Synthetic Cannabinoids 5F-QUPIC and MDMB-CHMICA in Plant Material – Identification and Quantification by Gas Chromatography – Mass Spectrometry (GC-MS), Nuclear Magnetic Resonance (NMR), and High-Performance Liquid Chromatography with Diode Array Detection (HPLC-DAD)

2021 ◽  
pp. 1-11
Author(s):  
Ivo D. Ivanov ◽  
Silviya S. Stoykova ◽  
Nikola T. Burdzhiev ◽  
Ivayla N. Pantcheva ◽  
Vasil N. Atanasov
Keyword(s):  
Mass Spectrometry ◽  
Nuclear Magnetic Resonance ◽  
Gas Chromatography ◽  
Plant Material ◽  
High Performance ◽  
Synthetic Cannabinoids ◽  
Gas Chromatography Mass Spectrometry ◽  
Diode Array ◽  
Diode Array Detection ◽  
Array Detection

scholarly journals Tetrahydropalmatine Poisoning: Diagnoses of Nine Adult Overdoses Based on Toxicology Screens by HPLC with Diode-Array Detection and Gas Chromatography–Mass Spectrometry

1999 ◽  
Vol 45 (2) ◽  
pp. 229-236 ◽  
Author(s):  
Chi-Kong Lai ◽  
Albert Yan-Wo Chan
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Solid Phase ◽  
Gas Chromatography Mass Spectrometry ◽  
Diode Array ◽  
Diode Array Detection ◽  
Chromatography Mass Spectrometry ◽  
Polar Metabolites ◽  
Array Detection ◽  
Neurological Disturbance

Abstract Background: Tetrahydropalmatine (THP) is a neuroactive alkaloid with analgesic and hypnotic action. Its analysis is important because cases of human poisonings have emerged as a result of unregulated use of some proprietary biopharmaceuticals containing purified THP. Methods: We established analytical parameters for HPLC with diode-array detection (HPLC-DAD) and gas chromatography–mass spectrometry (GC-MS) for the detection of THP in serum and urine. Nine acutely THP-poisoned adults were thus screened over 16 months. Results: All patients recovered quickly after mild neurological disturbance. In general, THP was metabolized rapidly and excreted as polar metabolites in urine. Serum THP was measured in five cases and found to be <0.1–1.2 mg/L (<0.3–3.4 μmol/L). Paired analyses of urine with and without glucuronidase treatment clarified the disposition of THP. Our GC–MS method with trimethylsilane derivatization identified O-desmethyl metabolites. With a uniform solid-phase extraction, the HPLC-DAD procedure detected intact glucuronide metabolites. Conclusion: Intact glucuronide metabolites of THP are sensitive markers for THP exposures. Our methods and findings provide practical tools and information for surveillance of intoxication caused by excessive THP intake.

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