Evans Blue Staining Method for Detection of Proteins on Polyacrylamide Gels with Rhodamine B

1994 ◽  
Vol 27 (7) ◽  
pp. 1265-1275 ◽  
Author(s):  
Do-Seong Na ◽  
Hee-Youn Hong ◽  
Gyurng-Soo Yoo ◽  
Jung-Kap Choi
Keyword(s):  
Rhodamine B ◽  
Evans Blue ◽  
Staining Method ◽  
Blue Staining ◽  
Polyacrylamide Gels

scholarly journals An Improved Evans Blue Staining Method for Consistent, Accurate Assessment of Plasmodiophora brassicae Resting Spore Viability

Plant Disease ◽  
2019 ◽  
Vol 103 (9) ◽  
pp. 2330-2336 ◽  
Author(s):  
M. W. Harding ◽  
T. B. Hill ◽  
Y. Yang ◽  
G. C. Daniels ◽  
S. F. Hwang ◽  
...  
Keyword(s):  
Evans Blue ◽  
Staining Method ◽  
Plasmodiophora Brassicae ◽  
Blue Staining ◽  
Inoculum Potential ◽  
Spore Viability ◽  
Modified Method ◽  
Resting Spore ◽  
Resting Spores ◽  
Vital Stains

Clubroot caused by Plasmodiophora brassicae is an important disease of brassica crops. The use of vital stains to determine the viability of P. brassicae resting spores can provide useful information regarding spore longevity, inoculum potential, or the efficacy of antimicrobial treatments. Evans blue is one example of a vital stain that has been reported to differentially stain viable and nonviable resting spores. Some previously published protocols using Evans blue to stain P. brassicae resting spores have not provided accurate or consistent results. In this study, we modified the Evans blue method by increasing the staining time to 8 h or more and evaluated P. brassicae resting spores after heat treatment at various combinations of temperature and time. Extending staining times significantly increased the numbers of stained resting spores up to 7 h, after which the numbers of stained spores did not change significantly (R2 = 96.88; P ≤ 0.001). The accuracy of the modified method to discriminate viable and nonviable spores was evaluated in repeated experiments and by comparing the staining data with those derived from inoculation assays and propidium monoazide quantitative PCR (qPCR). The results demonstrated that the modified Evans blue staining method improved the accuracy and consistency of measurement of P. brassicae resting spore viability. Additionally, it was equivalent to the qPCR method for differentiating viable and nonviable spores (R2 = 99.84; P ≤ 0.001) and confirmed in canola infection bioassays.

Regional blood flow and capillary permeability in the ethylnitrosourea-induced rat glioma

1981 ◽  
Vol 55 (6) ◽  
pp. 922-928 ◽  
Author(s):  
Kazuo Yamada ◽  
Toru Hayakawa ◽  
Yukitaka Ushio ◽  
Norio Arita ◽  
Amami Kato ◽  
...  
Keyword(s):  
Blood Flow ◽  
Brain Tissue ◽  
Evans Blue ◽  
Capillary Permeability ◽  
Regional Blood Flow ◽  
Blue Staining ◽  
Content Type ◽  
Rat Glioma ◽  
Blood Flows ◽  
Diameter Reduction

✓ Regional cerebral blood flow and capillary permeability of rat brains bearing ethylnitrosourea-induced gliomas of various size were investigated with 14C-antipyrine autoradiography and Evans blue staining. In the small tumors (<2 mm in diameter), blood flow was uniformly reduced when compared to the adjacent brain. Even in tiny tumors (0.3 to 0.4 mm in diameter), reduction in blood flow was evident. In the medium (2 to 4 mm in diameter) and large (> 4 mm in diameter) tumors, the blood flow increased or decreased depending on the part of the tumor examined. The necrotic center and peripheral edge had low blood flows, whereas the viable portion adjacent to the necrotic center had high blood flows. Blood flow in the brain tissue adjacent to medium and large tumors was lower than control brain tissue, probably due to local edema. Leakage of intravenous Evans blue in the tissue was only evident in the large tumors with central necrosis. The present findings suggest that neovascularization of the tumor may occur when the tumor reaches a certain size, and leaky new vessels may be the cause of brain edema associated with tumor.

Application of the Methylene Blue Staining Method to the Study of Experimentally PNNG-Induced Intestinal Metaplasia of the Canine Stomach

Endoscopy ◽  
1980 ◽  
Vol 12 (05) ◽  
pp. 205-210 ◽  
Author(s):  
T. Hashimoto ◽  
S. Suzuku ◽  
T. Hasegawa ◽  
N. Sakakibara ◽  
M. Endo ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Intestinal Metaplasia ◽  
Staining Method ◽  
Blue Staining

scholarly journals Identification of Chrysanthemum (Chrysanthemum morifolium) Self-Incompatibility

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Fan Wang ◽  
Feng-Jiao Zhang ◽  
Fa-Di Chen ◽  
Wei-Min Fang ◽  
Nian-Jun Teng
Keyword(s):  
Small Proportion ◽  
Seed Set ◽  
Staining Method ◽  
Pollen Grains ◽  
Chrysanthemum Morifolium ◽  
Fluorescence Microscope ◽  
Blue Staining ◽  
Self Incompatibility ◽  
Wide Range ◽  
Aniline Blue Staining

There has been a heated argument over self-incompatibilityof chrysanthemum (Chrysanthemum morifolium) among chrysanthemum breeders. In order to solve the argument, we investigated pistil receptivity, seed set, and compatible index of 24 chrysanthemum cultivars. It was found that the 24 cultivars averagely had 3.7–36.3 pollen grains germinating on stigmas at 24 hours after self-pollination through the fluorescence microscope using aniline blue staining method. However, only 10 of them produced self-pollinated seeds, and their seed sets and compatible indexes were 0.03–56.50% and 0.04–87.50, respectively. The cultivar “Q10-33-1” had the highest seed set (56.50%) and compatible index (87.50), but ten of its progeny had a wide range of separation in seed set (0–37.23%) and compatible index (0–68.65). The results indicated that most of chrysanthemum cultivars were self-incompatible, while a small proportion of cultivars were self-compatible. In addition, there is a comprehensive separation of self-incompatibility among progeny from the same self-pollinated self-compatible chrysanthemum cultivar. Therefore, it is better to emasculate inflorescences during chrysanthemum hybridization breeding when no information concerning its self-incompatibility characteristics is available. However, if it is self-incompatible and propagated by vegetative methods, it is unnecessary to carry out emasculation when it is used as a female plant during hybridization breeding.

Fluorescent staining of glycoproteins in sodium dodecyl sulfate polyacrylamide gels by 4H-[1]-benzopyrano[4,3-b]thiophene-2-carboxylic acid hydrazide

The Analyst ◽  
2014 ◽  
Vol 139 (11) ◽  
pp. 2764-2773 ◽  
Author(s):  
Zhongxin Zhu ◽  
Xuan Zhou ◽  
Yang Wang ◽  
Lisha Chi ◽  
Dandan Ruan ◽  
...  
Keyword(s):  
Carboxylic Acid ◽  
Sodium Dodecyl Sulfate ◽  
Staining Method ◽  
Sodium Dodecyl ◽  
Acid Hydrazide ◽  
Polyacrylamide Gels ◽  
Fluorescent Staining ◽  
Affinity Enrichment ◽  
Carboxylic Acid Hydrazide ◽  
Sds Page

A sensitive, specific, economic and MS compatible staining method for gel-separated glycoproteins by using BH was described and demonstrated by 1-D and 2-D SDS-PAGE, deglycosylation, glycoprotein affinity enrichment and LC-MS/MS, respectively.

scholarly journals Detection in milk of a 60,000 Mr mouse and a 68,000 Mr human phosphorylated glycoprotein by histochemical analysis on polyacrylamide gels.

1983 ◽  
Vol 31 (6) ◽  
pp. 709-716 ◽  
Author(s):  
M R Green
Keyword(s):  
Molecular Weight ◽  
Sialic Acid ◽  
Periodic Acid ◽  
Sodium Dodecyl ◽  
Apparent Molecular Weight ◽  
Blue Staining ◽  
Polyacrylamide Gels ◽  
Periodic Acid Schiff ◽  
Coomassie Blue Staining ◽  
Coomassie Blue

Proteins in colostrum and skimmed milk from humans and mice were separated by electrophoresis on polyacrylamide gels and stained with Coomassie blue (CB), Ethyl-Stains-all (ESA), and periodic acid-Schiff (PAS) to investigate changes that may occur in milks throughout lactation. In mouse colostrum but not in mature mouse milk, a PAS-positive protein of apparent molecular weight of 60,000 stained prominently blue with ESA. A protein in human milk with a molecular weight of 68,000 stained similarly but was present throughout lactation. The intensity of blue staining of these minor proteins in milk approached that obtained with casein phosphoproteins. The metachromatic dye ESA stains phosphoproteins and sialic acid-rich glycoproteins blue to blue-green. Removal of phosphorus from the former and sialic acid from the latter results in those proteins staining red with ESA. The intensity of blue staining of the 60,000 and 68,000 Mr proteins was diminished but not lost following treatment with phosphatase. It was eliminated following neuraminidase digestion of the mouse protein and mild acid hydrolysis of the human protein. Coomassie blue staining of the proteins was not affected by these procedures. Following electrophoresis of milk and milk fractions in a non-sodium dodecyl sulfate-containing system, the proteins were identified by their characteristic staining properties with ESA and isolated.

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