Antimicrobial resistance of nasopharyngeal isolates of Streptococcus pneumoniae in healthy carriers: report of a study in 5-year-olds in Marcory, Abidjan, Côte d'Ivoire

ThermophilicCampylobactersare major causes of gastroenteritis in human. The main risk factor of infection is consumption of contaminated or by cross-contaminated poultry meat. In Côte d’Ivoire, gastroenteritis is usually observed but no case of human campylobacteriosis has been formally reported to date. The aims of this study were to determine prevalence and antimicrobial resistance ofCampylobacter jejuniandCampylobacter coliisolated from chickens ceaca in commercial slaughter in Abidjan. Between May and November 2009, one hundred and nineteen (119) chicken caeca samples were collected and analyzed by passive filtration method followed by molecular identification (PCR). From these 119 samples, 76 (63.8%) were positive toCampylobactertests. Among the positive colonies, 51.3% wereC. jejuniand 48.7% wereC. coli. Of the 39C. jejuniisolates, 79.5%, 38.5%, 17.9%, 10.3%, and 7.7% were, respectively, resistant, to nalidixic acid, ciprofloxacin, amoxicillin, erythromycin, and gentamicin. Among the 37 isolates ofC. coli, 78.4%, 43.2%, 13.5%, 8.1%, and 0% were resistant, respectively, to the same antibiotics. In conclusion, we reported in this study the presence of highCampylobactercontamination of the studied chickens. Molecular identification of the bacteria was performed and determination of high resistance to antimicrobials of the fluoroquinolone family was revealed.

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Antimicrobial resistance and virulence associated genes in Campylobacter jejuni isolated from chicken in Côte d’Ivoire

The Journal of Infection in Developing Countries ◽

10.3855/jidc.11355 ◽

2019 ◽

Vol 13 (08) ◽

pp. 671-677

Author(s):

Bernadette G Goualié ◽

Eric E Akpa ◽

Solange E Kakou-N’Gazoa ◽

Honoré G Ouattara ◽

Sébastien L Niamke ◽

...

Keyword(s):

Antimicrobial Resistance ◽

Campylobacter Jejuni ◽

Cote D'ivoire ◽

Côte D’Ivoire ◽

Antimicrobial Agents ◽

Diffusion Method ◽

Cote D’Ivoire ◽

Putative Gene ◽

Côte D'ivoire ◽

Microbiological Methods

Introduction: Campylobacter jejuni is one of the major causes of gastroenteritis worldwide of the last century. The aim of this study was to investigate the antibiotics profiles and the virulence gene in C. jejuni strains isolated from chicken in Côte d’Ivoire. Methodology: A total of 336 chicken ceaca samples recovered from market of two municipality of Abidjan were examined by conventional microbiological methods and molecular test using PCR. The antibiotic susceptibility tests of the isolates were determined by disk diffusion method. The presence of virulence genes was examined using simple PCR method. Results: Among of 336 samples, 168 (50%) were positives for C. jejuni. Among the C. jejuni isolates, 159 strains (94.64%) were resistant to one or more antimicrobial agents. The highest percentage of antimicrobial resistance was found for Nalidixic acid (85.33%), Tétracyclin (71.76%) and Ciprofloxacin (55.65%). Moreover, MDR including 3, 4, 5 and 6 antibiotics families was detected in 16.66% of isolates. On the other hand, detection of virulence putative gene shows presence of cadF in 100% of tested strains. In addition, cdtA, cdtB and cdtC genes were detected in 100%; 89.51% and 90.32% respectively of C. jejuniisolates. Conclusion: Because of the key role of broiler chicken in human campylobacteriosis infection, it will important in first time to monitoring using of antibiotics in chicken farms and in second time to verify presence of campylobactériosis in country.

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