Quantitative Differences in Nuclear Dna Content Between Armillaria Mellea and Armillaria Bulbosa

Armillaria mellea, a phytopathogenic fungus, is the only member of the Agaricales (Basidiomycetes) whose fertile vegetative phase in nature is thought to be diploid, rather than dikaryotic. To examine the vegetative ploidy of A. mellea, we used the DNA-binding antibiotic, mithramycin, for fluorometry of in situ nuclear DNA. The measurements of nuclear DNA content indicated that strains derived from single basidiospores of A. mellea were haploid and that strains derived from matings of isolates of single spores were diploid. These data confirm the results of earlier genetic experiments, which show haploidy and diploidy in unmated and mated strains, respectively. Nuclear DNA measurements in known haploid and diploid strains of Aspergillus nidulans confirmed the validity of our protocol.

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EVIDENCE FOR SIGNIFICANT DIFFERENCES IN NUCLEAR DNA CONTENT BETWEEN TWO GEOGRAPHICALLY ISOLATED CLONES OF ARMILLARIA MELLEA

American Journal of Botany ◽

10.1002/j.1537-2197.1985.tb08383.x ◽

1985 ◽

Vol 72 (8) ◽

pp. 1307-1310 ◽

Cited By ~ 4

Author(s):

Jerome J. Motta

Keyword(s):

Dna Content ◽

Nuclear Dna ◽

Nuclear Dna Content ◽

Armillaria Mellea ◽

Geographically Isolated

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Quantitative Differences in Nuclear DNA Content between Armillaria mellea and Armillaria bulbosa

Mycologia ◽

10.2307/3807438 ◽

1986 ◽

Vol 78 (6) ◽

pp. 963 ◽

Cited By ~ 9

Author(s):

Jerome J. Motta ◽

Diane Cope Peabody ◽

Robert B. Peabody

Keyword(s):

Dna Content ◽

Nuclear Dna ◽

Nuclear Dna Content ◽

Armillaria Mellea

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Nuclear DNA content of benomyl-induced segregants of diploid strains of the phytopathogenic fungus Armillaria mellea

Canadian Journal of Genetics and Cytology ◽

10.1139/g85-009 ◽

1985 ◽

Vol 27 (1) ◽

pp. 47-50 ◽

Cited By ~ 5

Author(s):

J. B. Anderson ◽

D. M. Petsche ◽

A. L. Franklin

Keyword(s):

Dna Content ◽

Nuclear Dna ◽

Nuclear Dna Content ◽

Marker Locus ◽

Phytopathogenic Fungus ◽

Armillaria Mellea ◽

Auxotrophic Marker ◽

Dna Contents ◽

Marker Loci ◽

Nuclear Dna Contents

The relative nuclear DNA contents of haploid, diploid, and benomyl-induced segregants of diploid strains of the phytopathogenic fungus Armillaria mellea were measured by mithramycin staining and fluorescence photometry. The diploid strains, originally recovered from sexually compatible matings of haploid strains, were heterozygous at mating-type and auxotrophic marker loci. The somatic segregants examined here were derived by treatment of the diploid strains with the fungicide benomyl in previous studies. As expected, the diploid strains had approximately twice as much nuclear DNA as the haploid strains. Most segregants had near-haploid DNA contents and no detectable heterozygosity at the marker loci; these strains were most likely true haploids. Other segregants with near-haploid DNA contents were heterozygous at a marker locus indicating that they were aneuploid. A minority of segregants had near-diploid DNA contents and may have been either aneuploid or diploid.Key words: basidiomycetes, mithramycin, parasexuality.

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