Transferrin binding protein-B from Neisseria meningitidis C as a novel carrier protein in glycoconjugate preparation: an in silico approach

2021 ◽  
pp. 1-11
Author(s):  
Abhijeet Karale ◽  
Kiran Bharat Lokhande ◽  
Niraj Shende ◽  
K. V. Swamy ◽  
Rajeev Dhere ◽  
...  
Keyword(s):  
Neisseria Meningitidis ◽  
In Silico ◽  
Binding Protein ◽  
Carrier Protein ◽  
Protein B

scholarly journals Transferrin-Binding Protein B of Neisseria meningitidis: Sequence-Based Identification of the Transferrin-Binding Site Confirmed by Site-Directed Mutagenesis

2004 ◽  
Vol 186 (3) ◽  
pp. 850-857 ◽  
Author(s):  
Geneviève Renauld-Mongénie ◽  
Laurence Lins ◽  
Tino Krell ◽  
Laure Laffly ◽  
Michèle Mignon ◽  
...  
Keyword(s):  
Neisseria Meningitidis ◽  
Binding Protein ◽  
Prediction Method ◽  
Binding Activity ◽  
Site Directed Mutagenesis ◽  
Titration Calorimetry ◽  
Directed Mutagenesis ◽  
Binding Domains ◽  
Homologous Strain ◽  
Protein B

ABSTRACT A sequence-based prediction method was employed to identify three ligand-binding domains in transferrin-binding protein B (TbpB) of Neisseria meningitidis strain B16B6. Site-directed mutagenesis of residues located in these domains has led to the identification of two domains, amino acids 53 to 57 and 240 to 245, which are involved in binding to human transferrin (htf). These two domains are conserved in an alignment of different TbpB sequences from N. meningitidis and Neisseria gonorrhoeae, indicating a general functional role of the domains. Western blot analysis and BIAcore and isothermal titration calorimetry experiments demonstrated that site-directed mutations in both binding domains led to a decrease or abolition of htf binding. Analysis of mutated proteins by circular dichroism did not provide any evidence for structural alterations due to the amino acid replacements. The TbpB mutant R243N was devoid of any htf-binding activity, and antibodies elicited by the mutant showed strong bactericidal activity against the homologous strain, as well as against several heterologous tbpB isotype I strains.

scholarly journals Delineating the regions of human transferrin involved in interactions with transferrin binding protein B from Neisseria meningitidis

2010 ◽  
Vol 77 (5) ◽  
pp. 1301-1314 ◽  
Author(s):  
Jessmi M. L. Ling ◽  
Collin H. Shima ◽  
David C. Schriemer ◽  
Anthony B. Schryvers
Keyword(s):  
Neisseria Meningitidis ◽  
Binding Protein ◽  
Human Transferrin ◽  
Protein B

In silico Screening and Molecular Interaction Studies of Tetrahydrocannabinol and its Derivatives with Acetylcholine Binding Protein

2018 ◽  
Vol 12 (2) ◽  
pp. 181-190 ◽  
Author(s):  
Priya P. Panigrahi ◽  
Ramit Singla ◽  
Ankush Bansal ◽  
Moacyr Comar Junior ◽  
Vikas Jaitak ◽  
...  
Keyword(s):  
Molecular Interaction ◽  
In Silico ◽  
Binding Protein ◽  
In Silico Screening ◽  
Acetylcholine Binding Protein ◽  
Interaction Studies
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