Determination of Organophosphorus Pesticide Residues in Carrot Using Gel Permeation Chromatography

The 10 organophosphorus pesticides in fish can be rapidly extracted and separated by gel permeation chromatography, and the lipin and organophosphorus pesticides of fish can be seperated. The interfering matters can be well eliminated in this condition, and analysis is more rapid than other methods. The residues of 10 organophosphorus pesticides at same time can be identified and quantified simultaneously by GC-PFPD. This method is simple, rapid, and the average recovery ratios were 71.7%-80.9%.Good repeatability was obtained in all the cases with relative standard deviations (RSDs) lower 11%, and limit of detection was0.0003-0.004 mg·kg-1.

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Comparison of Gel Permeation Chromatography, Sweep Codistillation, and Florisil Column Adsorption Chromatography as Sample Cleanup Techniques for the Determination of Organochlorine Pesticide Residues in Animal Fats

Journal of AOAC International ◽

10.1093/jaoac/76.6.1317 ◽

1993 ◽

Vol 76 (6) ◽

pp. 1317-1322 ◽

Cited By ~ 14

Author(s):

Paul Armishaw ◽

Roderick G Millar

Keyword(s):

Pesticide Residues ◽

Gel Permeation Chromatography ◽

Adsorption Chromatography ◽

Florisil Column ◽

Animal Fats ◽

Animal Fat ◽

Column Adsorption ◽

Gel Permeation ◽

Organochlorine Pesticide Residues

Abstract Methods using a commercial sweep codistillation apparatus, gel permeation chromatography (GPC), and Florisil column adsorption chromatography were compared as cleanup techniques for the determination of organochlorine pesticide residues in animal fats by Megabore capillary gas chromatography with electron capture detection. Animal fat that had been previously found to contain no detectable organochlorine residues was spiked with 17 organochlorine pesticides at levels ranging from 0.1 to 0.4 mg/kg and cleaned up by each of the 3 techniques. Recoveries obtained for all 3 methods were in the range 73-113%, with coefficients of variation between 1.1 and 11.2%. Equivalence of method performance was further demonstrated by performing replicate analyses of beef and sheep fat containing naturally incurred residues of heptachlor epoxide, dieldrin, p,p′-DDE, p,p′-DDD and p,p′-DDT. All 3 methods offer effective cleanup and acceptable recovery of organochlorine pesticides in animal fat. The sweep codistillation method has the advantages of low solvent and reagent use, simultaneous cleanup of 10 samples, and rapid turnaround, although thermal degradation of p,p′-DDT requires monitoring and control. GPC offers a high degree of automation but is a relatively slow sequential sample cleanup with high solvent use. Florisil column adsorption chromatography is a simple, proven technique but requires considerable solvent and reagent and has a low potential for automation.

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Determination of Organophosphorus Pesticide Residues in Milk

Bulletin of the Veterinary Institute in Pulawy ◽

10.2478/bvip-2013-0034 ◽

2013 ◽

Vol 57 (2) ◽

pp. 185-189 ◽

Cited By ~ 5

Author(s):

Tomasz Kiljanek ◽

Alicja Niewiadowska ◽

Stanisław Semeniuk

Keyword(s):

Pesticide Residues ◽

Solid Phase ◽

Organophosphorus Pesticides ◽

Organophosphorus Pesticide ◽

Gel Permeation Chromatography ◽

Proficiency Tests ◽

The European Union ◽

Photometric Detection ◽

Milk Samples

Abstract A method for simultaneous determination of 13 organophosphorus pesticide residues in milk samples has been developed and validated. The method is based on the extraction of the sample with acetone and petroleum ether, cleanup by gel permeation chromatography, and solid phase extraction, and determination by gas chromatography with flame photometric detection. The recovery, investigated by analysing blank milk samples spiked with azinphos, chlorfenvinphos, chlorpyrifos, chlorpyrifos-methyl, diazinon, fenitrothion, methacrifos, methidathion, parathion-methyl, pyrazophos, and triazophos at concentrations of 10, 20, and 30 μg/kg, and with parathion and pirimiphos-methyl at concentrations of 25, 50, and 75 μg/kg, ranged from 73.1% to 106.2%. Performance characteristics, such as repeatability and within-laboratory reproducibility expressed as coefficient of variation were less than 20%. Measurement uncertainty was lower than 22% for all validated organophosphorus pesticides. The limits of quantification were 10 μg/kg for all compounds and allowed determination of residues at, or even below, the maximum residue limits set by the European Union. The satisfactory z-score results of international proficiency tests confirm good accuracy, reproducibility, and reliability of the developed method.

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