Assessment of In-vivo Toxicity of Composite Materials Based on Poly-L-lactic Acid andβ-Tricalcium Phosphate

Biodegradable scaffolds based on biomedical polymeric materials have attracted wide interest in bone transplantation for clinical treatment for bone defects without a second operation. The composite materials of poly(trimethylene carbonate), poly(L-lactic acid), and hydroxyapatite (PTMC/PLA/HA and PTMC/HA) were prepared by the modification and blending of PTMC with PLA and HA, respectively. The PTMC/PLA/HA and PTMC/HA scaffolds were further prepared by additive manufacturing using the biological 3D printing method using the PTMC/PLA/HA and PTMC/HA composite materials, respectively. These scaffolds were also characterized by Fourier transform infrared spectroscopy (FT-IR), gel permeation chromatography (GPC), automatic contact-angle, scanning electronic micrographs (SEM), diffraction of X-rays (XRD), differential scanning calorimetry (DSC), and thermogravimetry (TG). Subsequently, their properties, such as mechanical, biodegradation, cell cytotoxicity, cell compatibility in vitro, and proliferation/differentiation assay in vivo, were also investigated. Experiment results indicated that PTMC/PLA/HA and PTMC/HA scaffolds possessed low toxicity, good biodegradability, and good biocompatibility and then enhanced the cell multiplication ability of osteoblast cells (MC3T3-E1). Moreover, PTMC/PLA/HA and PTMC/HA scaffolds enhanced the adhesion and proliferation of MC3T3-E1 cells and enabled the bone cell proliferation and induction of bone tissue formation. Therefore, these composite materials can be used as potential biomaterials for bone repatriation and tissue engineering.

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Biodegradable 3D Printed Scaffolds of Modified Poly(trimethylene carbonate) Composite Materials with Poly(L-lactic acid) and Hydroxyapatite for Bone Regeneration

10.22541/au.163255822.29387484/v1 ◽

2021 ◽

Author(s):

Honglei Kang ◽

Jiangxu Dong ◽

Zhi Wei Liu ◽

Shengmin Zhang ◽

Fan Liu ◽

...

Keyword(s):

Lactic Acid ◽

Composite Materials ◽

Bone Regeneration ◽

Bone Cells ◽

Polymeric Materials ◽

Host Cells ◽

Bone Tissue Regeneration ◽

Trimethylene Carbonate ◽

Biodegradable Scaffolds

The biodegradable scaffolds based on biomedical polymeric materials have attracted the wide interest in bone transplantation for clinical treatment to bone defects without the second operation. The composite materials of poly(trimethylene carbonate), poly(L-lactic acid) and hydroxyapatite (PTMC/PLA/HA) were prepared by the modification and blending of poly(trimethylene carbonate) (PTMC) with poly(L-lactic acid) (PLA) and hydroxyapatite nanoparticles (HA). The PTMC/PLA/HA scaffolds were further prepared by additive manufacturing using the biological 3D printing method. The PTMC/PLA/HA composite materials and scaffolds were also characterized by fourier transform infrared spectroscopy (FT-IR), GPC, automatic contact-angle, SEM, XRD, differential scanning calorimetry (DSC) and thermogravimetry (TG). Subsequently, their properties, such as the mechanic, biodegradation, drug controlled release, cell cytotoxicity, cell compatibility in vitro and proliferation/differentiation assay in vivo, were also investigated. Experiment results indicated that PTMC/PLA/HA scaffolds possessed the low toxicity, good biodegradability and good biocompatibility and then improved the cell proliferation of osteoblast cells (MC3T3-E1). Moreover, PTMC/PLA/HA enhanced the adhesion and proliferation of MC3T3-E1 cells, allowing penetration of host cells within the construct boundaries, and then can enable the growing of bone cells and induction of bone tissue regeneration. Therefore, PTMC/PLA/HA composite materials can be used as the potential biodegradable scaffolds for bone regeneration and tissue engineering in vivo.

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Poly-L-Lactic Acid β-Tricalcium Phosphate Screws: A Preliminary In Vivo Biocompatibility Study

International Journal of Immunopathology and Pharmacology ◽

10.1177/039463200702000126 ◽

2007 ◽

Vol 20 (1) ◽

pp. 207-211 ◽

Cited By ~ 8

Author(s):

N. Magarelli ◽

M.A. Savastano ◽

D. Palmieri ◽

R. Zappacosta ◽

G. Lattanzio ◽

...

Keyword(s):

Lactic Acid ◽

Tricalcium Phosphate ◽

Biocompatibility Study

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Evaluation of In-Vitro Cytotoxicity of Composite Materials Composed of Poly-L-lactic Acid and β-Tricalcium Phosphate

Polymer-Plastics Technology and Engineering ◽

10.1080/03602550903532133 ◽

2010 ◽

Vol 49 (4) ◽

pp. 381-386 ◽

Cited By ~ 10

Author(s):

Zeqiang He ◽

Lizhi Xiong

Keyword(s):

Lactic Acid ◽

Composite Materials ◽

Tricalcium Phosphate ◽

In Vitro Cytotoxicity

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NT4X-167; Novel N-terminal Aβ specific antibody, prevents in vitro and in vivo toxicity of highly toxic Aβ4-42 species

Pharmacopsychiatry ◽

10.1055/s-0035-1558042 ◽

2015 ◽

Vol 48 (06) ◽

Author(s):

G Antonios ◽

H Borgers ◽

T Pilot ◽

V Pena ◽

T Bayer

Keyword(s):

Specific Antibody ◽

In Vivo Toxicity

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Negative cross resistance of Spodoptera litura Fabricius population of tomato to newer molecule spinetoram

ENTOMON ◽

10.33307/entomon.v44i2.439 ◽

2019 ◽

Vol 44 (2) ◽

pp. 127-132

Author(s):

M. Visnupriya ◽

N. Muthukrishnan

Keyword(s):

Spodoptera Litura ◽

Laboratory Strain ◽

Field Population ◽

Resistance Ratio ◽

Cross Resistance ◽

In Vivo Toxicity

Field population of Spodoptera litura from tomato ( resistant to the majority of the conventional insecticide molecules) were subjected to the in vivo toxicity of spinetoram 12 SC to assess whether cross resistance exists or not. Untreated larvae of both field and laboratory strains showed no mortality during 48 hours of feeding. After 48 hours of feeding on spinetoram 12 SC treated leaves, LC50s of field larvae were 0.28, 0.93, 3.71 and 7.11 ppm for the 2nd, 3rd, 4th and 5th instars of S. litura respectively. However, in the laboratory strain these values were 1.12, 5.86, 36.72 and 91.55 ppm for 2nd, 3rd, 4th and 5th instars of S. litura respectively. Resistance ratio was 0.25, 0.16, 0.10 and 0.08 for the 2nd instar up to the 5th instar of S. litura.

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