Biogeography and Genetic Diversity of Pearl Millet (Pennisetum glaucum) from Sahelian Africa

Aim: The present study was undertaken to analyze genetic diversity among pearl millet genotypes based on drought linked morpho-physiological and microsatellite markers. Study Design: In the present investigation, 96 pearl millet germplasm lines were screened against drought using different morphological and physiological traits along with SSR markers. Place and Duration of the Study: The present study was conducted at College of Agriculture, Gwalior, Rajmata Vijayaraje Scindia Krishi Vishwa Vidyalaya, Gwalior, M.P., India during July 2019 to December, 2020. Methodology: The study was conducted to record different morphological and physiological traits related to drought tolerance and susceptibility. Thirty five microsatellite markers were also used in laboratory to analyze the variability among pearl millet genotypes under study. Results: Pearl millet genotypes were grouped according to their morpho-physiological characteristics. Among 35 SSR markers, twenty-two were successfully amplified across all germplasm lines and seven SSR markers were found to be polymorphic and fifteen markers were monomorphic. All seven polymorphic SSR markers were used consequently for amplification of all the 96 germplasm lines. The range of PIC value was 0.0939 to 0.2980 with the average of 0.2274. The highest PIC value was recorded for the markers Xibmsp26 and Xibmsp29 (0.2980), followed by Xibmsp03 (0.2392), Xibmsp29 (0.2392), Xibmsp06 (0.2289) and Xibmsp07 (0.1948) while the lowest for the marker Xibmsp01 (0.0939). The range of major allele frequency value was 0.7604 to 0.9479 with the average of 0.8363. The range of genetic diversity value was 0.0987 to 0.3644 with the average of 0.2665. Conclusions: According to the morpho-physiological data a total of 22 pearl millet genotypes were found to be grouped distantly from rest of the genotypes. These genotypes had shown their drought tolerance bahaviour however, rests of the genotypes were found to be susceptible against drought.

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Genetic diversity in seed and restorer parents in relation to grain yield and its component traits in pearl millet [Pennisetum glaucum (L.) R. Br.]

Electronic Journal of Plant Breeding ◽

10.5958/0975-928x.2017.00071.0 ◽

2017 ◽

Vol 8 (2) ◽

pp. 465 ◽

Cited By ~ 1

Author(s):

Satbeer Singh ◽

S.K. Gupta ◽

S.K. Pahuja ◽

Devvart Yadav ◽

Anil Kumar ◽

...

Keyword(s):

Genetic Diversity ◽

Grain Yield ◽

Pearl Millet ◽

Pennisetum Glaucum ◽

Component Traits

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Molecular characterization of pearl millet [Pennisetum glaucum (L.) R. Br] inbreds using microsatellite markers

Journal of Applied and Natural Science ◽

10.31018/jans.v9i1.1196 ◽

2017 ◽

Vol 9 (1) ◽

pp. 357-363 ◽

Cited By ~ 1

Author(s):

Mamta Nehra ◽

Mukesh Kumar ◽

Dev Vart ◽

Jyoti Kaushik ◽

Rajesh Kumar Sharma

Keyword(s):

Genetic Diversity ◽

Linkage Group ◽

Pearl Millet ◽

Pennisetum Glaucum ◽

Polymorphic Information Content ◽

Minimum Variance ◽

Stay Green ◽

Ssr Loci ◽

Group A ◽

Clustering Pattern

Studies on genetic diversity in Pennisetum germplasm are the promising opportunities for the use of un-domesticated materials for improving pearl millet varieties. DNA based markers have now emerged as a potential genomic tool for estimation of genetic diversity among various cultivars and varietal identification. In present study, genetic diversity among 49 stay green inbreds of pearl millet was studied using simple sequence repeats (SSRs). Twenty nine polymorphic SSR primers, identified after initial screening of 70, were used to study diversity among these lines. A total of 108 alleles were amplified, collectively yielding unique SSR profiles for all the 49 inbreds. The average number of SSR alleles per locus was 3.72, with a range from 2 to 13. Polymorphic information content (PIC) values of various SSR loci across all the 49 inbreds ranged from 0.14 to 0.87 with an average of 0.51 per lo-cus. This indicated sufficient diversity among the 49 pearl millet inbreds and total 5 out of 29 polymorphic SSR loci, namely Xpsmp2070, Xpsmp2001, Xpsmp2008, Xpsmp2066, Xpsmp2072 revealed PIC values above 0.70, can be considered highly useful for differentiation of pearl millet inbred lines. The lowest PIC value (0.47) for linkage group 7 showed comparatively conserved nature of this linkage group A dendrogram obtained using WARD’s minimum variance method further delineates 49 inbreds into 8 major clusters, and the clustering pattern corroborated with their pedigree and characteristics traits.

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