Effect of a Tea Polyphenol on Different Levels of Exposure of Nicotine and Tobacco Extract on Streptococcus mutans Biofilm Formation

Objective: The purpose of this study was to compare the effects of different levels of nicotine and tobacco extract exposure on Streptococcus mutans biofilm formation and the inhibitory effect of the polyphenol epigallocatechin-3 gallate (EGCG) found in green tea. This study addressed the results of biofilm assays with EGCG and varying relative concentrations of nicotine and tobacco extract consistent with primary, secondary and tertiary levels of smoking exposure. Primary smoking exposure to nicotine has been demonstrated to significantly increase biofilm formation, while EGCG has been demonstrated to reduce S. mutans biofilm formation.Methods:S. mutans was treated with varying levels of nicotine or cigarette smoke condensate (CSC) concentrations (0–32 mg/ml and 0–2 mg/ml, respectively) in Tryptic Soy broth supplemented with 1% sucrose for different lengths of time simulating primary, secondary and tertiary smoking exposure with and without 0.25 mg/ml EGCG. The amount of total growth and biofilm formed was determined using a spectrophotometric crystal violet dye staining assay.Results: For both nicotine and CSC, primary exposure displayed overall significantly less growth compared to secondary exposure. For nicotine, secondary exposure demonstrated significantly greater growth than tertiary exposure levels. Overall, significantly greater total bacterial growth and biofilm formation in the presence of nicotine and CSC was observed in the absence of EGCG than in the presence of EGCG. However, biofilm growth was not significantly different among different concentrations of CSC.Conclusion: The results of this study help illustrate that nicotine-induced S. mutans biofilm formation is reduced by the presence of EGCG. This provides further evidence of the potential beneficial properties of polyphenols.

Involvement of Follicular Patency in the Ovarian Developmental Block in Virus-infected, MdSGHV, House Flies, Musca domestica (Diptera: Muscidae)

The Musca domestica salivary gland hypertrophy virus (MdSGHV) is known to have marked effects on the female Musca domestica L. (or common house fly) reproductive system, particularly regarding the size and functionality of the ovaries. Examination of the terminal ovarian follicles can help determine if and how MdSGHV mechanistically causes the block in ovarian development. In this study, terminal ovarian follicle lengths were measured and monitored for patency using Trypan blue dye staining. We examined the effect of MdSGHV infection on female house fly ovarian follicles and attempted to rescue the diminished ovarian follicles in MdSGHV-infected house flies through the application of a hormonal treatment (i.e., methoprene). Comparison of patency in control saline-injected females, virus-injected females with no methoprene application, and virus-injected females with topical methoprene application revealed that none of the virus-infected flies showed an increase in terminal follicular length beyond stage 3 follicles (staging according to Adams 1974). Additionally, none showed evidence of patency. In control, saline-injected females, we found the threshold length of the terminal follicles for the onset of patency to be 600 µm. When examined at 48, 72, and 96 h post-eclosion, average follicle length for infected females seldom reached 250 µm and they also failed to display patency. Thus, the virus is somehow involved in shutting down the mechanism involved in follicular patency. The lack of patency in infected follicles may also be one of the determining factors preventing vertical transmission of the pathogen.

Spatial heterogeneity of soil properties and solute transport characteristics and their correlations in degraded wetland soils

Soil properties have a significant influence on solutes redistribution in the soil vadose zones. The aim of this study was to assess the relevance of soil properties for solute transport characteristics in degraded wetland soils using 72 undisturbed soil columns from two experimental fields located in Robinia pseudoacacia (CH) and Tamarix chinensis (CL) communities. Combining soil column tracer experiments, all experiments were conducted under the same initial and boundary conditions using Brilliant Blue FCF as a conservative tracer. Solute transport characteristics were described by four measures of dye solution steady infiltration rate of effluents, dye solution concentration of effluents, soil column dye staining patterns, and cumulative dye solution leaching. Numerical modeling by the dual-permeability model in HYDRUS-1D was used to simulate the proportion of cumulative dye solution leaching from soil macropore flow. This study showed that basic soil properties exhibited a significant difference at CH site and at CL site. Dye solution steady infiltration rate of effluents at CH site decreased with soil depth, but increased at first and then decreased with soil depth at CL site. Dye solution concentration of effluents both at CH site and at CL site decreased nonlinearly with soil depth. Soil column dye staining patterns were significantly different among different soil locations, indicating the largest dark blue staining domains from soil depth of 0-10 cm at CH site and 20-40 cm at CL site. The proportion of cumulative dye solution leaching from soil macropore flow was from 37.6 to 61.1% at CH site, whereas from 0 to 99.9% at CL site. Basic soil properties played inconsistent roles in solute transport characteristics. The understanding of soil properties and its correlation with solute transport characteristics is the first step for degraded wetland restoration and development. Some alternative solutions of wetland restoration are proposed for managers.

A sono-anatomical and cadaveric study of ultrasound-guided retrolaminar block

Aim: Retrolaminar block (RB) is known to confer chest wall analgesia but, its mechanism has not been established. Our primary objective was to determine if the spread of injectate following RB extends to the paravertebral space (PVS). Second-ary objectives were to determine the predefined anatomical areas and nervous tissues contacted by injectate; the effect of volume on spread; and the extent to which experts can predict PVS spread based on examination of US videos of the injection. Material and methods: US-guided RB was performed on cadavers using a single injection technique of 10, 20 or 30 ml dye. Anatomical dissection was performed to identify the extent of spread of injectate to the retrolaminar, intercostal and PVS. Ultrasound recordings of the injection were independently evaluated by experts in US-guided regional anaesthesia. Results: Spread of injectate to the ipsilateral PVS was identified in 6/10 dissected regions (0/1, 1/3 and 5/6 when injectate volumes of 10, 20 and 30ml were administered respectively). The extent of cephalad-caudad spread within the PVS varied from 1 to 3 levels. Expert interpretation of ultrasound images regarding spread to the PVS demonstrated poor correlation with dye staining observed on dissection. Conclusions: Injectate spread following RB demonstrated substantial variability. Inconsistent spread to the ipsilateral PVS may account for clinically occurring incomplete blocks. The likelihood of spread to the ipsilateral PVS was greater when a larger volume was injected. Expert evaluation of the dynamic ultrasound images obtained at injection can-not reliably predict spread to the PVS.

Preoperative imaging optimized for epiretinal membrane surgery

Background To compare imaging modalities for visualizing primary epiretinal membrane (ERM) with each other and with intraoperative digital images (IDI) after blue staining. Methods The records of consecutive patients operated for primary ERM over a 12-month period were retrospectively reviewed. Preoperative imaging included color fundus photography (CFP), En Face spectral-domain optical coherence tomography (OCT), 45° infrared- (IR) and blue-reflectance (BR) scanning laser ophthalmoscopy. All images were qualitatively analyzed and scored from 0–4 according to the ability to visualize ERM details (0 = no visible ERM or vessel contraction, 1 = vessel contraction, 2 = retinal folds, 3 = ERM limits, 4 = elevated ERM edge). The preoperative ERM morphology was then compared to that seen on the IDI acquired after 1-min blue dye staining when available. Results Seventy eyes were included. The highest score for ERM visualization was obtained on BR and En Face OCT. A score of 3 or 4 was obtained in 68.5%, 62.1%, 17.9% and 13.6% of cases on En Face OCT, BR, CFP and IR images, respectively. IDI were available for 20 eyes, and showed a similar ERM morphology compared to preoperative images in most cases: a negative staining pattern corresponded to a plaque on En face OCT in 91% of eyes. However, IDI failed to show the ERM edges in 37.5% of cases. Conclusion ERM morphology was better visualized preoperatively by BR and En Face OCT, in a similar way to the IDI after staining. Future intraoperative visualization systems could integrate both imaging modalities overlaid with the IDI for guiding ERM removal instead of staining.

Characterizing Preferential Flow Paths in Texturally Similar Soils under Different Land Uses by Combining Drainage and Dye-Staining Methods

Preferential flow paths have been widely characterized by many visualization methods. However, the differences in preferential flow paths under various land uses and their relationships to hydraulic properties remain uncertain. The objectives of this study are to (1) characterize preferential flow paths under various land uses (forest and orchard) by combining drainage and dye-staining methods and to (2) build a connection between preferential flow paths and hydraulic-related parameters and extract the proportion of preferential flow paths from the compounding effects of matrix flow and preferential flow. The dye-staining experiments were conducted in five sandy soils and one sandy clay loam in situ, including four soils from forest and two soils from orchards. A total of 47 soil cores, 4 cm in height and 9 cm in diameter, were collected in each layer of the dye-stained soils for drainage experiments in the laboratory. Dye coverage and hydraulically equivalent macropore parameters (macroporosity, pore size distribution, and number of macropores) and their relationships were analyzed. The results show that the volume of preferential flow is partly affected by the total macropore volume. The effect of macropores on preferential flow varies by macropore size distribution. Dye coverage exhibited a significant (P < 0.01) correlation with macroporosity (correlation coefficient 0.83). Based on the value of macroporosity or steady effluent rates, the part of the dye coverage that was due to preferential flow on the surface dye-stained soil (resulting from both matrix and preferential flow) could be identified in this study. Compared with orchards, forestland has more preferential flow paths in both surface soil and subsoil. Further studies are needed to quantify the 3-D preferential flow paths and build a connection between preferential flow paths and hydraulic properties.

Optimization of Salt-Free Reactive Dyeing Process for Cotton with Coconut Oil/Water Dual-Phase Dyeing System

In this study, the coconut oil/water dual-phase dyeing system for developing salt-free reactive dyeing process for cotton was established. The selected dyes in this work were hot-dyeing reactive dyes, including C.I. Reactive Red 243, C.I. Reactive Blue 214 and C.I. Reactive Orange 70. A dual-phase dyeing system was performed under various coconut oil to water ratios and a comparative study was conducted on the conventional aqueous-based reactive dyeing in the presence and absence of salt. The results showed that the oil to water ratio of 3:1 imparted the highest colour yields (K/S values) and achieved a superior colour yield to the conventional aqueous-based dyeing. The results also pointed out that better dye fixation could be attained by dyeing cotton with the coconut oil/water dual-phase dyeing process under the optimum conditions. A comparable degree of dye exhaustion was observed for the aqueous-based and the dual-phase dyeing processes, however, the dye fixation was higher for the dual-phase dyeing, indicating less dye hydrolysis in this dyeing system. Consequently, superior colour fastnesses to washing and rubbing were obtained, less dye staining was observed in the case of dual-phase dyeing. A study on reusability of the coconut oil recovered from the spent dyebath for another reactive dyeing cycle was also conducted. The oil from the spent dyebath was directly taken to use without any further purification as a dual-phase medium along with water and the obtained result indicated a reusability potential of coconut oil. From this research, it infers that cleaner reactive dyeing process with the coconut oil/water dual-phase system for cotton could be developed.

Successful Technique for Closure of Macular Hole Retinal Detachment Using Autologous Retinal Transplant

Macular hole retinal detachment (MHRD) for the most part develops in highly myopic eyes. Several surgical methods have been introduced to treat MHRD. We describe our experience with the autologous retinal transplant in patient with MHRD. A 49-year-old female presented with a 2-week history of a sudden decrease in the central vision in the right eye (RE). A 3-port, 25-gauge pars plana vitrectomy was performed with the ILM dye staining and peeling. Endodiathermy was applied around a 1.5-disc diameter neurosensory donor site in the supertemporal retina. The graft was cut with standard 25-gauge curved scissors. Perfluoro-n-octane (PFO) was instilled. The free graft was gently handled until its packing into the macular hole. Two months following the initial PPV, the macular hole was closed, and vision improved from 0.05 to 0.25 logMAR.

Placental transfer of NMDAR antibodies causes reversible alterations in mice

ObjectiveTo determine whether maternofetal transfer of NMDA receptor (NMDAR) antibodies has pathogenic effects on the fetus and offspring, we developed a model of placental transfer of antibodies.MethodsPregnant C57BL/6J mice were administered via tail vein patients' or controls' immunoglobulin G (IgG) on days 14–16 of gestation, when the placenta is able to transport IgG and the immature fetal blood-brain barrier is less restrictive to IgG crossing. Immunohistochemical and DiOlistic (gene gun delivery of fluorescent dye) staining, confocal microscopy, standardized developmental and behavioral tasks, and hippocampal long-term potentiation were used to determine the antibody effects.ResultsIn brains of fetuses, patients' IgG, but not controls' IgG, bound to NMDAR, causing a decrease in NMDAR clusters and cortical plate thickness. No increase in neonatal mortality was observed, but offspring exposed in utero to patients' IgG had reduced levels of cell-surface and synaptic NMDAR, increased dendritic arborization, decreased density of mature (mushroom-shaped) spines, microglial activation, and thinning of brain cortical layers II–IV with cellular compaction. These animals also had a delay in innate reflexes and eye opening and during follow-up showed depressive-like behavior, deficits in nest building, poor motor coordination, and impaired social-spatial memory and hippocampal plasticity. Remarkably, all these paradigms progressively improved (becoming similar to those of controls) during follow-up until adulthood.ConclusionsIn this model, placental transfer of patients' NMDAR antibodies caused severe but reversible synaptic and neurodevelopmental alterations. Reversible antibody effects may contribute to the infrequent and limited number of complications described in children of patients who develop anti-NMDAR encephalitis during pregnancy.