Nucleotide sequence analysis of a C1 gene fragment of psittacine beak and feather disease virus amplified by real-time polymerase chain reaction indicates a possible existence of genotypes

2004 ◽  
Vol 33 (1) ◽  
pp. 41-50 ◽  
Author(s):  
Rüdiger Raue ◽  
Reimar Johne ◽  
Lorenzo Crosta ◽  
Marcellus Bürkle ◽  
Helga Gerlach ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Sequence Analysis ◽  
Nucleotide Sequence ◽  
Real Time ◽  
Disease Virus ◽  
Nucleotide Sequence Analysis ◽  
Gene Fragment ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Feather Disease Virus

A quantitative, real-time polymerase chain reaction assay for beak and feather disease virus

2009 ◽  
Vol 159 (1) ◽  
pp. 98-104 ◽  
Author(s):  
Patrick L. Shearer ◽  
Margaret Sharp ◽  
Nicolai Bonne ◽  
Phillip Clark ◽  
Shane R. Raidal
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Disease Virus ◽  
Polymerase Chain Reaction Assay ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Feather Disease Virus

scholarly journals Psittacine beak and feather disease virus in budgerigars and ring-neck parakeets in South Africa

2004 ◽  
Vol 71 (1) ◽  
Author(s):  
J. Albertyn ◽  
K.M. Tajbhai ◽  
R.R. Bragg
Keyword(s):  
South Africa ◽  
Polymerase Chain Reaction ◽  
Genetic Variation ◽  
Disease Virus ◽  
Common Disease ◽  
Sample Collection ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Feather Disease Virus ◽  
Viral Isolates

Psittacine beak and feather disease (PBFD) is a common disease of the psittacine species and is caused by the psittacine beak and feather disease virus (PBFDV). In this study the occurrence of the disease in ring-neck parakeets and budgerigars in South Africa suffering from feathering problems, using polymerase chain reaction as a diagnostic test was investigated. The genetic variation between viral isolates was also studied. Results indicate that PBFDV can be attributed to being the cause of feathering problems in some of the ring-neck parakeets and budgerigars in South Africa. Genetic variation of isolates occurs between species and individuals. A cheap and easy to use method of blood sample collection on filter paper for diagnostic purposes was also evaluated. It proved to be less stressful to the birds and did not inhibit further processes.

scholarly journals Polymerase Chain Reaction Assays for the Detection and Discrimination of the Soybean Rust Pathogens Phakopsora pachyrhizi and P. meibomiae

2002 ◽  
Vol 92 (2) ◽  
pp. 217-227 ◽  
Author(s):  
Reid D. Frederick ◽  
Christine L. Snyder ◽  
Gary L. Peterson ◽  
Morris R. Bonde
Keyword(s):  
Polymerase Chain Reaction ◽  
Nucleotide Sequence ◽  
Real Time ◽  
Sequence Similarity ◽  
Morphological Characteristics ◽  
Its Region ◽  
Soybean Rust ◽  
Phakopsora Pachyrhizi ◽  
Chain Reaction ◽  
Polymerase Chain

Soybean rust occurs in Australia and many countries throughout Africa, Asia, and South America. The causal agents of soybean rust are two closely related fungi, Phakopsora pachyrhizi and P. meibomiae, which are differentiated based upon morphological characteristics of the telia. Determination of the nucleotide sequence of the internal transcribed spacer (ITS) region revealed greater than 99% nucleotide sequence similarity among isolates of either P. pachyrhizi or P. meibomiae, but only 80% sequence similarity between the two species. Utilizing differences within the ITS region, four sets of polymerase chain reaction (PCR) primers were designed specifically for P. pachyrhizi and two sets for P. meibomiae. Classical and real-time fluorescent PCR assays were developed to identify and differentiate between P. pachyrhizi and P. meibomiae. Identification of P. pachyrhizi from infected soybean leaves using the real-time PCR assay will allow for more rapid diagnoses.

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