Variation in electrophoretic karyotype among Mexican isolates ofMycosphaerella fijiensis

2006 ◽  
Vol 28 (2) ◽  
pp. 236-241 ◽  
Author(s):  
C. M. Rodríguez-García ◽  
N. Raigosa-Flores ◽  
L. Conde-Ferráez ◽  
L. Peraza-Echeverría ◽  
B. Canto-Canché ◽  
...  
Keyword(s):  
Electrophoretic Karyotype

Assignment of cloned genes to the seven electrophoretically separated Candida albicans chromosomes

1988 ◽  
Vol 8 (11) ◽  
pp. 4721-4726
Author(s):  
B B Magee ◽  
Y Koltin ◽  
J A Gorman ◽  
P T Magee
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Candida Albicans ◽  
Unknown Function ◽  
Gel Electrophoresis ◽  
Homogeneous Field ◽  
Electrophoretic Karyotype ◽  
Chromosome Size ◽  
Homologous Chromosomes ◽  
Orthogonal Field ◽  
Chromosomal Bands

By using orthogonal-field alternating gel electrophoresis (OFAGE), field-inversion gel electrophoresis (FIGE), and contour-clamped homogeneous field gel electrophoresis (CHEF), we have clearly resolved 11 chromosomal bands from various Candida albicans strains. OFAGE resolves the smaller chromosomes better, while FIGE, which under our conditions causes the chromosomes to run in the reverse order of OFAGE, is more effective in separating the larger chromosomes. CHEF separates all chromosomes under some conditions, but these conditions do not often resolve homologs. The strains examined are highly polymorphic for chromosome size. Fourteen cloned Candida genes, isolated on the basis of conferral of new properties to or complementation of auxotrophic deficiencies in Saccharomyces cerevisiae, and three sequences of unknown function have been hybridized to Southern transfers of CHEF, FIGE, and OFAGE gels. Four sets of resolvable bands have been shown to be homologous chromosomes. On the basis of these data, we suggest that C. albicans has seven chromosomes. Genes have been assigned to the seven chromosomes. Two chromosomes identified genetically have been located on the electrophoretic karyotype.

scholarly journals An electrophoretic karyotype of Neurospora crassa.

1988 ◽  
Vol 8 (4) ◽  
pp. 1469-1473 ◽  
Author(s):  
M J Orbach ◽  
D Vollrath ◽  
R W Davis ◽  
C Yanofsky
Keyword(s):  
Neurospora Crassa ◽  
Electric Fields ◽  
Gel Electrophoresis ◽  
Electrophoretic Karyotype ◽  
Dna Molecules ◽  
Linkage Groups ◽  
Homogeneous Electric Field ◽  
Chromosomal Dna ◽  
Molecular Karyotype ◽  
Electrophoresis System

A molecular karyotype of Neurospora crassa was obtained by using an alternating-field gel electrophoresis system which employs contour-clamped homogeneous electric fields. The migration of all seven N. crassa chromosomal DNAs was defined, and five of the seven molecules were separated from one another. The estimated sizes of these molecules, based on their migration relative to Schizosaccharomyces pombe chromosomal DNA molecules, are 4 to 12.6 megabases. The seven linkage groups were correlated with specific chromosomal DNA bands by hybridizing transfers of contour-clamped homogeneous electric field gels with radioactive probes specific to each linkage group. The mobilities of minichromosomal DNAs generated from translocation strains were also examined. The methods used for preparation of chromosomal DNA molecules and the conditions for their separation should be applicable to other filamentous fungi.

Electrophoretic karyotype analysis of Botrytis cinerea

1993 ◽  
Vol 99 (S3) ◽  
pp. 119-128 ◽  
Author(s):  
J. A. L. Kan ◽  
A. Goverse ◽  
C. J. B. Vlugt-Bergmans
Keyword(s):  
Botrytis Cinerea ◽  
Karyotype Analysis ◽  
Electrophoretic Karyotype

Complementary mating types of Mucor circinelloides show electrophoretic karyotype heterogeneity

1999 ◽  
Vol 36 (6) ◽  
pp. 383-389 ◽  
Author(s):  
José María Díaz-Mínguez ◽  
M. Angeles López-Matas ◽  
Arturo P. Eslava
Keyword(s):  
Mucor Circinelloides ◽  
Electrophoretic Karyotype ◽  
Mating Types ◽  
Karyotype Heterogeneity

Electrophoretic karyotype of the astaxanthin-producing yeast Phaffia rhodozyma

1995 ◽  
Vol 27 (5) ◽  
pp. 447-450 ◽  
Author(s):  
Jos� L. Adrio ◽  
Mercedes L�pez ◽  
Javier Casqueiro ◽  
Concepci�n Fern�ndez ◽  
Manuel Veiga
Keyword(s):  
Phaffia Rhodozyma ◽  
Electrophoretic Karyotype

scholarly journals Electrophoretic karyotype of Fusarium solani

Microbiology ◽  
1994 ◽  
Vol 140 (6) ◽  
pp. 1373-1375 ◽  
Author(s):  
S. W. Nazareth ◽  
C. V. Bruschi
Keyword(s):  
Fusarium Solani ◽  
Electrophoretic Karyotype

scholarly journals Kazachstania aquatica sp. nov. and Kazachstania solicola sp. nov., novel ascomycetous yeast species

2005 ◽  
Vol 55 (5) ◽  
pp. 2219-2224 ◽  
Author(s):  
Zuo-Wei Wu ◽  
Feng-Yan Bai
Keyword(s):  
Yeast Species ◽  
Novel Species ◽  
Its Region ◽  
Culture Collection ◽  
Electrophoretic Karyotype ◽  
Ascomycetous Yeast ◽  
Centraalbureau Voor ◽  
Domain Sequence ◽  
D2 Domain ◽  
Beijing China

The unidentified strains AS 2.0706T, preserved in the China General Microbiological Culture Collection Center (CGMCC), Academia Sinica, Beijing, China, and CBS 6904T, preserved in the Centraalbureau voor Schimmelcultures (CBS), Utrecht, The Netherlands, were shown to represent two novel ascomycetous yeast species of the genus Kazachstania by 18S rDNA, internal transcribed spacer (ITS) region (including 5·8S rDNA) and 26S rDNA D1/D2 domain sequence analysis and electrophoretic karyotype comparison. The names Kazachstania aquatica sp. nov. and Kazachstania solicola sp. nov. are proposed for strains AS 2.0706T and CBS 6904T, respectively. Phylogenetically, the two novel species are closely related to Kazachstania aerobia, Kazachstania servazzii and Kazachstania unispora.

Sign in / Sign up

Export Citation Format

Share Document