scholarly journals A mutant herpes simplex virus type 1 thymidine kinase reporter gene shows improved sensitivity for imaging reporter gene expression with positron emission tomography

2000 ◽  
Vol 97 (6) ◽  
pp. 2785-2790 ◽  
Author(s):  
S. S. Gambhir ◽  
E. Bauer ◽  
M. E. Black ◽  
Q. Liang ◽  
M. S. Kokoris ◽  
...  
Keyword(s):  
Gene Expression ◽  
Positron Emission Tomography ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Reporter Gene ◽  
Herpes Simplex Virus Type ◽  
Emission Tomography ◽  
Positron Emission ◽  
Simplex Virus

Improved Herpes Simplex Virus Type 1 Amplicon Vectors for Proportional Coexpression of Positron Emission Tomography Marker and Therapeutic Genes

2003 ◽  
Vol 14 (3) ◽  
pp. 277-297 ◽  
Author(s):  
Andreas H. Jacobs ◽  
Alexandra Winkeler ◽  
Markus Hartung ◽  
Mark Slack ◽  
Claus Dittmar ◽  
...  
Keyword(s):  
Positron Emission Tomography ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Emission Tomography ◽  
Positron Emission ◽  
Simplex Virus ◽  
Therapeutic Genes

scholarly journals Imaging progress of herpes simplex virus type 1 thymidine kinase suicide gene therapy in living subjects with positron emission tomography

2004 ◽  
Vol 12 (3) ◽  
pp. 329-339 ◽  
Author(s):  
Shahriar S Yaghoubi ◽  
Jorge R Barrio ◽  
Mohammad Namavari ◽  
Nagichettiar Satyamurthy ◽  
Michael E Phelps ◽  
...  
Keyword(s):  
Positron Emission Tomography ◽  
Gene Therapy ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Herpes Simplex Virus Type ◽  
Suicide Gene ◽  
Emission Tomography ◽  
Positron Emission ◽  
Simplex Virus

scholarly journals Replication of ICP0-Null Mutant Herpes Simplex Virus Type 1 Is Restricted by both PML and Sp100

2007 ◽  
Vol 82 (6) ◽  
pp. 2661-2672 ◽  
Author(s):  
Roger D. Everett ◽  
Carlos Parada ◽  
Philippe Gripon ◽  
Hüseyin Sirma ◽  
Anne Orr
Keyword(s):  
Gene Expression ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Human Fibroblasts ◽  
Null Mutant ◽  
Simplex Virus ◽  
Hsv 1

ABSTRACT Herpes simplex virus type 1 (HSV-1) mutants that fail to express the viral immediate-early protein ICP0 have a pronounced defect in viral gene expression and plaque formation in limited-passage human fibroblasts. ICP0 is a RING finger E3 ubiquitin ligase that induces the degradation of several cellular proteins. PML, the organizer of cellular nuclear substructures known as PML nuclear bodies or ND10, is one of the most notable proteins that is targeted by ICP0. Depletion of PML from human fibroblasts increases ICP0-null mutant HSV-1 gene expression, but not to wild-type levels. In this study, we report that depletion of Sp100, another major ND10 protein, results in a similar increase in ICP0-null mutant gene expression and that simultaneous depletion of both proteins complements the mutant virus to a greater degree. Although chromatin assembly and modification undoubtedly play major roles in the regulation of HSV-1 infection, we found that inhibition of histone deacetylase activity with trichostatin A was unable to complement the defect of ICP0-null mutant HSV-1 in either normal or PML-depleted human fibroblasts. These data lend further weight to the hypothesis that ND10 play an important role in the regulation of HSV-1 gene expression.

Real time PCR for monitoring regulation of host gene expression in herpes simplex virus type 1-infected human diploid cells

2004 ◽  
Vol 118 (2) ◽  
pp. 83-94 ◽  
Author(s):  
Kristina Nyström ◽  
Marlene Biller ◽  
Ammi Grahn ◽  
Magnus Lindh ◽  
Göran Larson ◽  
...  
Keyword(s):  
Gene Expression ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Herpes Simplex Virus Type ◽  
Real Time Pcr ◽  
Host Gene Expression ◽  
Simplex Virus ◽  
Human Diploid Cells ◽  
Diploid Cells
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