Antimicrobial resistance and virulence associated genes in Campylobacter jejuni isolated from chicken in Côte d’Ivoire

Introduction: Campylobacter jejuni is one of the major causes of gastroenteritis worldwide of the last century. The aim of this study was to investigate the antibiotics profiles and the virulence gene in C. jejuni strains isolated from chicken in Côte d’Ivoire. Methodology: A total of 336 chicken ceaca samples recovered from market of two municipality of Abidjan were examined by conventional microbiological methods and molecular test using PCR. The antibiotic susceptibility tests of the isolates were determined by disk diffusion method. The presence of virulence genes was examined using simple PCR method. Results: Among of 336 samples, 168 (50%) were positives for C. jejuni. Among the C. jejuni isolates, 159 strains (94.64%) were resistant to one or more antimicrobial agents. The highest percentage of antimicrobial resistance was found for Nalidixic acid (85.33%), Tétracyclin (71.76%) and Ciprofloxacin (55.65%). Moreover, MDR including 3, 4, 5 and 6 antibiotics families was detected in 16.66% of isolates. On the other hand, detection of virulence putative gene shows presence of cadF in 100% of tested strains. In addition, cdtA, cdtB and cdtC genes were detected in 100%; 89.51% and 90.32% respectively of C. jejuniisolates. Conclusion: Because of the key role of broiler chicken in human campylobacteriosis infection, it will important in first time to monitoring using of antibiotics in chicken farms and in second time to verify presence of campylobactériosis in country.

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Antimicrobial resistance of nasopharyngeal isolates of Streptococcus pneumoniae in healthy carriers: report of a study in 5-year-olds in Marcory, Abidjan, Côte d'Ivoire

Annals of Tropical Paediatrics ◽

10.1080/02724930125253 ◽

2001 ◽

Vol 21 (2) ◽

pp. 149-154

Author(s):

A. Kacou-N'Douba, S. A. Bouzid, K. N. Guessennd, A. A. Kouassi-M'Bengue, A. Y. H. Faye-Kette, M. Dosso

Keyword(s):

Streptococcus Pneumoniae ◽

Antimicrobial Resistance ◽

Cote D'ivoire ◽

Côte D’Ivoire ◽

Cote D’Ivoire ◽

Côte D'ivoire ◽

Healthy Carriers

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Prevalence and Antimicrobial Resistance of ThermophilicCampylobacterIsolated from Chicken in Côte d’Ivoire

International Journal of Microbiology ◽

10.1155/2012/150612 ◽

2012 ◽

Vol 2012 ◽

pp. 1-5 ◽

Cited By ~ 15

Author(s):

Goualié Gblossi Bernadette ◽

Akpa Eric Essoh ◽

Kakou-N'Gazoa Elise Solange ◽

Guessennd Natalie ◽

Bakayoko Souleymane ◽

...

Keyword(s):

Antimicrobial Resistance ◽

Molecular Identification ◽

Cote D'ivoire ◽

Côte D’Ivoire ◽

Poultry Meat ◽

Campylobacter Coli ◽

Cote D’Ivoire ◽

Filtration Method ◽

Côte D'ivoire

ThermophilicCampylobactersare major causes of gastroenteritis in human. The main risk factor of infection is consumption of contaminated or by cross-contaminated poultry meat. In Côte d’Ivoire, gastroenteritis is usually observed but no case of human campylobacteriosis has been formally reported to date. The aims of this study were to determine prevalence and antimicrobial resistance ofCampylobacter jejuniandCampylobacter coliisolated from chickens ceaca in commercial slaughter in Abidjan. Between May and November 2009, one hundred and nineteen (119) chicken caeca samples were collected and analyzed by passive filtration method followed by molecular identification (PCR). From these 119 samples, 76 (63.8%) were positive toCampylobactertests. Among the positive colonies, 51.3% wereC. jejuniand 48.7% wereC. coli. Of the 39C. jejuniisolates, 79.5%, 38.5%, 17.9%, 10.3%, and 7.7% were, respectively, resistant, to nalidixic acid, ciprofloxacin, amoxicillin, erythromycin, and gentamicin. Among the 37 isolates ofC. coli, 78.4%, 43.2%, 13.5%, 8.1%, and 0% were resistant, respectively, to the same antibiotics. In conclusion, we reported in this study the presence of highCampylobactercontamination of the studied chickens. Molecular identification of the bacteria was performed and determination of high resistance to antimicrobials of the fluoroquinolone family was revealed.

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Prevalence of Staphylococcus aureus Isolated from some Street Hot Beverages in Abidjan, Côte d’Ivoire

Asian Food Science Journal ◽

10.9734/afsj/2021/v20i1130375 ◽

2021 ◽

pp. 48-55

Author(s):

Paul Yao Attien ◽

Atobla Koua ◽

Kamirou Chabi-Sika ◽

Haziz Sina ◽

Ibrahim Konate ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Cote D'ivoire ◽

Biochemical Analysis ◽

Côte D’Ivoire ◽

Egg Yolk ◽

Diffusion Method ◽

Biochemical Tests ◽

Cote D’Ivoire ◽

Gram Staining ◽

Côte D'ivoire

Introduction: Staphylococcus aureus is one of the main pathogens found in street food, including hot beverages. However, information about S. aureus isolated from street hot beverages from coffee carts is very limited in Abidjan, Côte d’Ivoire. Aims: We aimed to characterize phenotypically S. aureus isolated from street hot beverages sold in Abidjan. Methodology: A total of 400 samples of hot beverage were collected and analyzed. The identification was made through conventional microbial and biochemical analysis. Macroscopic identification on the Baird Parker agar supplement with egg yolk tellurite. Microscopic observation through Gram staining as well as biochemical tests such as catalase, DNase and coagulase were performed. To confirm staphylococcal strains, the MALDI-TOF mass spectrometry was used. After identification, the susceptibility of the staphylococcal isolates was evaluated using disc diffusion method. Results: Result showed that most of Staphylococcus aureus (18.4%) were isolated from tea. All the strains of Staphylococcus aureus isolated from street beverages were sensitive to cefoxitin and vancomycin. All the S. aureus isolated from milk were resistant to Erythromycin. Although beverages are consumed hot, the presence of S. aureus in ready-to-drink beverage transmitted infections to consumers. Conclusion: This drink need attention for the seller and the user to avoid some infections.

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First Case of Qnr B6 and Qnr B7 Genes in Enterobacteriaceae Producing Extended-Spectrum Beta-Lactamases in Abidjan, Côte D'ivoire

South Asian Journal of Research in Microbiology ◽

10.9734/sajrm/2019/v3i230082 ◽

2019 ◽

pp. 1-6

Author(s):

Gadou Victoire ◽

Toty Abalé Anatole ◽

Konan Fernique ◽

Tiékoura Konan Bertin ◽

Ouattara Mohamed Baguy ◽

...

Keyword(s):

Resistance Genes ◽

Cote D'ivoire ◽

Côte D’Ivoire ◽

Fluoroquinolone Resistance ◽

Diffusion Method ◽

Pasteur Institute ◽

Cote D’Ivoire ◽

Extended Spectrum ◽

Côte D'ivoire ◽

First Case

Aims: The aim of this study was to characterize fluoroquinolone resistance genes in enterobacteriaceae that produce extended-spectrum β-lactamases, isolated in Abidjan. Place and Duration of Study: Pasteur Institute of Côte d'Ivoire and research unit on emerging tropical infectious diseases of Aix-Marseille University from January 2017 at July 2017. Methodology: The study included 90 enterobacteriaceae producing extended-spectrum β-lactamases isolated from biological products from various hospital services in Abidjan. These strains have been pre-identified and stored at the Center for Biological Resources (CeReB) of Pasteur Institute of Côte d'Ivoire. The identification of the strains was confirmed using the mass spectrometry MALDI-TOF (MS) and the antibiotic sensitivity test was performed using Müeller Hinton's agar diffusion method. The fluoroquinolone resistant genes were detected by conventional PCR and then, sequenced. Results: The strains studied were Escherichia coli (44), Klebsiella pneumoniae (31) and Enterobacter cloacae (15). High resistance rates to ceftriaxone (96.7%), cefotaxime (95.6%), aztreonam (95.6%) and cefoxitin (72.2%) were observed in all strains producing broad spectrum β-lactamases. The resistance rate to fluororquinolones represented by ciprofloxacin was 86.7%. The fluoroquinolone resistance genes detected were qnr A (3.3%) and qnr B (42.2%). Sequencing identified the qnr A1 (3.3%), qnr B1 (31.1%), qnr B6 (2.2%) and qnr B7 (1.1%) genes. Conclusion: This study made it possible to identify fluoroquinolone resistance genes in enterobacteriaceae producing β-lactamases which have an extended spectrum in Abidjan.

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Chemical Composition and Antibacterial Activities of the Essential Oils from the Leaves of Premna angolensis and Premna quadrifolia, Two Lamiaceae from Côte d’Ivoire

Volume 5 - 2020, Issue 9 - September - International Journal of Innovative Science and Research Technology ◽

10.38124/ijisrt20aug300 ◽

2020 ◽

Vol 5 (8) ◽

pp. 681-686

Author(s):

KOUASSI Kouamé Séraphin ◽

KOUAME Bosson Antoine ◽

MAMYRBÉKOVA-BÉKRO Janat Akhanovna ◽

BEKRO YvesAlain ◽

FOFIE N’guessan Bra Yvette

Keyword(s):

Chemical Composition ◽

Cote D'ivoire ◽

Côte D’Ivoire ◽

Antibacterial Activities ◽

Antimicrobial Activities ◽

Diffusion Method ◽

Chemical Compositions ◽

Cote D’Ivoire ◽

Côte D'ivoire ◽

Germacrene D

This study was conducted with the objective of contributing to the valorization of aromatic and medicinal plants of Côte d’Ivoire. The essential oil (EO) of the leaves of two species of Premna, P. angolensis and P. quadrifolia obtained through steam distillation process, were analized through gas chromatography coupled with mass spectroscopy (GC-MS) and their antimicrobial activities was evaluated by agar diffusion method. The yields of EO extracted from the leaves of P. angolensis and P. quadrifolia are respectively 0.10 ± 0.02% and 0.40 ± 0.02%. 99.43% of the total chemical composition of the EO of P. angolensis leaves, are composed mainly of hydrocarbon sesquiterpenes (71.90%) followed by oxygenates (14.16%) and monoterpenes (13.22%). The main compounds found are β-caryophyllene (33.07%) and Humulene (10.78%). The EO of P. quadrifofia leaves, 99.59% of the composition was identified. It contains hydrocarbon sesquiterpenes (89.15%), followed by oxygenates compounds (8.61%) and monoterpenes (1.83%). The major compounds are αbulnesene (23.33%), germacrene D (18.83%) and caryophyllene (18.06%). The antimicrobial tests have shown that EO of P. quadrifolia leaves has no activites on studied strains and P. angolensis oil has a mild activity on Staphylococcus epidermidis and Klebsielle pneumoneae and a weak sensibility on Staphylococcus aureus CIP 4.83 and Candida albicans ATCC 10231. This study highlights chemical composition of the EO from P. angolensis and P. quadrifolia leaves. It shows more similarities of the chemical compositions of these two species of Premna and the interesting antimicrobial activity potential of P. angolensis EO

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Antimicrobial resistance of nasopharyngeal isolates ofStreptococcus pneumoniaein healthy carriers: report of a study in 5-year-olds in Marcory, Abidjan, Côte d'Ivoire

Annals of Tropical Paediatrics ◽

10.1080/02724930120058223 ◽

2001 ◽

Vol 21 (2) ◽

pp. 149-154 ◽

Cited By ~ 5

Author(s):

A. Kacou-N'douba ◽

S. A. Bouzid ◽

K. N. Guessennd ◽

A. A. Kouassi-M'bengue ◽

A. Y. H. Faye-Kette ◽

...

Keyword(s):

Antimicrobial Resistance ◽

Cote D'ivoire ◽

Côte D’Ivoire ◽

Cote D’Ivoire ◽

Côte D'ivoire ◽

Healthy Carriers

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Implementation of an environmental monitoring network and a pollution combating unit in Côte d'Ivoire

Water Science & Technology ◽

10.2166/wst.1995.0679 ◽

1995 ◽

Vol 32 (9-10) ◽

pp. 141-150

Author(s):

G. Morel ◽

P. Koffi Koffi

Keyword(s):

Cote D'ivoire ◽

Côte D’Ivoire ◽

Technical Assistance ◽

Monitoring Network ◽

Reference Laboratory ◽

Cote D’Ivoire ◽

Côte D'ivoire ◽

Microbiological Methods ◽

High Concentrations ◽

The Government

Aquatic pollution in Côte d'Ivoire is severe in several places. The Ivorian government therefore requested and later obtained technical assistance from the Danish government for the training of personnel and the equipment of an environmental central laboratory and of a pollution combating unit. The structure CIAPOL (Centre Ivorian Anti-Pollution) in charge of setting up the two components of the project, was created by the government of Côte d'Ivoire in May 1991. A coastal and a continental monitoring network have been implemented. Nutrients, heavy metals, pesticides, hydrocarbons and microbiological methods have been adapted for the survey programs of water, sediment and biota. Results presented in this paper show spatiotemporal variations of the ammonia concentrations in the lagoon waters. Very high concentrations in the Bietry bay of hydrocarbons in sediment and zinc in oysters trace petroleum refinery pollution. The extremely high microbiological contamination of the lagoon water around Abidjan should preclude its use for any purpose. The staff of the centre has been trained in the use of the oil spill recovery equipment. Alert procedures and communications have been introduced. Today the CIAPOL is involved in national conventions and international projects. It is designated to become the Reference Laboratory in the region and will be in charge of training and intercalibration sessions within these projects.

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