The Hitachi Graphite Furnace-Zeeman Atomic Absorption Spectrometer as an Automated, Element-Specific Detector for High Pressure Liquid Chromatography: The Separation of Arsenobetaine, Arsenocholine and Arsenite/Arsenate

1979 ◽  
Vol 6 (4) ◽  
pp. 313-319 ◽  
Author(s):  
R. A. Stockton ◽  
Kurt J. Irgolic
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Atomic Absorption ◽  
High Pressure Liquid Chromatography ◽  
Graphite Furnace ◽  
Atomic Absorption Spectrometer ◽  
Specific Detector ◽  
Absorption Spectrometer

Separation and Detection of Arsenical Pesticide Residues and Some of Their Metabolites by High Pressure Liquid Chromatography-Graphite Furnace Atomic Absorption Spectrometry

1980 ◽  
Vol 63 (3) ◽  
pp. 523-528 ◽  
Author(s):  
Edwin A Woolson ◽  
Nadav Aharonson
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Atomic Absorption ◽  
High Pressure Liquid Chromatography ◽  
Limit Of Detection ◽  
Graphite Furnace ◽  
Dimethylarsinic Acid ◽  
Anion Exchange Column ◽  
Soil Extracts ◽  
Graphite Furnace Atomic Absorption

Abstract A method was developed for the separation and quantitative determination of arsenite, methanearsonic acid (MAA), dimethylarsinic acid (cacodylic acid or CA), and arsenate by high pressure liquid chromatography (HPLC) coupled to a graphite furnace atomic absorption (GFAA) detector. The compounds are separated on a low capacity anion exchange column with gradient elution from 100+ water-methanol (80 + 20, v/v) to 100% 0.02M (NH4)2CO3-methanol (85 + IS, v/v) at 1.2 mL/min and a pressure of about 300 psi. The HPLC was coupled by an automatic sampling unit to the GFAA through an interface consisting of a Teflon flow-through sampling cup. Limit of detection for the arsenic compounds was about 5 ng As injected in aliquots of 20 μL (0.25 ppm As in solution). All 4 arsenical compounds gave similar calibration curves, which were linear from 0.1 to 2.0 ng for single atomizations in the GFAA and from 5 to 200 ng when multiple, sequential atomizations of HPLC eluant were used to quantitate HPLC peaks. Quenching by soil extracts as well as aqueous HPLC solvents was minimal.

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