scholarly journals The status of Bactrocera invadens Drew, Tsuruta & White (Diptera: tephritidae) inferred from complete mitochondrial genome analysis

2016 ◽  
Vol 1 (1) ◽  
pp. 680-681 ◽  
Author(s):  
Li-Jie Zhang ◽  
Li-Hui Jiang ◽  
Chun-Yan Wei ◽  
Ruo-Si Liu ◽  
Xing-Liang Liu ◽  
...  
Keyword(s):  
Mitochondrial Genome ◽  
Genome Analysis ◽  
Complete Mitochondrial Genome ◽  
The Status ◽  
Bactrocera Invadens

Complete mitochondrial genome analysis of Clinostomum complanatum and its comparison with selected digeneans

2016 ◽  
Vol 115 (8) ◽  
pp. 3249-3256 ◽  
Author(s):  
Lu Chen ◽  
Yan Feng ◽  
Hong-Mei Chen ◽  
Li-Xia Wang ◽  
Han-Li Feng ◽  
...  
Keyword(s):  
Mitochondrial Genome ◽  
Genome Analysis ◽  
Complete Mitochondrial Genome ◽  
Clinostomum Complanatum

scholarly journals A Protocol for mtGenome Analysis on Large Sample Numbers

2014 ◽  
Vol 8 ◽  
pp. BBI.S14623 ◽  
Author(s):  
Igor G. Hamoy ◽  
André M. Ribeiro-Dos-Santos ◽  
Luiz Alvarez ◽  
Silvanira Barbosa ◽  
Artur Silva ◽  
...  
Keyword(s):  
Mitochondrial Genome ◽  
High Throughput ◽  
Genome Analysis ◽  
High Throughput Sequencing ◽  
Complete Mitochondrial Genome ◽  
High Quality ◽  
Sequencing Technologies ◽  
Ion Pgm ◽  
Total Variability ◽  
Run Up

The mitochondrial genome is widely studied in a variety of fields, such as population, forensic, and human and medical genetics. Most studies have been limited to a small portion of the sequence that, although highly diverse, does not describe the total variability. The arrival of modern high-throughput sequencing technologies has made it possible to investigate larger sequences in a shorter amount of time as well as in a more affordable fashion. This work aims to describe a protocol for sequencing and analyzing the complete mitochondrial genome with the Ion PGM™ platform. To evaluate the protocol, the mitochondrial genome was sequenced to approximately 210 Mbp, with high-quality sequences distributed between 12 samples that had an average coverage of 1023× per sample. Several variant callers were compared to improve the protocol outcome. The results suggest that it is possible to run up to 120 samples per run without any loss of any significant quality. Therefore, this protocol is an efficient and accurate tool for full mitochondrial genome analysis.

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