Purification of Acidic Synthetic Peptides by High Performance Liquid Chromatography Using Ammonium Acetate Buffer

1988 ◽  
Vol 11 (8) ◽  
pp. 1651-1660 ◽  
Author(s):  
L. Lozzi ◽  
M. Rustici ◽  
A. Santucci ◽  
L. Bracci ◽  
S. Petreni ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Synthetic Peptides ◽  
High Performance ◽  
Ammonium Acetate ◽  
Acetate Buffer ◽  
Ammonium Acetate Buffer

scholarly journals Phenobarbital Analysis in Biological Matrix (Blood) by High Performance Liquid Chromatography (HPLC)

2013 ◽  
Vol 20 ◽  
pp. 31-40
Author(s):  
Ouakouak Hamza ◽  
Ben Mohamed Moktar ◽  
Ben Chohra Mostafa ◽  
Abdelhamid Zeghdaoui
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Mobile Phase ◽  
High Performance ◽  
Ammonium Acetate ◽  
Analytical Techniques ◽  
Hplc Method ◽  
Diode Array ◽  
Blood Components ◽  
Ammonium Acetate Buffer

In this work, we have tried to contribute to the analysis of phenobarbital in the blood. To do this, we used different analytical techniques such as liquid chromatography (HPLC). The results obtained in the course of our work, we can conclude that: The HPLC method was separate phenobarbital endogenous blood components, and optimizing the conditions of chromatographic separation as the composition of the mobile phase consisting of 20% acetonitrile + 20% methanol + 60% ammonium acetate buffer. The extraction with diethyl ether to pH = 4; The chromatographic column, microbondapack ZORBAX C18. A system of diode-array UV detection at 254 nm.

High Performance Liquid Chromatography Using Ultrasonic Extraction for Benzoic Acid Analysis in Curry Paste Samples

2019 ◽  
Vol 886 ◽  
pp. 40-45
Author(s):  
Nararat Thongsrinoon ◽  
Netnapha Phiwdee ◽  
Yanada Duangsa ◽  
Khaengkhae Muensub ◽  
Vichayaporn Duang-Iad
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Benzoic Acid ◽  
Mobile Phase ◽  
High Performance ◽  
Ammonium Acetate ◽  
Ultrasonic Extraction ◽  
Optimum Conditions ◽  
Ammonium Acetate Buffer ◽  
Uv Visible

Benzoic acid analysis in curry paste samples were carried out by using high performance liquid chromatography using ultrasonic extraction. Methanol-0.05 M ammonium acetate buffer pH 4.40 in the ratio of 55:45 (%v/v) at a flow rate of 1.00 mL/min was used as the mobile phase and benzoic acid detection was performed at 226 nm using an UV-Visible detector. Under the optimum conditions, linearity of spiked samples ranged from 50 to 3,000 mg/kg. Matrix matched calibrations had determined that benzoic acid contents in southern sour, red, and green curry paste samples were 67.59, 78.62 and 72.33 mg/kg, respectively. Recoveries were obtained from 89.34 to 101.70%, 83.37 to 130.30% and 92.75 to 113.56% with R.S.D. ranged from 2.71 to 6.53%, 4.02 to 11.58% and 5.81 to 6.35%, for southern sour, red, and green curry paste samples, respectively.

Analysis of Bifenthrin and its Enantiomer Using High Performance Liquid Chromatography

2014 ◽  
Vol 675-677 ◽  
pp. 275-279 ◽  
Author(s):  
Su Su Fan ◽  
Jian Shi ◽  
Ling Zhou ◽  
Yu Wen Hang
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Mobile Phase ◽  
High Performance ◽  
Chiral Stationary Phase ◽  
Ammonium Acetate ◽  
Hplc Method ◽  
Column Temperature ◽  
Polysaccharide Derivatives ◽  
Separation Results

Using the high performance liquid chromatography (HPLC) method, bifenthrin isomers can be split at a polysaccharide derivatives chiral stationary phase column, and two well distinguished peaks of bifenthrin isomers are obtained. The effects of mobile phase ratios, temperatures, and detection wavelengths on the separation results are discussed. The optimal chromatographic conditions are as follows: the mobile phase ratio is methanol: ammonium acetate salts = 80:20, the column temperature is 35°C, and the wavelength is set as 220 nm. Under the optimal conditions, the resolution of bifenthrin enantiomer can be as large as 3.0.

Ovarian and circulating levels of oxytocin and arginine vasopressin during the estrous cycle in the rat

1992 ◽  
Vol 126 (6) ◽  
pp. 530-534 ◽  
Author(s):  
Mei-Ling Ho ◽  
Jau-Nan Lee
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Estrous Cycle ◽  
Arginine Vasopressin ◽  
Synthetic Peptides ◽  
High Performance ◽  
The Other ◽  
Significant Difference ◽  
Long Evans ◽  
Circulating Levels

Ovarian extracts of Long-Evans rats separated using high performance liquid chromatography (HPLC) were measured by radioimmunoassays (RIAs) for the presence of oxytocin and arginine vasopressin (AVP). The results showed that the ovary contains both, and that they are indistinguishable from the respective standard synthetic peptides. During the estrous cycle, the ovarian content of oxytocin was 10-fold higher (p<0.01) in estrus than in the other phases, while AVP was 16- and 25-fold higher (p<0.01) in metestrus than in the other phases. In contrast, the plasma levels of oxytocin showed no significant difference among the various phases of the estrous cycle. However, the plasma level of AVP level was significantly higher (p<0.01) in diestrus than in other phases. The present study thus strongly supports the hypothesis that both oxytocin and AVP can be produced by the ovary itself in the rat. The possible roles of oxytocin and AVP in the reproductive cycle are discussed.

Xylitol production by Petromyces albertensis grown on medium containing D-xylose

1991 ◽  
Vol 37 (1) ◽  
pp. 14-18 ◽  
Author(s):  
Jagroop S. Dahiya
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Key Words ◽  
Yeast Extract ◽  
High Performance ◽  
Ammonium Acetate ◽  
Xylitol Production ◽  
Maximum Production ◽  
Initial Ph ◽  
Fermentative Products

Petromyces albertensis produced xylitol and D-xylulose when cultivated on a medium containing D-xylose. These fermentative products were identified by high-performance liquid chromatography. A large amount of xylitol was obtained from a D-xylose medium containing ammonium acetate and yeast extract at an initial pH of 7.0. Maximum production of xylitol and of the enzymes concerned with its production was observed after 10 days of cultivation. A D-xylose (100 g/L) medium supplemented with 1% (v/v) methanol gave the highest yields of xylitol (39.8 g/L) and D-xylulose (2.8 g/L). Key words: Petromyces albertensis, D-xylulose, xylitol.

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