Effect of Ginger Extract on Liver Damage in Experimental Obstructive Jaundice Produced by Main Bile Duct Ligation

Purpose: Reversible obstructive jaundice models have some limiting features, including the need for a second anaesthesia, re-laparotomy and surgical intervention after common bile duct ligation. The present study investigates the feasibility of a new application that can eliminate these limitations. Rapidly absorbable suture materials were used for ligation; therefore, spontaneous biliary decompression was anticipated by the self release of these rapidly degrading materials. Methods: Common bile ducts in Wistar Albino rats were ligated with silk, polyglytone 6211, or irradiated polyglactine 910 (n=7 for each group). Rats were grouped according to both the suture materials and the experiments termination date: 5 days (sham, silk5, polyglytone5, polyglactine5) and 21 days (silk21, polyglytone21, polyglactine21) after the ligation. Biochemical and morphologic changes of liver were assessed. Results: The group polyglactine21 showed significantly lower mean ALT, AST, GGT, total and direct bilirubin values when compared with the group polyglactine5 (p=0.004-0.037). Morphologic changes did not correlate with the biochemical amelioration. In the group polyglytone21, not only the biochemical but also the morphologic changes significantly ameliorated when compared with the group polyglytone5 (p=0.003-0.043). No procedure associated mortality was observed. Conclusion: Common bile duct ligation with polyglytone offers a new reversible model for prolonged obstructive jaundice which abolishes the need for relaparotomy and a second surgical intervention and significantly reduces mortality.

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Cell-specific regulatory effects of CXCR2 on cholestatic liver injury

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.00080.2019 ◽

2019 ◽

Vol 317 (6) ◽

pp. G773-G783 ◽

Cited By ~ 2

Author(s):

Takanori Konishi ◽

Rebecca M. Schuster ◽

Holly S. Goetzman ◽

Charles C. Caldwell ◽

Alex B. Lentsch

Keyword(s):

Bile Duct ◽

Liver Injury ◽

Liver Damage ◽

Chemokine Receptor ◽

Therapeutic Target ◽

Bile Duct Ligation ◽

Wild Type ◽

Neutrophil Accumulation ◽

Duct Ligation ◽

Cholestatic Liver Injury

The CXC chemokine receptor 2 (CXCR2) is critical for neutrophil recruitment and hepatocellular viability but has not been studied in the context of cholestatic liver injury following bile duct ligation (BDL). The present study sought to elucidate the cell-specific roles of CXCR2 on acute liver injury after BDL. Wild-type and CXCR2−/− mice were subjected BDL. CXCR2 chimeric mice were created to assess the cell-specific role of CXCR2 on liver injury after BDL. SB225002, a selective CXCR2 antagonist, was administrated intraperitoneally after BDL to investigate the potential of pharmacological inhibition. CXCR2−/− mice had significantly less liver injury than wild-type mice at 3 and 14 days after BDL. There was no difference in biliary fibrosis among groups. The chemokines CXCL1 and CXCL2 were induced around areas of necrosis and biliary structures, respectively, both areas where neutrophils accumulated after BDL. CXCR2−/− mice showed significantly less neutrophil accumulation in those injured areas. CXCR2Liver+/Myeloid+ and CXCR2Liver−/Myeloid− mice recapitulated the wild-type and CXCR2-knockout phenotypes, respectively. CXCR2Liver+/Myeloid+ mice suffered higher liver injury than CXCR2Liver+/Myeloid− and CXCR2Liver−/Myeloid+; however, only those chimeras with knockout of myeloid CXCR2 (CXCR2Liver+/Myeloid− and CXCR2Liver−/Myeloid−) showed reduction of neutrophil accumulation around areas of necrosis. Daily administration of SB225002 starting after 3 days of BDL reduced established liver injury at 6 days. In conclusion, neutrophil CXCR2 guides the cell to the site of injury, while CXCR2 on liver cells affects liver damage independent of neutrophil accumulation. CXCR2 appears to be a viable therapeutic target for cholestatic liver injury. NEW & NOTEWORTHY This study is the first to reveal cell-specific roles of the chemokine receptor CXCR2 in cholestatic liver injury caused by bile duct ligation. CXCR2 on neutrophils facilitates neutrophil recruitment to the liver, while CXCR2 on liver cells contributes to liver damage independent of neutrophils. CXCR2 may represent a viable therapeutic target for cholestatic liver injury.

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Effect of obstructive jaundice on the regulation of hepatic cholesterol metabolism in the rat Disappearance of abcg5 and abcg8 mRNA after bile duct ligation

Hepatology Research ◽

10.1016/s1386-6346(02)00248-6 ◽

2003 ◽

Vol 25 (2) ◽

pp. 99-104 ◽

Cited By ~ 14

Author(s):

T Kamisako

Keyword(s):

Bile Duct ◽

Obstructive Jaundice ◽

Bile Duct Ligation ◽

Cholesterol Metabolism ◽

Hepatic Cholesterol ◽

Duct Ligation

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Inhibition of the biosynthesis of uroporphyrinogen and heme in rat liver during obstructive jaundice produced by bile duct ligation

Archives of Biochemistry and Biophysics ◽

10.1016/0003-9861(86)90457-1 ◽

1986 ◽

Vol 246 (1) ◽

pp. 143-148 ◽

Cited By ~ 2

Author(s):

Walter N. Piper ◽

James Tse ◽

Emily M. Sadler ◽

W.Russ Christenson ◽

James L. Balk ◽

...

Keyword(s):

Bile Duct ◽

Obstructive Jaundice ◽

Rat Liver ◽

Bile Duct Ligation ◽

Duct Ligation

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Renal dysfunction as a consequence of acute liver damage by bile duct ligation in cirrhotic rats

Experimental and Toxicologic Pathology ◽

10.1016/j.etp.2006.05.001 ◽

2006 ◽

Vol 58 (2-3) ◽

pp. 185-195 ◽

Cited By ~ 24

Author(s):

Sandra Rivera-Huizar ◽

Ana Rosa Rincón-Sánchez ◽

Amador Covarrubias-Pinedo ◽

María Cristina Islas-Carbajal ◽

Genaro Gabriel-Ortíz ◽

...

Keyword(s):

Bile Duct ◽

Renal Dysfunction ◽

Liver Damage ◽

Bile Duct Ligation ◽

Acute Liver Damage ◽

Duct Ligation

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Increased Cardiac Endocrine Activity After Common Bile Duct Ligation in the Rabbit Atrial Endocrine Cells in Obstructive Jaundice

Annals of Surgery ◽

10.1097/00000658-199401000-00012 ◽

1994 ◽

Vol 219 (1) ◽

pp. 73-78 ◽

Cited By ~ 10

Author(s):

J. A. Pereira ◽

M. A. Torregrosa ◽

F. Martínez-Ródenas ◽

J. Clàrla ◽

L. Pallarés ◽

...

Keyword(s):

Bile Duct ◽

Common Bile Duct ◽

Obstructive Jaundice ◽

Endocrine Cells ◽

Bile Duct Ligation ◽

Common Bile Duct Ligation ◽

Endocrine Activity ◽

Duct Ligation

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Comparing distress of mouse models for liver damage

Scientific Reports ◽

10.1038/s41598-020-76391-w ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Guanglin Tang ◽

Nico Seume ◽

Christine Häger ◽

Simone Kumstel ◽

Kerstin Abshagen ◽

...

Keyword(s):

Multivariate Analysis ◽

Carbon Tetrachloride ◽

Bile Duct ◽

Animal Models ◽

Mouse Models ◽

Liver Damage ◽

Bile Duct Ligation ◽

Characteristic Curve ◽

Support Vector ◽

Duct Ligation

AbstractIn order to foster animal welfare as well as high quality of research, many countries regulate by law that the severity of animal experiments must be evaluated and considered when performing biomedical research. It is well accepted that multiple parameters rather than a single readout parameter should be applied to describe animal distress or suffering. However, since the performance of readout parameters for animal distress is rarely defined and methods for multivariate analysis have only in rare cases been used, it is not known which methodology is most appropriate to define animal distress. This study used receiver operating characteristic curve analysis to quantify the performance of burrowing activity, body weight change and a distress score of mice after induction of liver damage by bile duct ligation or carbon tetrachloride. In addition, Support Vector Machine classification was used to compare the distress of these mouse models. This approach demonstrated that bile duct ligation causes much more distress than carbon tetrachloride-induced liver damage. This study, therefore, provides a prototype how to compare two animal models by considering several readout parameters. In the future these or similar methods for multivariate analysis will be necessary, when assessing and comparing the severity of animal models.

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