DEVELOPMENT OF A BIOMIMETIC ENZYME-LINKED IMMUNOSORBENT ASSAY METHOD BASED ON A HYDROPHILIC MOLECULARLY IMPRINTED POLYMER FILM FOR DETERMINATION OF OLAQUINDOX IN CHICK FEED SAMPLES

2013 ◽  
Vol 34 (1) ◽  
pp. 16-29 ◽  
Author(s):  
Dongyan Zhao ◽  
Xuguang Qiao ◽  
Zhixiang Xu ◽  
Rui Xu ◽  
Zhenhua Yan
Keyword(s):  
Polymer Film ◽  
Immunosorbent Assay ◽  
Molecularly Imprinted Polymer ◽  
Assay Method ◽  
Enzyme Linked Immunosorbent Assay ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Feed Samples

scholarly journals Development of a biomimetic enzyme-linked immunosorbent assay based on a molecularly imprinted polymer for the detection of cortisol in human saliva

2019 ◽  
Vol 11 (17) ◽  
pp. 2320-2326 ◽  
Author(s):  
Giulia Spano ◽  
Simone Cavalera ◽  
Fabio Di Nardo ◽  
Cristina Giovannoli ◽  
Laura Anfossi ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Molecularly Imprinted Polymer ◽  
Direct Determination ◽  
Human Saliva ◽  
Enzyme Linked Immunosorbent Assay ◽  
Imprinted Polymer ◽  
Molecularly Imprinted

A molecularly imprinted sorbent assays for cortisol was optimized for direct determination in human saliva.

Electrochemical sensor for determination of tulathromycin built with molecularly imprinted polymer film

2015 ◽  
Vol 407 (7) ◽  
pp. 1951-1959 ◽  
Author(s):  
Jiadi Sun ◽  
Jian Ji ◽  
Yifan Wang ◽  
Ying Zhao ◽  
Yinzhi Zhang ◽  
...  
Keyword(s):  
Electrochemical Sensor ◽  
Polymer Film ◽  
Molecularly Imprinted Polymer ◽  
Imprinted Polymer ◽  
Molecularly Imprinted

scholarly journals Determination of Asymmetric Dimethylarginine by Using Organic Semiconductor-Based Molecularly Imprinted Polymer Film

2018 ◽  
Vol 7 (7) ◽  
pp. Q3189-Q3195
Author(s):  
Valerii Malyshev ◽  
Agnieszka Michota-Kamińska ◽  
Shuai Shao ◽  
Francis D'Souza ◽  
Krzysztof Noworyta
Keyword(s):  
Polymer Film ◽  
Molecularly Imprinted Polymer ◽  
Organic Semiconductor ◽  
Asymmetric Dimethylarginine ◽  
Imprinted Polymer ◽  
Molecularly Imprinted

Molecularly imprinted polymer nanoparticle-based assay (MINA): application for fumonisin B1 determination

The Analyst ◽  
2018 ◽  
Vol 143 (14) ◽  
pp. 3481-3488 ◽  
Author(s):  
Hasim Munawar ◽  
Katarzyna Smolinska-Kempisty ◽  
Alvaro Garcia Cruz ◽  
Francesco Canfarotta ◽  
Elena Piletska ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Molecularly Imprinted Polymer ◽  
Animal Feed ◽  
Fumonisin B1 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Polymer Nanoparticle ◽  
Standard Tool

The enzyme-linked immunosorbent assay (ELISA) has been used as a standard tool for monitoring food and animal feed contamination from the carcinogenic fumonisin B1 (FB1).

Rapid Determination of Metolcarb Residues in Foods Using a Biomimetic Enzyme-Linked Immunosorbent Assay Employing a Novel Molecularly Imprinted Polymer Film as Artificial Antibody

2013 ◽  
Vol 96 (2) ◽  
pp. 453-458 ◽  
Author(s):  
Yiwei Tang ◽  
Guozhen Fang ◽  
Shuo Wang ◽  
Jingwei Sun ◽  
Kun Qian
Keyword(s):  
Molecularly Imprinted Polymer ◽  
Rapid Determination ◽  
Bulk Polymerization ◽  
Cross Reactivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Imprinted Polymer ◽  
Adsorption Ability ◽  
Molecularly Imprinted ◽  
Related Compounds

Abstract A novel and fast competitive direct biomimetic ELISA (cd-BELISA) was developed for determination of the N-methylcarbamate insecticide metolcarb based on a molecularly imprinted polymer (MIP) film as the antibody mimic. The MIP film was directly synthesized on the surface of a 96-well plate by bulk polymerization. The synthesized film was characterized, and the results showed that the imprinted film exhibited antibody-antigen-like binding properties and rapid adsorption ability, which was particularly useful for cd-BELISA development. The cd-BELISA conditions were optimized in detail. Under the optimum conditions, the sensitivity and LOD of the cd-BELISA were found to be 17 and 0.12 μg/L, respectively. Cross-reactivity demonstrated that the cd-BELISA had a higher selectivity for metolcarb than for structurally related compounds. The developed method was applied to the determination of metolcarb in spiked apple juice, cabbage, and cucumber, with mean recoveries ranging from 71.5 to 117.0%. Validation of the results was conducted by HPLC with good correlation (r2 > 0.9562) between data obtained using these two methods. Therefore, the developed cd-BELISA has potential for the rapid determination of metolcarb in foods.

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