Proteasome Subunits ◽  
Regulatory Particle

Abstract Reverse genetic analysis was performed on the Caenorhabditis elegans 26S proteasome subunit genes by doublestranded RNAmediated interference (RNAi). Embryonic and postembryonic lethality was caused by interference of all of the eight tested 20S core subunits and all of the 19S regulatory particle subunits except for CeRpn9, CeRpn10, and Ce Rpn12, where RNAi caused no abnormality. However, synthetic suppression of CeRpn10 and CeRpn12 was lethal, whereas neither the combination of Ce Rpn9 with CeRpn10 nor with CeRpn12 resulted in abnormalities in RNAi. These results indicate that the 26S proteasome is indispensable for embryogenesis and postembryonic development, although Ce Rpn9, CeRpn10, and CeRpn12 are not essential, at least under the conditions used. CeRpn10 and Ce Rpn12 are considered to compensate for the suppression of each other.

scholarly journals A Reverse Genetic Analysis of Components of the Toll Signaling Pathway in Caenorhabditis elegans

2006 ◽  
Vol 16 (14) ◽  
pp. 1477 ◽  
Author(s):  
Nathalie Pujol ◽  
Elizabeth M. Link ◽  
Leo X. Liu ◽  
C. Léopold Kurz ◽  
Geneviève Alloing ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Genetic Analysis ◽  
Signaling Pathway ◽  
Reverse Genetic ◽  
Toll Signaling

scholarly journals A reverse genetic analysis of components of the Toll signaling pathway in Caenorhabditis elegans

2001 ◽  
Vol 11 (11) ◽  
pp. 809-821 ◽  
Author(s):  
Nathalie Pujol ◽  
Elizabeth M. Link ◽  
Leo X. Liu ◽  
C.Léopold Kurz ◽  
Geneviève Alloing ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Genetic Analysis ◽  
Signaling Pathway ◽  
Reverse Genetic ◽  
Toll Signaling

scholarly journals Microinjection of mRNA or morpholinos for reverse genetic analysis in the starlet sea anemone, Nematostella vectensis

2013 ◽  
Vol 8 (5) ◽  
pp. 924-934 ◽  
Author(s):  
Michael J Layden ◽  
Eric Röttinger ◽  
Francis S Wolenski ◽  
Thomas D Gilmore ◽  
Mark Q Martindale
Keyword(s):  
Genetic Analysis ◽  
Sea Anemone ◽  
Nematostella Vectensis ◽  
Reverse Genetic

scholarly journals Reverse Genetic Analysis of the Transcription Regulatory Sequence of the Coronavirus Transmissible Gastroenteritis Virus

2004 ◽  
Vol 78 (11) ◽  
pp. 6061-6066 ◽  
Author(s):  
Kristopher M. Curtis ◽  
Boyd Yount ◽  
Amy C. Sims ◽  
Ralph S. Baric
Keyword(s):  
Genetic Analysis ◽  
Full Length ◽  
Transmissible Gastroenteritis Virus ◽  
Regulatory Sequence ◽  
Reverse Genetic ◽  
Conserved Sequence ◽  
Gastroenteritis Virus ◽  
Flanking Sequences ◽  
Transmissible Gastroenteritis ◽  
Discontinuous Transcription

ABSTRACT Coronavirus discontinuous transcription uses a highly conserved sequence (CS) in the joining of leader and body RNAs. Using a full-length infectious construct of transmissable gastroenteritis virus, the present study demonstrates that subgenomic transcription is heavily influenced by upstream flanking sequences and supports a mechanism of transcription attenuation that is regulated in part by a larger domain composed of primarily upstream flanking sequences which select appropriately positioned CS elements for synthesis of subgenomic RNAs.

Sign in / Sign up

Export Citation Format

Share Document