Development of a Normal Human Immunoglobulin G Standard Curve for Enzyme‐Linked Immunosorbent Assay: Use for Comparison of Antigen Efficacy

2006 ◽  
Vol 27 (2) ◽  
pp. 173-181 ◽  
Author(s):  
Christina M. Scheel ◽  
Sukwan Handali ◽  
Edneia C. Bueno ◽  
Azra Khan ◽  
Kathy Hancock ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Immunoglobulin G ◽  
Human Immunoglobulin ◽  
Enzyme Linked Immunosorbent Assay ◽  
Human Immunoglobulin G ◽  
Standard Curve ◽  
Normal Human

scholarly journals Ability of Staphylococcus aureus Cells to Bind Normal Human Immunoglobulin G

1970 ◽  
Vol 2 (3) ◽  
pp. 342-344 ◽  
Author(s):  
R. Virgilio A. ◽  
C. González P. ◽  
Nubia Munoz S. ◽  
Silvia Mendoza G.
Keyword(s):  
Staphylococcus Aureus ◽  
Immunoglobulin G ◽  
Human Immunoglobulin ◽  
Human Immunoglobulin G ◽  
Normal Human

Concomitant but Not Causal Association Between Surface Charge and Inhibition of Phagocytosis by Cryptococcal Polysaccharide

1980 ◽  
Vol 29 (2) ◽  
pp. 295-300 ◽  
Author(s):  
Thomas R. Kozel ◽  
Errol Reiss ◽  
Robert Cherniak
Keyword(s):  
Surface Charge ◽  
Immunoglobulin G ◽  
Capsular Polysaccharide ◽  
Human Immunoglobulin ◽  
Phase System ◽  
Encapsulated Cells ◽  
Human Immunoglobulin G ◽  
Negative Surface ◽  
Normal Human ◽  
Negative Surface Charge

The mechanism by which capsular polysaccharides inhibit phagocytosis is not clearly understood. We investigated the association between a negative surface charge and inhibition of phagocytosis by the capsular polysaccharide of Cryptococcus neoformans . A two-polymer aqueous-phase system containing phosphate ions was used to assess surface charge. Opsonins such as normal bovine serum and normal human immunoglobulin G reduced the surface charge on non-encapsulated cryptococci and simultaneously enhanced phagocytosis. These same opsonins had no effect on phagocytosis or surface charge of encapsulated cryptococci. F (ab′) 2 fragments of normal human immunoglobulin G neither enhanced phagocytosis nor altered the surface charge of non-encapsulated cryptococci. Addition of purified cryptococcal polysaccharide to non-encapsulated cells inhibited phagocytosis of the yeast and induced a strong negative charge at the yeast surface. Chemical modification to reduce the surface charge of either purified cryptococcal polysaccharide or intact encapsulated cryptococci produced a small loss of phagocytosis-inhibiting activity; however, all treated polysaccharide preparations retained a significant ability to inhibit phagocytosis of the yeast. These results indicated that the association between surface charge and inhibition of phagocytosis was largely circumstantial, and presence of a negative surface charge could not account for the powerful antiphagocytic action of cryptococcal polysaccharide.

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