Rhodococcus in foal, from diagnosis to treatment: Case report

The present work reports a case of a foal affected by Rhodococcus equi, an optional intracellular gram-positive pleomorphic cocobacillus, which, when eliminated with the feces of infected animals, in the soil becomes a potential pathogen, capable of causing mainly piogranulomatous bronchopneumonia. The animal, a 23-day-old Brazilian equestrian foal, was sent to the veterinary hospital with hyperthermia, increased heart and respiratory rates, crackle in tracheal auscultation, loss of appetite and prostration. After hematological, ultrasound, radiographic examinations, and collection of tracheal lavages for culture and PCR, the definitive diagnosis of rhodococcosis was obtained, which is a bacterial disease that mainly affects foals, has a worldwide distribution and has a great economic impact on equine culture. Based on the results, treatment with azithromycin, rifampin, flunixin meglumine, sucralfate, ranitidine hydrochloride and amoxicillin with clavulanate was instituted, associated with nebulization with acetylcysteine diluted to 0.9% sodium hydrochloride solution. After a few weeks, there was no evolution in the results of the ultrasound and radiographic exams, however the hematological exams were within the reference levels, there was a significant clinical improvement, and the animal was discharged. It is noteworthy that early diagnosis was essential for the effectiveness of treatment and remission of clinical signs. Keywords: rhodococcosis; foals; rhodococcus equi.

Assessment the Genotoxic Potential of Fluoxetine and Amitriptyline at Maximum Therapeutic Doses for Four-Week Treatment in Experimental Male Rats

At any moment, the continuous usage of medications can accompanied by DNA damage and the accumulation of such damages can cause serious consequences. Antidepressants are long-term used drugs and the incidence of their genotoxic impacts cannot be excluded. Therefore, this work was designed to investigate the possible genotoxic effects of the commonly used antidepressants (fluoxetine and amitriptyline) in adult male rats. Detection of DNA damage in individual cells was assessed by comet and micronucleus assays in three different cell populations i.e. liver, testis and bone marrow tissues of 24 swiss albino adult male rats. The animals were randomly allocated into three groups of 8 rats each: Group I - rats orally-administered distilled water via gavage tube for four weeks as a negative control. Group II - rats orally-treated with fluoxetine hydrochloride solution (7.2mg/kg/day) via gavage tube for four weeks. Group III - rats orally-treated with amitriptyline hydrochloride solution (27mg/kg/day) via gavage tube for four weeks. The results showed that both drugs (Group II and Group III) induced the same extent of DNA damage, as evidenced by a significantly higher DNA fragmentation in liver and testis tissues with increased frequencies of micronuclei formation in bone marrow tissues as compared with the negative control (Group I). These findings indicates that both Fluoxetine and Amitriptyline have genotoxic potentials and can induce the same extent of cytogenetic damage in rats. Special precautions and medical supervision should be taken in consideration with their uses.

PECULIARITIES OF TREATMENT OF PATIENTS WITH ONYCHOMYCOSIS WITH CONTRAINDICATIONS TO SYSTEMIC ANTIMYCOTICS

The aim: To increase the effectiveness of treatment of patients with onychomycosis with contraindications to administration of systemic antifungal therapy. Materials and methods: The study involved 63 patients with onychomycosis of the toes aged 18 to 72 years under supervision at the clinic of the Institute of Dermatology and Venereology of the National Academy of Medical Sciences of Ukraine, who were divided into groups: Group I (30 patients) received naftifine hydrochloride solution and cream; Group II (33 patients) additionally used nail kit mycosan and cytoflavin 2 tablets 2 times a day for 1-1.5 months.The study implied microscopic and molecular genetic examination of nail plates before treatment and starting from the 4th week every 2 weeks. The study included patients with onychomycosis with positive results of both microscopic and PCR studies. Mycological recovery was noted in case of coincidence of results of these studies. Results: Mycological recovery in patients of Group II began in the sixth week in 6.3% and occurred in 100% of cases at the 24th week of treatment, depending on the severity and clinical form. The overall effectiveness of therapy at the 48th week reached 56.7% in Group I and 72.7% in Group II. Conclusions: The developed method of treatment, which includes the use of naftifine hydrochloride solution and cream, nail kit micozan and cytoflavin, allows to achieve clinical and mycological recovery in 72.7% of patients and is a priority in the treatment of patients with onychomycosis with comorbid conditions.

Comprehensive treatment of dogs with chronic generalized periodontitis in remission

It is known that periodontal diseases are a significant part of dental diseases in dogs. Chronic generalized periodontitis deserves the most attention. The disease develops slowly, beginning without visible clinical signs, but later, a combination of certain favorable etiological factors can lead to the loss of the animal's teeth, indigestion, metabolism with serious consequences for the dog. Therefore, the search of the features of the disease and the development of tactics for effective treatment of periodontally ill animals is relevant for modern veterinary dentistry. The aim of the work was to study the features of the course of chronic generalized periodontitis in remission in dogs and to develop tactics for rational treatment of periodontally ill animals. For the study, two groups of animals with chronic generalized periodontitis were formed in remission with 5 dogs in each (control and experimental), selected on the principle of analogues in terms of age and nature of the pathological process. Searches have shown that in chronic generalized periodontitis in remission in dogs there is an increase in the number of pathogenic microflora in the biotopes of the oral cavity, increasing the activity of acid phosphatase, increasing the protein concentration by reducing the content of lysozyme in the oral fluid. It was found that the dogs of the experimental group, after removal of tartar and rehabilitation of the oral cavity, were treated by using to the gums of ceftriaxone, diluted in 1% lidocaine hydrochloride solution in combination with 30 % dimexid solution 1 time per day for 7 days, anfluron injecteed intramuscularly at the rate of 1 ml 1 time per day for 7 days and caforsen – 0.1 ml/kg per day for 4 weeks. The treatment contributed to the recovery of dogs for 10 days, which is 8 days faster than in the control group.

Study of the Stability of a Viscous Solution of Naftifin Hydrochloride with a Combination of Polyethylene Glycols for External Use

Introduction. An innovative antifungal viscous solution based on naftifine hydrochloride with a combination of polyethylene glycols (PEG) was developed in the laboratory of Sechenov University. The developed preparation intended for external use. The active ingredient – naftifine hydrochloride has a wide spectrum of action against fungi cause onychomycosis. The polyethylene glycols are included in the developed dosage form of provide the necessary viscosity of the solution (for accurate application and retention in the field of application). The paper presents the results of a study of the stability of a viscous solution of naftifine hydrochloride with a combination of PEG for external use. Over the entire shelf life, the drug must retain the full its chemical, physical, biopharmaceutical and pharmacological properties.Aim. Determination of stability and expiration date of the shelf life of the developed solution of naftifine hydrochloride for external use, intended for the treatment of mycosis of the nail.Materials and methods. Naftifine hydrochloride solution, «Millipor» filter, UV spectrophotometry, potentiometry pH, capillary viscometry.Results and discussion. In the course of the study, the shelf life of the developed alcohol solution of naftifin hydrochloride with a combination of PEG was experimentally determined. The stability of the dosage form was determined by accelerated aging at a temperature of 40 ± 2 °C, in vivo at a temperature of no higher than 25 °C; and in a refrigerator at a temperature of 8 ± 2 °C. Assessment of the stability of the alcohol solution of naftifine hydrochloride was carried out according to the following indicators: the volume of the contents of the bottle, appearance, pH, quantitative content of the active substance, viscosity.Conclusion. Based on the studies, it is recommended to store the naftifine hydrochloride solution at room temperature not higher than 25 °C, in a dark place. It is also allowed to store the solution of naftifine hydrochloride in a refrigerator at a temperature of 8 ± 2 °C.