Considerable interest has been aroused by the report of Gillman, Gilbert, Gillman, & Spence (1948) that the vital dye trypan blue when injected into the pregnant rat brings about the appearance of various abnormalities in the offspring. The mammal embryo is notoriously difficult to attack experimentally, and trypan blue therefore seems to provide a valuable tool which, moreover, might throw light on the mechanisms of the important effects which are known in some cases to be produced on the foetus by pathological states in the mother. Further studies on the effects of injected trypan blue on mammalian embryos have therefore been made by Hamburgh (1952, 1954), Waddington & Carter (1952, 1953), and Murakami and his collaborators (1952, 1954), all of whom worked on the mouse, and by Harm (1954) who studied the rabbit. All these authors agree, on the whole, in the type of results which they have obtained, although there are some minor differences between their reports which will be considered later.
Comparison of wash-out properties after use of the vital dye trypan blue in the form of an ophthalmic dye and bound in a sodium hyaluronate by Raman spectroscopy
