Purification and Characterization of Tissue Plasminogen Activator Secreted by Human Embryonic Lung Diploid Fibroblasts, IMR-90 Cells

1991 ◽  
Vol 55 (5) ◽  
pp. 1225-1232
Author(s):  
Yasuharu Itagaki ◽  
Hisataka Yasuda ◽  
Tomonori Morinaga ◽  
Shinjiro Mitsuda ◽  
Kanji Higashio
Keyword(s):  
Plasminogen Activator ◽  
Tissue Plasminogen Activator ◽  
Embryonic Lung ◽  
Purification And Characterization ◽  
Human Embryonic Lung ◽  
Diploid Fibroblasts ◽  
Tissue Plasminogen

scholarly journals The glycosylation of Bowes melanoma tissue plasminogen activator: lectin mapping, reaction with anti-L2/HNK-1 antibodies and the presence of sulphated/glucuronic acid containing glycans

1996 ◽  
Vol 316 (2) ◽  
pp. 427-437 ◽  
Author(s):  
A. J. JAQUES ◽  
G. OPDENAKKER ◽  
T. W. RADEMACHER ◽  
R. A. DWEK ◽  
S. E. ZAMZE
Keyword(s):  
Cell Line ◽  
Plasminogen Activator ◽  
Tissue Plasminogen Activator ◽  
Glucuronic Acid ◽  
Gel Filtration ◽  
Lectin Affinity Chromatography ◽  
Tissue Plasminogen ◽  
A Cell ◽  
Neuroectodermal Origin

The glycosylation of tissue plasminogen activator (t-PA) obtained from the Bowes melanoma cell line was re-examined using methods of serial lectin affinity chromatography coupled with Bio-Gel P-4 gel filtration chromatography and exoglycosidase sequencing. This study clarified an earlier discrepancy in the literature and confirmed that the major complex N-linked glycans on Bowes t-PA that carry sialic acid as their sole charged group are bi-antennary, core fucosylated, with terminal N-acetylgalactosamine residues. We also report the characterization of a series of related and previously unidentified sialylated glycans. Further we show that Bowes t-PA expresses glucuronic acid/sulphate containing N-linked glycans and is recognized by anti-carbohydrate L2/HNK-1 monoclonal antibodies. The presence on Bowes t-PA of glycans associated primarily with the nervous system is consistent with its expression in a cell line of neuroectodermal origin.

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