scholarly journals Serosurvey of Chlamydia psittaci Infections among Livestock and Human Beings Employing Complement Fixation Test

1997 ◽  
Vol 11 (2) ◽  
pp. 163-168 ◽  
Author(s):  
V. B. Joshi ◽  
R. C. Katoch ◽  
K. B. Nagal ◽  
M. K. Batta ◽  
R. K. Asrani ◽  
...  
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Chlamydia Psittaci ◽  
Fixation Test ◽  
Human Beings

scholarly journals A study of human respiratory tract chlamydial infections in Cambridgeshire 1986–88

1990 ◽  
Vol 104 (3) ◽  
pp. 479-488 ◽  
Author(s):  
T. G Wreghitt ◽  
C. E Barker ◽  
J. D Treharne ◽  
J. M Phipps ◽  
V Robinson ◽  
...  
Keyword(s):  
Respiratory Tract ◽  
Chlamydia Pneumoniae ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Chlamydia Psittaci ◽  
Fixation Test ◽  
Serum Samples ◽  
Human Respiratory Tract ◽  
Relative Incidence ◽  
Chlamydial Infections

SUMMARYHuman respiratory tract chlamydial infections have been studied in Cambridge-shire for many years, but until recently we have been unable to distinguish between infection withChlamydia psittaciOrChlamydia pneumoniae(TWAR). In this study, we have employed the micro-immunofluorescence (micro-IF) test for this purpose and to look for the relative incidence ofC. psittaciandC. pneumoniaeinfections in Cambridgeshire. Among 50 patients with community-acquired respiratory tract symptoms whose serum samples had Chlamydia complement fixation test titres ≥ 64, 25 had evidence of recentC. psittaciorC. pneumoniaeinfection. Nineteen (76%) of the 25 patients had evidence of recentC. psittaciinfection and of these 16 (84%) had recently had contact with birds. Six patients (24%) had evidence of recentC. pneumoniaeinfection, and of these, only two (33% had recently had contact with birds). WhileC. psittaciwas grown from several of the birds associated with humanC. psittaciinfection, it was not cultured from any of the birds in contact with the two humanC. pnemoniaecases.

scholarly journals IMMUNITY IN MUMPS

1945 ◽  
Vol 81 (1) ◽  
pp. 119-135 ◽  
Author(s):  
John F. Enders ◽  
Sidney Cohen ◽  
Lewis W. Kane
Keyword(s):  
Skin Test ◽  
Specific Antibody ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Mumps Virus ◽  
Fixation Test ◽  
The Other ◽  
Human Beings ◽  
Inapparent Infection

1. A specific antibody, demonstrable by the technique of complement fixation, regularly appears, or increases in concentration, in the sera of human beings during an attack of mumps or during convalescence. 2. Specific dermal hypersensitivity, demonstrable by the injection of heat-inactivated mumps virus, has been shown to develop in 6 human beings after recovery from mumps. 3. Complement-fixing antibody and the hypersensitive state also emerge as a result of clinically inapparent infection with the virus of mumps. 4. These two phenomena are apparently unrelated in respect to immunologic mechanisms. 5. The data presented indicate that the complement fixation test should prove of value both in diagnosis and in the determination of immunity. 6. The skin test for dermal hypersensitivity, on the other hand, becomes positive after recovery and therefore would appear to be useful only as an index of resistance.

Evaluation of the Complement Fixation Test in Amebiasis

1952 ◽  
Vol 21 (3) ◽  
pp. 391-399 ◽  
Author(s):  
Elwood Buchman ◽  
Harold J. Kullman ◽  
George F. Margonis
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test

IMMUNOASSAY OF GONADOTROPHINS USING COMPLEMENT FIXATION

1969 ◽  
Vol 62 (1_Suppl) ◽  
pp. S113-S133 ◽  
Author(s):  
Sam Brody
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Research Work ◽  
Fixation Test ◽  
Years Of Experience ◽  
Standard Practice ◽  
Clinical Observations ◽  
Technical Procedure ◽  
Methodological Problems

ABSTRACT This report is a summary of 10 years of experience with the complement fixation test as adopted for the immunoassay of HCG in serum. It is based on published as well as unpublished material. The discussion centers mainly around methodological problems, criteria of reliability, and clinical observations. It is our impression that the complement fixation test is a reasonably rapid and simple technical procedure. It is standard practice in every bacteriological and virological laboratory. The precision of the HCG assay is high. Its accuracy is good. The complement fixation assay, as reported here, fulfils the criteria of specificity. It has been evaluated by means of serological techniques and through comparison between biopotency and immunopotency of HCG in serum with reference to a common standard. Its application for routine as well as research work is illustrated.

Rapid detection of Chlamydia/Chlamydophila group in samples collected from swine herds with and without reproductive disorders

2014 ◽  
Vol 17 (2) ◽  
pp. 367-369 ◽  
Author(s):  
K. Rypula ◽  
A. Kumala ◽  
P. Lis ◽  
K. Niemczuk ◽  
K. Płoneczka-Janeczko ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
Reproductive Disorders ◽  
Serum Samples ◽  
Swine Herds

Abstract The study was carried out in seven reproductive herds of pigs. In three of them reproductive disorders were observed. Three herds consisted of 10-50 and four consisted of 120-500 adult sows and they were called small and medium, respectively. Fifty-seven adult sows were randomly selected from herds. Serum samples were tested using the complement fixation test and swabs from both eyes and from the vaginal vestibule were examined using real-time PCR. All serum samples were negative. Infected sows were present in each of the study herds. In total, there were 28 positive samples (53%, 28/48) in real-time PCR in sows with reproductive disorders and 35 (53%, 35/66) in sows selected from herds without problems in reproduction. One isolate proved to be Chlamydophila pecorum, whereas all the remaining were Chamydia suis

Assessment of a complement fixation test to detect Mycoplasma hyopneumoniae infection in pigs

1984 ◽  
Vol 61 (7) ◽  
pp. 216-218
Author(s):  
L. C. LLOYD ◽  
R. T. BADMAN ◽  
J. R. ETHERIDGE ◽  
K. McKECHNIE ◽  
H. IYER
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Mycoplasma Hyopneumoniae ◽  
Fixation Test

Effect of Calcium Ion on the Kolmer Complement-Fixation Test

1954 ◽  
Vol 24 (8) ◽  
pp. 934-945 ◽  
Author(s):  
Alcor S. Browne ◽  
Martha M. Michelbacher ◽  
Edith M. Coffey
Keyword(s):  
Calcium Ion ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test

scholarly journals Brucella abortus RB51 and Hot Saline Extract from Brucella ovis as Antigens in a Complement Fixation Test Used To Detect Sheep Vaccinated with Brucella abortus RB51

2001 ◽  
Vol 8 (1) ◽  
pp. 119-122 ◽  
Author(s):  
Rosanna Adone ◽  
Franco Ciuchini
Keyword(s):  
Immune Responses ◽  
Brucella Abortus ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Infected Sheep ◽  
Fixation Test ◽  
Anticomplementary Activity ◽  
Serum Samples ◽  
Saline Extract ◽  
Brucella Ovis

ABSTRACT The efficacy of Brucella abortus RB51 and hot saline extract (HSE) from Brucella ovis as antigens in complement fixation (CF) tests was comparatively evaluated in detecting immune responses of sheep vaccinated with B. abortus strain RB51. For this study, four 5-month-old sheep were vaccinated subcutaneously with 5 × 109 CFU of RB51, and two sheep received saline. Serum samples collected at different times after vaccination were tested for the presence of antibodies to RB51 by a CF test with RB51 as antigen, previously deprived of anticomplementary activity, and with HSE antigen, which already used as the official antigen to detectB. ovis-infected sheep. The results showed that vaccinated sheep developed antibodies which reacted weakly against HSE antigen and these antibodies were detectable for 30 days after vaccination. However, antibodies to RB51 could be detected for a longer period after vaccination by using homologous RB51 antigen in CF tests. In fact, high titers were still present at 110 days postvaccination with RB51 antigen. Sera from sheep naturally infected with B. ovisalso reacted to RB51 but gave lower titers than those detected by HSE antigen. As expected, all sera from RB51-vaccinated sheep remained negative when tested with standard S-type Brucella standard antigens.

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