Use of DNA barcoding in wildlife forensics: a study of sambar deer (Rusa unicolor)

2012 ◽  
Vol 8 (4) ◽  
pp. 224-226 ◽  
Author(s):  
U. Suresh Kumar ◽  
R.V. Ratheesh ◽  
G. Thomas ◽  
S. George
2021 ◽  
Vol 3 (2) ◽  
pp. 260-272
Author(s):  
Mukesh Thakar ◽  
Tina Sharma

Disorganized and chaotic collection of the Euphorbia plant species from the wild is one of the major reasons for its endangered status. According to CITES, the trade in Euphorbia royleana species is prohibited under Appendix II. However, the trade continues unabated as current identification methods do not discriminate between closely related species.  In the present study, a DNA barcoding method has been used to establish inter- and intra-specific divergences of both matK and rbcL regions by using pairwise genetic distance measurement methods for evaluating the maximum barcoding gap. The matk and rbcL yielded a 100% amplification and sequencing success rate to distinguish closely related species of Euphorbia royleana unambiguously. The matk and rbcL showed average interspecific genetic distance divergence values of 0.031and 0.015, respectively. The maximum number of species-specific SNPs was observed in matK sequences at seven consecutive sites, which could distinguish Euphorbia royleana from closely related species.  The best candidate barcoding region to identify Euphorbia royleana was found to be matK with a single-locus barcoding approach. Furthermore, the species discrimination method was developed with the help of species-specific SNPs derived from the matK barcoding region to accurately authenticate Euphorbia royleana, and it provided 100% species resolution


2018 ◽  
Vol 3 (1) ◽  
pp. 250-255 ◽  
Author(s):  
Ved P. Kumar ◽  
Malay Shukla ◽  
Ankita Rajpoot ◽  
Mukesh Thakur ◽  
Parag Nigam ◽  
...  

2013 ◽  
Vol 228 (1-3) ◽  
pp. 160-164 ◽  
Author(s):  
Eva A. Rolo ◽  
Ana Rita Oliveira ◽  
Catarina G. Dourado ◽  
Ana Farinha ◽  
Maria Teresa Rebelo ◽  
...  

Genome ◽  
2015 ◽  
Vol 58 (5) ◽  
pp. 151-162 ◽  
Author(s):  
Sarah J. Adamowicz

The 6th International Barcode of Life Conference (Guelph, Canada, 18–21 August 2015), themed Barcodes to Biomes, showcases the latest developments in DNA barcoding research and its diverse applications. The meeting also provides a venue for a global research community to share ideas and to initiate collaborations. All plenary and contributed abstracts are being published as an open-access special issue of Genome. Here, I use a comparison with the 3rd Conference (Mexico City, 2009) to highlight 10 recent and emerging trends that are apparent among the contributed abstracts. One of the outstanding trends is the rising proportion of abstracts that focus upon multiple socio-economically important applications of DNA barcoding, including studies of agricultural pests, quarantine and invasive species, wildlife forensics, disease vectors, biomonitoring of ecosystem health, and marketplace surveys evaluating the authenticity of seafood products and medicinal plants. Other key movements include the use of barcoding and metabarcoding approaches for dietary analyses—and for studies of food webs spanning three or more trophic levels—as well as the spread of next-generation sequencing methods in multiple contexts. In combination with the rising taxonomic and geographic scope of many barcoding iniatives, these developments suggest that several important questions in biology are becoming tractable. “What is this specimen on an agricultural shipment?”, “Who eats whom in this whole food web?”, and even “How many species are there?” are questions that may be answered in time periods ranging from a few years to one or a few decades. The next phases of DNA barcoding may expand yet further into prediction of community shifts with climate change and improved management of biological resources.


2017 ◽  
Vol 27 (4) ◽  
pp. 483-494 ◽  
Author(s):  
ANDREAS C. DIMITRIOU ◽  
GIOVANNI FORCINA ◽  
CLAIRIE PAPAZOGLOU ◽  
PANICOS PANAYIDES ◽  
MONICA GUERRINI ◽  
...  

SummaryDNA barcoding has become a widely used and valuable tool in taxonomic research during the past three decades and its application is expanding to fields such as wildlife forensics (e.g. anti-poaching actions). One of the major advantages of DNA barcoding is that it allows for species identification from largely incomplete, deformed or cooked samples and even from traces of biological material. Cyprus is a well-known biodiversity hotspot, the island avifauna comprising around 400 species, 117 of which are listed in Annex I of the Birds Directive (2009/147/EC). Each year on average, 2.3 million individuals (mainly small migratory birds) are trapped illegally on the island and often served at restaurants. Consequently, reliable identification of specimens from processed tissues can be crucial for undertaking legal actions. Herein DNA barcoding of the large majority of bird species resident in Cyprus plus several migrants that were illegally captured was carried out to support local authorities in their anti-poaching actions. In this study, tissue from 103 specimens representing 81 bird taxa were sampled and a 648-bp long fragment of the Cytochrome Oxidase subunit Ι (COI) gene was targeted using a combination of standard, nested and semi-nested PCR depending on the condition of the tissue at hand. Sequences were obtained from all investigated samples and a total of 92 haplotypes was recovered. Phylogenetic reconstructions returned a pattern of relationships among taxa largely compatible with known avian phylogeny. Furthermore, diagnostic polymorphisms within the genus including one of the main targeted species, Sylvia atricapilla, were found. In conclusion, an avian genetic dataset is now available for the law enforcement authorities to identify specimens either confiscated (even when morphologically deformed or cooked) during raids in restaurants and in other premises suspected of serving illegally protected bird species or from trappers.


Sign in / Sign up

Export Citation Format

Share Document