Bovine viral diarrhoea virus infection in livestock in southern Africa.

Author(s):  
M Vuuren
Author(s):  
Terence P. Scott ◽  
Eleanor Stylianides ◽  
Wanda Markotter ◽  
Louis Nel

Bovine viral diarrhoea virus (BVDV) is a pestivirus that affects members of the order Artiodactyla, including members of the subfamily Bovinae. Little is known about the seroprevalence of BVDV in southern Africa, especially the prevalence in wild ruminant populations such as kudu (Tragelaphus strepsiceros). A handful of random surveys suggested that seroprevalence ranged between 6% and 70% in southern African wild ruminants. The present study aimed to determine the seroprevalence of BVDV amongst kudu and eland (Taurotragus oryx) from Namibia and South Africa. A BVDV-specific enzyme-linked immunosorbent assay was performed on 50 serum samples from kudu and eland from South Africa and Namibia. The seroprevalence of BVDV in South African kudu was 71%, identical to that in Namibian kudu. The seroprevalence in Namibian eland was 40%. The kudu and cattle farming (free ranging) regions in Namibia predominantly overlap in the central regions, ensuring ample opportunity for cross-species transmission of BVDV. It is therefore important to determine the true prevalence of BVDV in southern Africa in both domesticated and wild animals. In addition, a potential link between BVDV incidence and a devastating rabies epidemic in Namibian kudu was proposed and such a notion could be supported or discredited by comparative prevalence data.


2017 ◽  
Vol 220 ◽  
pp. 80-87 ◽  
Author(s):  
Veronika Richter ◽  
Karin Lebl ◽  
Walter Baumgartner ◽  
Walter Obritzhauser ◽  
Annemarie Käsbohrer ◽  
...  

Author(s):  
N. Kabongo ◽  
C. Baule ◽  
M. Van Vuuren

The presence of bovine viral diarrhoea virus in South Africa has been confirmed by several serological surveys. However, little is known about its biological properties. Twenty five isolates obtained by isolation in tissue culture and detected by means of the antigen capture ELISA from clinically sick cattle and from foetal calf serum in South Africa were characterized on the basis of analysis of the 5' non-translated (NTR) region of the genome. A reverse-transcription polymerase chain reaction (RT-PCR) was used to amplify specific sequences from the 5'NTR of the genome. The oligonucleotide primers corresponding to positions 105-125 and 399-378, respectively, in the sequence of BVDV strain NADL were used to generate the PCR products. Both strands were sequenced directly with these primers and fluorescence-labelled dideoxynucleotides in an automated nucleic acid sequencer. Reference strains of pestiviruses [(BVDV type I, BVDV type II, border disease virus (BDV) and hog cholera virus (HCV)] and isolates from a previous investigation on BVDV in southern Africa were included for comparative purposes. All the BVDV strains obtained during this study belong to subgroups of BVDV genotype I. No association could be demonstrated between the geographic origin of the isolates. A number of isolates formed another branch separate from the existing branches Ia, Ib and Ic. These findings suggest that extensive genetic diversity can be found within BVDV type I isolates from southern Africa. Isolates that group with the classical BVDV type I strains, particularly of American origin, coexist with variants that appear to represent a local genetic pool and or variants evolving from the classical strains.


Author(s):  
N. Kabongo ◽  
M. Van Vuuren

Studies covering all aspects of bovine viral diarrhoea virus (BVDV) have been conducted in several countries in Europe, Asia and America. In southern Africa, more information is required about the nature of BVDV infection, the prevalence of different strains and the economic importance of the disease. The presence of BVDV in southern Africa has been known since the early 1970s through serological surveys but few reports confirming its presence by virus isolation and correlation with clinical disease are available. Specimens (n = 312) collected in 1998/99, from live and dead cattle from different farming systems, were obtained from private practitioners, feedlot consultants and abattoirs throughout the country. Specimens (n=37) from African buffaloes (Syncerus caffer) in the Kruger National Park were also included. All specimens were processed for virus isolation in cell culture with confirmation by means of immunofluorescent antibody tests and some also by means of an antigen capture ELISA. BVDV was isolated from 15 (4.7 %) cattle and were all noncytopathic biotypes. BVDV was not detected in 37 lymph nodes obtained from buffaloes in the Kruger National Park. Of the clinical signs in cattle from which virus were isolated, respiratory signs was the most frequent (10/15), followed by diarrhoea (5/15). Abortion, congenital malformations, haemorrhagic diarrhoea and poor growth were also included as criteria for selection of animals for specimen collection, but no BVD viruses were isolated from cattle manifesting these clinical signs.


2007 ◽  
Vol 161 (3) ◽  
pp. 94-99 ◽  
Author(s):  
A. P. Foster ◽  
M. G. Houlihan ◽  
J. P. Holmes ◽  
E. J. Watt ◽  
R. J. Higgins ◽  
...  

2004 ◽  
Vol 99 (1) ◽  
pp. 51-57 ◽  
Author(s):  
Violeta Mockeliūnien≐ ◽  
Algirdas Šalomskas ◽  
Raimundas Mockeliūnas ◽  
Saulius Petkevičius

Author(s):  
Zhangrui Cheng ◽  
Latta Chauhan ◽  
Amy Teresa Barry ◽  
Ayimuguli Abudureyimu ◽  
Chike F Oguejiofor ◽  
...  

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