scholarly journals Serological survey of bovine viral diarrhoea virus in Namibian and South African kudu (Tragelaphus strepsiceros) and eland (Taurotragus oryx)

2013 ◽  
Vol 84 (1) ◽  
Author(s):  
Terence P. Scott ◽  
Eleanor Stylianides ◽  
Wanda Markotter ◽  
Louis Nel
Keyword(s):  
South Africa ◽  
South African ◽  
Southern Africa ◽  
Bovine Viral Diarrhoea Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Bovine Viral Diarrhoea ◽  
Serum Samples ◽  
Diarrhoea Virus ◽  
Central Regions ◽  
Tragelaphus Strepsiceros

Bovine viral diarrhoea virus (BVDV) is a pestivirus that affects members of the order Artiodactyla, including members of the subfamily Bovinae. Little is known about the seroprevalence of BVDV in southern Africa, especially the prevalence in wild ruminant populations such as kudu (Tragelaphus strepsiceros). A handful of random surveys suggested that seroprevalence ranged between 6% and 70% in southern African wild ruminants. The present study aimed to determine the seroprevalence of BVDV amongst kudu and eland (Taurotragus oryx) from Namibia and South Africa. A BVDV-specific enzyme-linked immunosorbent assay was performed on 50 serum samples from kudu and eland from South Africa and Namibia. The seroprevalence of BVDV in South African kudu was 71%, identical to that in Namibian kudu. The seroprevalence in Namibian eland was 40%. The kudu and cattle farming (free ranging) regions in Namibia predominantly overlap in the central regions, ensuring ample opportunity for cross-species transmission of BVDV. It is therefore important to determine the true prevalence of BVDV in southern Africa in both domesticated and wild animals. In addition, a potential link between BVDV incidence and a devastating rabies epidemic in Namibian kudu was proposed and such a notion could be supported or discredited by comparative prevalence data.

scholarly journals Molecular analysis of bovine viral diarrhoea virus isolates from South Africa

2003 ◽  
Vol 70 (4) ◽  
Author(s):  
N. Kabongo ◽  
C. Baule ◽  
M. Van Vuuren
Keyword(s):  
South Africa ◽  
Southern Africa ◽  
Calf Serum ◽  
Bovine Viral Diarrhoea Virus ◽  
Border Disease Virus ◽  
Foetal Calf Serum ◽  
Type I ◽  
Bovine Viral Diarrhoea ◽  
Border Disease ◽  
Diarrhoea Virus

The presence of bovine viral diarrhoea virus in South Africa has been confirmed by several serological surveys. However, little is known about its biological properties. Twenty five isolates obtained by isolation in tissue culture and detected by means of the antigen capture ELISA from clinically sick cattle and from foetal calf serum in South Africa were characterized on the basis of analysis of the 5' non-translated (NTR) region of the genome. A reverse-transcription polymerase chain reaction (RT-PCR) was used to amplify specific sequences from the 5'NTR of the genome. The oligonucleotide primers corresponding to positions 105-125 and 399-378, respectively, in the sequence of BVDV strain NADL were used to generate the PCR products. Both strands were sequenced directly with these primers and fluorescence-labelled dideoxynucleotides in an automated nucleic acid sequencer. Reference strains of pestiviruses [(BVDV type I, BVDV type II, border disease virus (BDV) and hog cholera virus (HCV)] and isolates from a previous investigation on BVDV in southern Africa were included for comparative purposes. All the BVDV strains obtained during this study belong to subgroups of BVDV genotype I. No association could be demonstrated between the geographic origin of the isolates. A number of isolates formed another branch separate from the existing branches Ia, Ib and Ic. These findings suggest that extensive genetic diversity can be found within BVDV type I isolates from southern Africa. Isolates that group with the classical BVDV type I strains, particularly of American origin, coexist with variants that appear to represent a local genetic pool and or variants evolving from the classical strains.

A survey for bvdv antibodies in cattle farms in Slovakia and genetic typing of bvdv isolates from imported animals

2003 ◽  
Vol 51 (2) ◽  
pp. 229-236 ◽  
Author(s):  
Š. Vilček ◽  
Jana Mojžišová ◽  
Viera Bajová ◽  
Š. Paulík ◽  
L. Strojný ◽  
...  
Keyword(s):  
Bovine Viral Diarrhoea Virus ◽  
Computer Assisted ◽  
Serological Survey ◽  
Bovine Viral Diarrhoea ◽  
Rt Pcr ◽  
Serum Samples ◽  
Genetic Typing ◽  
Diarrhoea Virus ◽  
Pcr Products ◽  
Cattle Farms

A serological survey for bovine viral diarrhoea virus (BVDV) antibodies on a collection of 1295 serum samples obtained from 6-12 months old cattle originating from 45 farms in Slovakia was carried out. On 13 farms more than 90% of the examined animals were seropositive, on 14 farms 71-90% seroprevalence was observed, on 13 farms only 50-70% animals were found to be positive for BVDV antibodies, while the remaining 5 farms showed fewer than 50% seropositive animals. The average incidence of BVDV antibodies (around 70%) was similar as determined 30 years ago. Of 84 serum samples from seronegative animals originating from 14 farms in which 70-98% seropositivity was observed, six were positive in Ag-BVDV ELISA indicating persistently infected (PI) cattle. On a farm to which animals were imported from abroad, a BVD outbreak was observed. Of 110 animals tested, four were positive in Ag-ELISA indicating the presence of PI cattle on this farm. Genetic typing of two isolates from imported animals performed by RT-PCR (324/326 primers from 5´-UTR), sequencing of PCR products and computer-assisted phylogenetic analysis revealed that they belong to BVDV-1h group.

scholarly journals Detection of bovine viral diarrhoea virus in specimens from cattle in South Africa and possible association with clinical disease

2004 ◽  
Vol 75 (2) ◽  
Author(s):  
N. Kabongo ◽  
M. Van Vuuren
Keyword(s):  
Southern Africa ◽  
National Park ◽  
Virus Isolation ◽  
Clinical Signs ◽  
Bovine Viral Diarrhoea Virus ◽  
Kruger National Park ◽  
Clinical Disease ◽  
Bovine Viral Diarrhoea ◽  
Poor Growth ◽  
Diarrhoea Virus

Studies covering all aspects of bovine viral diarrhoea virus (BVDV) have been conducted in several countries in Europe, Asia and America. In southern Africa, more information is required about the nature of BVDV infection, the prevalence of different strains and the economic importance of the disease. The presence of BVDV in southern Africa has been known since the early 1970s through serological surveys but few reports confirming its presence by virus isolation and correlation with clinical disease are available. Specimens (n = 312) collected in 1998/99, from live and dead cattle from different farming systems, were obtained from private practitioners, feedlot consultants and abattoirs throughout the country. Specimens (n=37) from African buffaloes (Syncerus caffer) in the Kruger National Park were also included. All specimens were processed for virus isolation in cell culture with confirmation by means of immunofluorescent antibody tests and some also by means of an antigen capture ELISA. BVDV was isolated from 15 (4.7 %) cattle and were all noncytopathic biotypes. BVDV was not detected in 37 lymph nodes obtained from buffaloes in the Kruger National Park. Of the clinical signs in cattle from which virus were isolated, respiratory signs was the most frequent (10/15), followed by diarrhoea (5/15). Abortion, congenital malformations, haemorrhagic diarrhoea and poor growth were also included as criteria for selection of animals for specimen collection, but no BVD viruses were isolated from cattle manifesting these clinical signs.

scholarly journals Genotyping of bovine viral diarrhoea virus isolates from the Czech Republic

2009 ◽  
Vol 54 (No. 9) ◽  
pp. 393-398 ◽  
Author(s):  
B. Robesova ◽  
K. Kovarcik ◽  
S. Vilcek
Keyword(s):  
Czech Republic ◽  
Bovine Viral Diarrhoea Virus ◽  
Computer Assisted ◽  
Bovine Viral Diarrhoea ◽  
Rt Pcr ◽  
Serum Samples ◽  
The Czech Republic ◽  
Genetic Typing ◽  
Diarrhoea Virus ◽  
Virus Isolates

This study was focused on the genetic typing of bovine viral diarrhoea virus (BVDV) isolates obtained from 41 serum samples of persistently infected cattle in the Czech Republic in the period of 2004 to 2007. For the differentiation of BVDV isolates, the 5’-UTR and Npro</sup> regions were selected. A 288-bp fragment from 5’-UTR and 428-bp fragment from Npro of the selected isolates were amplified by RT-PCR and subsequently sequenced and analysed by computer-assisted phylogenetic analysis. The isolates belong to BVDV-1 genotype and the following subtypes were identified: b (<i>n</i> = 16), d (<i>n</i> = 16), e (<i>n</i> = 2) and f (<i>n</i> = 7). In this collection of viral samples, no isolate belonged to BVDV-2 genotype.

scholarly journals Serological survey of wild cervids in England and Wales for bovine viral diarrhoea virus

2020 ◽  
Vol 187 (7) ◽  
pp. e47-e47
Author(s):  
Jo Hardstaff ◽  
Hannah Hunt ◽  
Laura Tugwell ◽  
Carole Thomas ◽  
Laila Elattar ◽  
...  
Keyword(s):  
Bovine Viral Diarrhoea Virus ◽  
The Other ◽  
Bovine Viral Diarrhoea ◽  
Serum Samples ◽  
East Anglia ◽  
England And Wales ◽  
Source Of Infection ◽  
Diarrhoea Virus ◽  
Cattle Herds ◽  
Wild Deer

BackgroundBovine viral diarrhoea (BVD) is a production disease commonly found in British cattle herds. Species other than cattle have been shown to be infected with the virus, thereby providing a potential source of infection for livestock. This study surveyed serum samples taken from 596 culled wild deer from England and Wales, between 2009 and 2010, for the presence of BVD antibodies.Methods596 samples were tested with the SVANOVIR BVDV p80-Ab ELISA and a subset of 64 were tested with the IDEXX BVDV p80-Ab ELISA. ELISA results were confirmed using serum neutralisation tests.Results2/596 samples (0.35 per cent) tested positive for BVD antibodies using the Svanova test and one of these tested positive and the other inconclusive using the IDEXX test; both were confirmed positive with serum neutralisation tests. These were both red deer stags, one from Devon and the other from East Anglia.ConclusionsThe results indicate that it is unlikely that BVD virus is widely circulating within the wild deer population and particularly unlikely that persistently infected deer are present in the populations surveyed. These results suggest that wild deer are unlikely to be a significant reservoir of BVD infection in cattle.

scholarly journals Evaluation of diagnostic methods to distinguish between calves persistently and transiently infected with bovine viral diarrhoea virus in respect to the presence of maternal antibodies

2013 ◽  
Vol 57 (3) ◽  
pp. 311-317
Author(s):  
Magdalena Larska ◽  
Aleksandra Kuta ◽  
Mirosław P. Polak
Keyword(s):  
Virus Detection ◽  
Bovine Viral Diarrhoea Virus ◽  
Diagnostic Methods ◽  
Viral Rna ◽  
Maternal Antibodies ◽  
Bovine Viral Diarrhoea ◽  
Rt Pcr ◽  
Serum Samples ◽  
Virus Genotype ◽  
Diarrhoea Virus

Abstract Two issues concerning virus detection and identification of persistently infected (PI) cattle were analysed in the study: 1) interference by maternal antibodies and 2) discrimination between PI and transiently infected (TI) animals. Antigen ELISA and RT-PCR based methods were compared using serum samples from natural and experimental PI and TI calves. RT-PCR and realtime RT-PCR using primers within 5’UTR region were more sensitive in detecting PI animals than Erns and NS3 antigen capture ELISAs, and they were not influenced by the presence of colostral antibodies in serum or by bovine viral diarrhoea virus genotype. The serum samples with Ct values ≤ 29.10 (corresponding to 104.87 viral RNA copies/μL) identified PI animals with 100% probability, while all samples with Ct values > 32.06 (corresponding to viral RNA load below 104 copies/μL) indicated TI status. The samples with Ct values between 29.10 and 32.06 (17.2% of PI and 11.5% of TI) should be considered as PI suspect and retested.

Natural transmission of bovine viral diarrhoea virus-1c from a persistently infected neonate lamb to naïve sheep and cattle

2018 ◽  
Vol 182 (12) ◽  
pp. 352-352 ◽  
Author(s):  
Caitlin Alexandra Evans ◽  
Farhid Hemmatzadeh ◽  
Michael P Reichel ◽  
Peter D Cockcroft
Keyword(s):  
Viral Load ◽  
Quantitative Pcr ◽  
Bovine Viral Diarrhoea Virus ◽  
Bovine Viral Diarrhoea ◽  
Serum Samples ◽  
Specific Antibodies ◽  
Persistently Infected ◽  
Treatment Groups ◽  
Viral Loads ◽  
Diarrhoea Virus

This study investigated the transmission of bovine viral diarrhoea virus (BVDV)-1c from a persistently infected (PI) neonate lamb to naïve sheep and cattle using three treatment groups: four naïve ewes and their five lambs, which were copaddocked with the PI lamb; five steers, which were housed in a paddock adjacent to the PI lamb; and five steers, which had direct but limited exposure to the PI lamb. Serum samples were collected and tested for BVDV-specific antibodies. Serum samples from the PI lamb, from day of birth to eight weeks of age, were tested for BVDV-specific antibodies and antigen and submitted for quantitative PCR to determine the viral load present at each week of age. Only one lamb from the copaddocked group developed BVDV-specific antibodies following comingling while all the steers in both the cattle treatment groups remained BVDV antibody negative. Quantitative PCR results from the PI lamb showed lower viral loads from day of birth to six weeks of age, compared with the results at seven and eight weeks of age. This may reflect maternal colostral BVDV antibody concentrations in the neonate lamb or other viral properties.

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