Spore Germination and Germ Tube Growth of a Vesicular-Arbuscular Mycorrhizal Fungusin Vitro

Mycologia ◽  
1982 ◽  
Vol 74 (6) ◽  
pp. 952-959 ◽  
Author(s):  
J. O. Siqueira ◽  
D. H. Hubbell ◽  
N. C. Schenck
Keyword(s):  
Spore Germination ◽  
Germ Tube ◽  
Arbuscular Mycorrhizal ◽  
Tube Growth ◽  
Vesicular Arbuscular ◽  
Germ Tube Growth

scholarly journals In vitro effect of substrate, temperature and photoperiod on Phakopsora pachyrhizi urediniospore germination and germ tube growth

2015 ◽  
Vol 41 (2) ◽  
pp. 101-106
Author(s):  
Marta Maria Casa Blum ◽  
Erlei Melo Reis ◽  
Francieli Tavares Vieira ◽  
Rita Carlini
Keyword(s):  
Substrate Temperature ◽  
Spore Germination ◽  
Leaf Extract ◽  
Germ Tube ◽  
Tube Growth ◽  
Tube Length ◽  
Germ Tube Length ◽  
Soybean Leaf ◽  
Germ Tube Growth

In vitro experiments were conducted to assess the effects of substrate, temperature and time of exposure to temperature and photoperiod on P. pachyrhizi uredospore germination and germ tube growth. The following substrates were tested: water-agar and soybean leaf extract-agar at different leaf concentrations (0.5, 1.0, 2.0 and 4.0 g of leaves and 15g agar/L water), temperatures (10, 15, 20, 25, 30, and 35oC) and times of exposure (1, 2, 3, 4, 5, 6, 7, and 8 hours) to temperature and 12 different photoperiods. The highest germination and germ tube length was found for the soybean leaf extract agar. Maximum P. pachyrhizi uredospore germination was obtained at 21.8 and 22.3°C, and maximum germ tube growth at 21.4 and 22.1°C. The maximum uredospore germination was found at 6.4 hours exposure, while the maximum germ tube length was obtained at 7.7 h exposure. Regarding photoperiod, the maximum spore germination and the maximum uredospore germ tube length were found in the dark. Neither spore germination nor uredospore germ tube growth was completely inhibited by the exposure to continuous light.

scholarly journals Osmotin and Thaumatin from Grape: A Putative General Defense Mechanism Against Pathogenic Fungi

2003 ◽  
Vol 93 (12) ◽  
pp. 1505-1512 ◽  
Author(s):  
Sara Monteiro ◽  
Mahmoud Barakat ◽  
Maria A. Piçarra-Pereira ◽  
Artur R. Teixeira ◽  
Ricardo B. Ferreira
Keyword(s):  
Botrytis Cinerea ◽  
Spore Germination ◽  
Germ Tube ◽  
Defense Mechanism ◽  
Pr Proteins ◽  
Tube Growth ◽  
Immunological Methods ◽  
Phomopsis Viticola ◽  
Germ Tube Growth

Little information is available concerning the expression of pathogenesis-related (PR) proteins in grapevine (Vitis vinifera) and their effect properties on the major fungal pathogens of grape. A systematic study was performed on the effect of total or individual grape proteins on mycelial growth, spore germination, and germ tube growth of Uncinula necator, Phomopsis viticola, and Botrytis cinerea. Two proteins, identified as PR proteins by immunological methods and by N-terminal sequencing as osmotin and thaumatin-like protein, exhibited strong antifungal activities in vitro, blocking the growth of Phomopsis viticola and Botrytis cinerea mycelia. In addition, they inhibited spore germination and germ tube growth of U. necator, Phomopsis viticola, and Botrytis cinerea. The presence of both proteins displayed a synergistic effect. The expression of osmotin and thaumatin-like protein was induced in grapevine leaves and berries infected with U. necator and Phomopsis viticola. Thaumatin previously was thought to occur exclusively in berries. Immunoblot analyses revealed the accumulation of the two PR proteins in infected leaves and berries, supporting a role in vivo in increasing the resistance of grapevine to fungal attack.

Constitutive Coumarin Accumulation on Sunflower Leaf Surface Prevents Rust Germ Tube Growth and Appressorium Differentiation

Crop Science ◽  
2007 ◽  
Vol 47 (3) ◽  
pp. 1119-1124 ◽  
Author(s):  
E. Prats ◽  
M. J. Llamas ◽  
J. Jorrin ◽  
D. Rubiales
Keyword(s):  
Germ Tube ◽  
Leaf Surface ◽  
Tube Growth ◽  
Germ Tube Growth
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