scholarly journals Crystal Structure of the Chromodomain Helicase DNA-binding Protein 1 (Chd1) DNA-binding Domain in Complex with DNA

2011 ◽  
Vol 286 (49) ◽  
pp. 42099-42104 ◽  
Author(s):  
Amit Sharma ◽  
Katherine R. Jenkins ◽  
Annie Héroux ◽  
Gregory D. Bowman
Keyword(s):  
Crystal Structure ◽  
Dna Binding ◽  
Binding Protein ◽  
Dna Binding Protein ◽  
Binding Domain ◽  
Dna Binding Domain

scholarly journals Crystal Structure of the DNA-Binding Domain of the Epstein–Barr Virus Origin-Binding Protein, EBNA1, Bound to DNA

Cell ◽  
1996 ◽  
Vol 84 (5) ◽  
pp. 791-800 ◽  
Author(s):  
Alexey Bochkarev ◽  
Jean A Barwell ◽  
Richard A Pfuetzner ◽  
Elena Bochkareva ◽  
Lori Frappier ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Dna Binding ◽  
Epstein Barr Virus ◽  
Binding Protein ◽  
Binding Domain ◽  
Dna Binding Domain ◽  
Barr Virus ◽  
Virus Origin ◽  
Origin Binding Protein ◽  
Epstein Barr

scholarly journals Crystal structure of the DNA-binding domain of the Epstein-Barr virus origin-binding protein EBNA1

Cell ◽  
1995 ◽  
Vol 83 (1) ◽  
pp. 39-46 ◽  
Author(s):  
Alexey Bochkarev ◽  
Jean A. Barwell ◽  
Richard A. Pfuetzner ◽  
William Furey ◽  
Aled M. Edwards ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Dna Binding ◽  
Epstein Barr Virus ◽  
Binding Protein ◽  
Binding Domain ◽  
Dna Binding Domain ◽  
Barr Virus ◽  
Virus Origin ◽  
Origin Binding Protein ◽  
Epstein Barr

scholarly journals The essential DNA-binding protein sap1 of Schizosaccharomyces pombe contains two independent oligomerization interfaces that dictate the relative orientation of the DNA-binding domain.

1995 ◽  
Vol 15 (9) ◽  
pp. 4939-4946 ◽  
Author(s):  
M Ghazvini ◽  
V Ribes ◽  
B Arcangioli
Keyword(s):  
Dna Binding ◽  
Schizosaccharomyces Pombe ◽  
Binding Protein ◽  
Selection Procedure ◽  
Direct Repeat ◽  
Dna Binding Protein ◽  
Binding Domain ◽  
Cooperative Interaction ◽  
Dna Binding Domain ◽  
Dimerization Domain

The sap1 gene from Schizosaccharomyces pombe, which is essential for mating-type switching and for growth, encodes a sequence-specific DNA-binding protein with no homology to other known proteins. We have used a reiterative selection procedure to isolate binding sites for sap1, using a bacterially expressed protein and randomized double-strand oligonucleotides. The sap1 homodimer preferentially selects a pentameric motif, TA(A/G)CG, organized as a direct repeat and spaced by 5 nucleotides. Removal of a C-terminal dimerization domain abolishes recognition of the direct repeat and creates a new specificity for a DNA sequence containing the same pentameric motif but organized as an inverted repeat. We present evidence that the orientation of the DNA-binding domain is controlled by two independent oligomerization interfaces. The C-terminal dimerization domain allows a head-to-tail organization of the DNA-binding domains in solution, while an N-terminal domain is involved in a cooperative interaction on the DNA target between pairs of dimers.

Expression and Purification of the DNA-Binding Domain of SRF: SRF-DB, a Part of a DNA-Binding Protein Which Can Act as a Dominant Negative Mutant in Vivo

1993 ◽  
Vol 209 (2) ◽  
pp. 208-215 ◽  
Author(s):  
Cécile Gauthier-Rouvière ◽  
Caı̈ Qiu-Qiong ◽  
Nicole Lautredou ◽  
Anne Fernandez ◽  
Jean-Marie Blanchard ◽  
...  
Keyword(s):  
Dna Binding ◽  
Binding Protein ◽  
Dna Binding Protein ◽  
Binding Domain ◽  
Dominant Negative ◽  
Dominant Negative Mutant ◽  
Dna Binding Domain ◽  
Expression And Purification ◽  
Negative Mutant

scholarly journals Functional analyses of the Dof domain, a zinc finger DNA-binding domain, in a pumpkin DNA-binding protein AOBP

FEBS Letters ◽  
1998 ◽  
Vol 430 (3) ◽  
pp. 251-256 ◽  
Author(s):  
Naoko Shimofurutani ◽  
Yasutomo Kisu ◽  
Masashi Suzuki ◽  
Muneharu Esaka
Keyword(s):  
Dna Binding ◽  
Zinc Finger ◽  
Binding Protein ◽  
Dna Binding Protein ◽  
Binding Domain ◽  
Dna Binding Domain ◽  
Functional Analyses

A Nondenaturing Purification Scheme for the DNA-Binding Domain of Poly(ADP-Ribose) Polymerase, a Structure-Specific DNA-Binding Protein

1998 ◽  
Vol 14 (1) ◽  
pp. 79-86 ◽  
Author(s):  
Tracy Ruscetti ◽  
Janet Newman ◽  
Thomas S. Peat ◽  
Jennifer Francis ◽  
Rhiannon Nolan ◽  
...  
Keyword(s):  
Dna Binding ◽  
Binding Protein ◽  
Dna Binding Protein ◽  
Binding Domain ◽  
Dna Binding Domain ◽  
Purification Scheme
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