Shikimic Acid Production byFusarium decemcellulare, An Endophytic Fungus Isolated fromFlacourtia inermisFruits

Flux balance analysis (FBA) is used to improve the microbial production of useful compounds. However, a large gap often exists between the FBA solution and the experimental yield, because of growth and byproducts. FBA has been extended to dynamic FBA (dFBA), which is applicable to time-varying processes, such as batch or fed-batch cultures, and has significantly contributed to metabolic and cultural engineering applications. On the other hand, the performance of the experimental strains has not been fully evaluated. In this study, we applied dFBA to the production of shikimic acid from glucose in Escherichia coli, to evaluate the production performance of the strain as a case study. The experimental data of glucose consumption and cell growth were used as FBA constraints. Bi-level FBA optimization with maximized growth and shikimic acid production were the objective functions. Results suggest that the shikimic acid concentration in the high-shikimic-acid-producing strain constructed in the experiment reached up to 84% of the maximum value by simulation. Thus, this method can be used to evaluate the performance of strains and estimate the milestones of strain improvement.

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Shikimic acid production by a modified strain ofE. coli (W3110.shik1) under phosphate-limited and carbon-limited conditions

Biotechnology and Bioengineering ◽

10.1002/bit.20546 ◽

2005 ◽

Vol 92 (5) ◽

pp. 541-552 ◽

Cited By ~ 58

Author(s):

Louise Johansson ◽

Anna Lindskog ◽

Gustav Silfversparre ◽

Christian Cimander ◽

Kristian Fog Nielsen ◽

...

Keyword(s):

Acid Production ◽

Shikimic Acid ◽

Shikimic Acid Production

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Metabolic modeling and response surface analysis of an Escherichia coli strain engineered for shikimic acid production

BMC Systems Biology ◽

10.1186/s12918-018-0632-4 ◽

2018 ◽

Vol 12 (1) ◽

Cited By ~ 5

Author(s):

Juan A. Martínez ◽

Alberto Rodriguez ◽

Fabian Moreno ◽

Noemí Flores ◽

Alvaro R. Lara ◽

...

Keyword(s):

Escherichia Coli ◽

Response Surface ◽

Surface Analysis ◽

Acid Production ◽

Shikimic Acid ◽

Metabolic Modeling ◽

Coli Strain ◽

Response Surface Analysis ◽

Shikimic Acid Production

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Shikimic Acid Production from Ginkgo biloba via Callus Culture

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.931-932.1524 ◽

2014 ◽

Vol 931-932 ◽

pp. 1524-1528 ◽

Cited By ~ 1

Author(s):

Siriporn Phongtongpasuk ◽

Phitchayakon Piemthongkham

Keyword(s):

Plant Growth ◽

Callus Culture ◽

Growth Regulators ◽

Ginkgo Biloba ◽

Callus Induction ◽

Acid Content ◽

Acid Production ◽

Shikimic Acid ◽

Fresh Weight ◽

Shikimic Acid Production

Shikimic acid is a very important precursor for industrial synthesis of oseltamivir (Tamiflu®) which is used for the antiviral treatment. In this study, callus culture of Ginkgo biloba for shikimic acid production was reported. Callus induced from either leaves or nodal stems of sterilized ginkgo was grown on MS medium supplemented with a combination of plant growth regulators as followed: MS+KD, MS+BD, MS+KN and MS+BN for 90 days. Morphological changes, fresh weight and shikimic acid content of callus in each medium were monitored every 30 days. The result showed that callus cultures from each treatment were morphologically different. It is likely due to explant used for callus induction and type of plant growth regulators added into the medium. Browning effect was noticeably detected from 60 days to 90 days. Moreover, fresh weight and shikimic acid content of callus culture depended on cultivation time, cultivation medium and type of explants used for callus induction. Callus induced from nodal stem grown on MS+BN for 30 days offered the highest fresh weight. For shikimic acid production, the most satisfied quantity of shikimic acid was achieved from callus cultured on MS+KN for 30 days by exploiting nodal stem as explant.

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