Thin-Layer Chromatography of Metal Ions in Formic Acid Medium on Impregnated and Unimpregnated Silica Gel G: Semiquantitative Determination of Fe3+, Cd2+, Th4+, Al3+, Zn2+, UO2+2, VO2+, Ce4+, and Ni2+

An isolate of Fusarium sporotrichioides Sherbakoff obtained from hay from Kindersley, Saskatchewan, and producing metabolites toxic to insect larvae, rats, and mice was cultured on sterile rye grain and extracted with ethyl acetate. The most toxic fraction derived from column chromatography on silica gel was purified by thin-layer chromatography on silica gel and elution of the main band. This compound was identified as T-2 toxin by comparison with the pure chemical by thin-layer chromatography and, after crystallization, by determination of melting point, infrared spectrum, and nuclear magnetic resonance spectrum. This work demonstrates that strains of F. sporotrichioides capable of producing T-2 toxin may be present on hay in western Canada.

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Determination of Aflatoxins in Animal Tissues

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/64.4.964 ◽

1981 ◽

Vol 64 (4) ◽

pp. 964-968

Author(s):

Robert D Stubblefield ◽

Odette L Shotwell

Keyword(s):

Citric Acid ◽

Thin Layer ◽

Thin Layer Chromatography ◽

Silica Gel ◽

Determination Limit ◽

Animal Tissues ◽

Freeze Dried ◽

Human Livers ◽

Layer Chromatography

Abstract A method for the determination of aflatoxins in animal tissues has been developed, and applied successfully to beef, swine, chicken, and human livers, and to beef kidney, heart, spleen, muscle, and blood. Blended tissue is denatured with citric acid and extracted with dichloromethane on a wrist-action shaker. After filtration, the extract is partially purified on a silica gel column, and aflatoxins B1 and M1 are determined by 2-dimensional thin layer chromatography and densitometry. Recoveries of Bi and Mi added to meat tissues and blood were approximately 90 and 80%, respectively. The method gave results for a contaminated freeze-dried liver comparable to analyses by 3 other published meat tissue methods. The method is rapid and has a determination limit ≤0.1 ng/g. In addition, the method uses less toxic and smaller quantities of solvents and chemicals.

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Improved Method for the Thin Layer Chromatographic Determination of Patulin in Apple Juice

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/56.4.813 ◽

1973 ◽

Vol 56 (4) ◽

pp. 813-816 ◽

Cited By ~ 7

Author(s):

Peter M Scott ◽

Barry P C Kennedy

Keyword(s):

Thin Layer ◽

Ethyl Acetate ◽

Thin Layer Chromatography ◽

Silica Gel ◽

Apple Juice ◽

Chromatographic Determination ◽

Improved Method ◽

Layer Chromatography ◽

Hydrochloride Solution

Abstract Apple juice from a freshly opened container is extracted 3 times with ethyl acetate. The extract is dried, concentrated, diluted with benzene, and added to a silica gel column. Patulin is eluted by benzene-ethyl acetate (75+25) and detected by thin layer chromatography, using a 3-methyl-2-benzothiazolinone hydrazone hydrochloride solution as the spray reagent. Satisfactory recoveries were obtained for patulin added to apple juice at levels of 25–400 μg/L.

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