An ESP Production Optimization Algorithm Applied to Unconventional Wells

The objectives and Scope is to evaluate the feasibility of a Production Maximization algorithm for ESPs on unconventional wells using projected operating conditions instead of current ones, which authors expect will be crucial in adjusting the well deliverability to optimum frequencies on the rapidly changing conditions of tight oil wells. Actual production data for an unconventional well was used, covering from the start of Natural Flow production up to 120 days afterwards. Simulating what the production would be if a VFD running on IMP Optimization algorithms had been installed, new values for well flowing pressures were calculated, daily production scenarios were evaluated, and recommended operating frequencies were plotted. Result, observations, and conclusions: A. Using the Intelligent Maximum Production (IMP) algorithm allows maximum production from tight oil wells during the initial high production stage, and the prevention of gas-locking at later stages when gas production increases. B. The adjustment of frequency at later stages for GOR wells is key to maintaining maximum production while controlling free gas at the intake when compared against controlling the surface choke. Novel/additive information: The use of Electrical Submersible Pumps for the production of unconventional wells paired with the use of a VFD and properly designed control algorithms allows faster recovery of investment by pumping maximum allowable daily rates while constraining detrimental conditions such as free gas at the intake.

Maximum production point tracking method for a solar-boosted biogas energy generation system

Low biogas yield in cold climates has brought great challenges in terms of the flexibility and resilience of biogas energy systems. This paper proposes a maximum production point tracking method for a solar-boosted biogas generation system to enhance the biogas production rate in extreme climates. In the proposed method, a multi-dimensional R–C thermal circuit model is formulated to analyze the digesting thermodynamic effect of the anaerobic digester with solar energy injection, while a hydrodynamic model is formulated to express the fluid dynamic interaction between the hot-water circulation flow and solar energy injection. This comprehensive dynamic model can provide an essential basis for controlling the solar energy for digester heating to optimize anaerobic fermentation and biogas production efficiency in extreme climates. A model predictive control method is developed to accurately track the maximum biogas production rate in varying ambient climate conditions. Comparative results demonstrate that the proposed methodology can effectively control the fermentation temperature and biogas yield in extreme climates, and confirm its capability to enhance the flexibility and resilience of the solar-boosted biogas generation system.

Analisa Pendapatan Usahatani Tanaman Pacar Air di Desa Dukuh Klopo Kecamatan Peterongan Kabupaten Jombang

Agriculture is a significant sector of the Indonesian economy. Farmers strive for economically advantageous outcomes in the processing of farming, where the costs paid can result in maximum production. This increases demand for flowers, one of which is water henna. Farmers must enhance production to fulfill the demand for water henna flowers. The purpose of this study is to determine the viability of water henna flower growing in Dukuh Klopo Village, Peterongan District, Jombang Regency. The analytical method employed in this research is a combination of descriptive and quantitative analysis, which will be used to determine the income generated by water henna farming. According to the feasibility analysis results, the B/C – Ratio for water henna plant cultivation in Dukuh Klopo Village, Peterongan District, Jombang Regency is 1.57. This means that for every Rp. 1 spent, the henna plant farming industry in Dukuh Klopo Village, Peterongan District, Jombang Regency generates Rp. 1.57 rupiah in revenue. Thus, the B/C Ratio approach enables the development of water henna flower farming in Dukuh Klopo Village, Peterongan District, Jombang Regency.

​Optimization of Cultural Conditions for Maximum Production of Outer Membrane Vesicles from Salmonella Typhimurium

Background: The non-typhoidal Salmonella causes gastroenteritis in humans that makes its way to the food chain mainly through the animal products. The multiple drug resistance imposes one of the major hurdle in the treatment of the disease. The vaccination appears to be the most important method for prevention of the disease. Unfortunately, there is no liscenced vaccine available against non-typhoidal Salmonellae. The use of outer membrane vesicles (OMVs) of Salmonella as a vaccine candidate has attained significant centre-stage in the recent years given to its protective immunogenicity. However, the large scale production of OMVs is difficult owing to low yield per liter of culture. Methods: In the present study, we have optimized the culture conditions viz. pH, phase of growth and presence of oxidative stress for maximum production of OMVs from Salmonella Typhimurium. The OMVs were characterized based on yield based on protein concentration, lipopolysaccharide concentration and zeta size. Result: In the present study, it was found that incubation of Salmonella Typhimurium up to peak of the growth phase at pH 7 in presence of oxidative stress was found to be the most suitable condition for maximum production of OMVs.

Production of Hydroxyl Radicals From Oxygenation of Simulated AMD In Presence of Extracellular Polymers Substances

While the reaction mechanisms Fe(II) abiotic oxidation produce ·OH by CaCO3-induced in AMD are well-documented, little is known about the influence of extracellular polymeric substances (EPS) secreted by microorganisms on Fe(II) oxidation in AMD. Given the recent finding, this study experimently measured the cumulative concentrations of ·OH produced from oxygenation of simulated AMD in the presence of EPS. Results of this study show that the cumulative ·OH increased from 56.75 to 158.70 μM within 24 h at pH 3 with the increase in EPS concentration from 0 to 12 mg/L. An appropriate pH (about 6) and EPS (6 mg/L) concentration were required for the moderate rate of Fe(II) oxidation, corresponding to the maximum production of ·OH. The presence of EPS enhanced the ·OH production from Fe(II) oxidation in simulated AMD under acid conditions. In the presence of EPS, ·OH production is attributed mainly the complexation of Fe(II) with EPS, of which is rich of carboxyl and hydroxyl groups. Besides, the yield of ·OH increased remarkably with the addition of Fe3+. It is most likely that EPS can contribute to reduce Fe(Ⅲ) to Fe(II), which is beneficial to the production of ·OH. The findings reveal from this study supplement the fundamental of ·OH production from Fe(II) oxidation by microorganisms in natural AMD.

Bioprocess Optimization of Glutathione Production by Saccharomyces Boulardii: Biochemical Characterization of Glutathione Peroxidase

The well-known probiotic GRAS Saccharomyces boulardii (CNCM I-745) was used for the first time to produce glutathione (GSH). The culture conditions affecting GSH biosynthesis were screened using a Plackett-Burman design (PBD). Analyzing the regression coefficients for 12 tested variables; 6 of them, including yeast extract, glucose, peptone and cysteine; temperature and agitation rate had a positive significant effect on GSH production with a maximum production of 192 mg/L. The impact of addition time of cysteine was investigated in 19 experiments during the growth time course (0-36 h), the best addition time was 8h post-inoculation producing 235 mg/L of GSH. The most significant variables were further explored at 5-levels using Central Composite Rotatable Design (CCRD), giving a maximum production of GSH (552 mg/L). Using baffled flasks, the GSH was increased to 730 mg/L, i.e 1.32-folds increment than obtained using CCRD. The two rate-limiting genes of GSH biosynthesis “γ-glutamyl cysteine synthetase (gsh1) and GSH-synthetase (gsh2) were amplified and sequenced to validate the GSH biosynthetic potency of S. boulardii. The sequences of genes showed 99% similarity with gsh1 and gsh2 genes of S. cerevisiae. Glutathione peroxidase was purified and characterized from S. boulardii with molecular mass and subunit structure of 80 kDa and 35 kDa as revealed from native and SDS-PAGE, ensuring its homodimeric identity. The activity of GPx was reduced by 2.5-folds upon demetallization confirming its metalloproteinic identity. The enzyme was strongly inhibited by hydroxylamine and DTNB, ensuring the implication of surface lysine and cysteine residues on the enzyme active site domains.

Importance of spindle speed in ring frame

In textile industries, spinning sector plays a vital role because this sector produces the main raw material for the textile industries which is known as yarn. So for getting good qualities garments, it is needed to ensure the good qualities yarn. There are many things which are re-sponsible for producing good qualities yarn. Among these spindle speed is an important thing. One must have to change the spindle speed, when he changes the count. As every count has a particular spindle speed in which the production shows maximum production efficiency. Here we used five most commonly used yarn counts in the maximum factories. The counts are 15 Ne, 20 Ne, 25 Ne, 30 Ne and 40 Ne. We analyzed these counts with different speed and found that every count showed maximum efficiency at a particular spindle speed. The out-come of this paper is applicable in all types of ring frame machine , especially for producing single carded and combed yarn.

Keratinolytic protease from Pseudomonas aeruginosa for leather skin processing

Background The leather industry generates huge volume of waste each year. Keratin is the principal constituents of this waste that is resistant to degradation. Some bacteria have the ability to degrade keratin through synthesis of a protease called keratinase that can be used as sources of animal feed and industrial production of biodiesel, biofertilizer, and bioplastic. Majority of the studies focused on keratin degradation using gram-positive bacteria. Not much of studies are currently available on production of keratinase from gram-negative bacteria and selection of best parameters for the maximum production of enzyme. The aim of this study was to isolate and characterize both groups of bacteria from soil for keratinase and optimize the production parameters. Results A total of 50 isolates were used for initial screening of enzyme production in skim milk, casein, and feather meal agar. Out of 50, five isolates showed significantly higher enzyme production in preliminary screening assays. Morphological and biochemical characterization revealed 60% of the isolates as gram-negative bacteria including two highest enzyme-producing isolates. The isolates were identified as Pseudomonas aeruginosa through sequencing of 16S rRNA gene. Maximum production of enzyme from P. aeruginosa YK17 was achieved with 2% chicken feather, beef extract, and ammonium nitrate as organic and inorganic nitrogen sources and glucose as a carbon source. Further analysis revealed that 3% inoculum, 40 °C growth temperature and 72-h incubation, resulted in maximum production of keratinase. Conclusion The overall results showed significant higher production of enzyme by the P. aeruginosa YK17 that can be used for the degradation of recalcitrant keratin waste and chemical dehairing in leather industries, thereby preventing environmental pollution.

Carbon and Nitrogen Sources Effect on Pectinase Synthesis by Aspergillus niger Under Submerged Fermentation

Pectinases are the commercial enzymes that are abundantly employed in various industries like fruit juice industries for clarification, wine indutsry and paper industry for bleaching up pulp. The present work was done on culture conditions optimization for production of pectinases under submerged fermentation using wheat bran as a substrate. Fungal strains were isolated from vegetable waste dump yard soils of Warangal district of Telangana state and screened for their activity on pectin agar medium. Among 30 isolates, two fungal strains showed good activity and identified them as A. niger and A. flavus. The effects of the different carbon and nitrogen sources on pectinases viz. exo-PG, endo-PG, endo-PL and PME by A. niger with 1% wheat bran was carried out in submerged fermentation. These studies revealed that carbon and nitrogen sources have shown considerable influence on enzyme production. Among all the carbon sources tried, sucrose at 1% was shown to be efficient carbon source for all four types of pectinases under investigation. For endo-PG, endo-PL and PME maximum enzyme production were recorded on 8th day of incubation period but for exo-PG enhanced production was observed on 12th day. A. niger could not produce PME on 12th day from 2.50% to subsequent concentrations. Among nine different nitrogen sources were screened, maximum pectinase production was recorded in sodium nitrate at 0.2 % for A. niger. Endo-PG, endo-PL and PME maximum production were recorded on 8th day of incubation and for exo-PG maximum production was observed on 12th day. No PME production was observed in A. niger on 12th day.