Evaluation of Methods for Determination of Soil Boron Available to Apple Trees

2005 ◽  
Vol 36 (4-6) ◽  
pp. 695-700 ◽  
Author(s):  
Christos D. Tsadilas ◽  
Panagiota Kazai
Author(s):  
Robert Skov ◽  
David R Lonsway ◽  
Jesper Larsen ◽  
Anders Rhod Larsen ◽  
Jurgita Samulioniené ◽  
...  

Abstract Objectives Correct determination of penicillin susceptibility is pivotal for using penicillin in the treatment of Staphylococcus aureus infections. This study examines the performance of MIC determination, disc diffusion and a range of confirmatory tests for detection of penicillin susceptibility in S. aureus. Methods A total of 286 consecutive penicillin-susceptible S. aureus blood culture isolates as well as a challenge set of 62 MSSA isolates were investigated for the presence of the blaZ gene by PCR and subjected to penicillin-susceptibility testing using broth microdilution MIC determination, disc diffusion including reading of the zone edge, two nitrocefin tests and the cloverleaf test. Results Using PCR-based detection of blaZ as the gold standard, both broth microdilution MIC testing and disc diffusion testing resulted in a relatively low accuracy (82%–93%) with a sensitivity ranging from 49%–93%. Among the confirmatory tests, the cloverleaf test performed with 100% accuracy, while zone edge interpretation and nitrocefin-based tests increased the sensitivity of β-lactamase detection to 96%–98% and 82%–96% when using MIC determination or disc diffusion as primary test, respectively. Conclusions This investigation showed that reliable and accurate detection of β-lactamase production in S. aureus can be obtained by MIC determination or penicillin disc diffusion followed by interpretation of the zone edge as a confirmatory test for apparently penicillin-susceptible isolates. The more cumbersome cloverleaf test can also be used. Nitrocefin-based tests should not be used as the only test for confirmation of a presumptive β-lactamase-negative isolate.


2005 ◽  
Vol 27 (11) ◽  
pp. 2007-2018 ◽  
Author(s):  
Sahriye Sönmez ◽  
Mustafa Kaplan

2003 ◽  
Vol 16 (8) ◽  
pp. 1126-1130
Author(s):  
J. Sekine ◽  
Hossam E. M. Kamel ◽  
Abdel Nasir M. A. Fadel El-Seed ◽  
M. Hishinuma

1993 ◽  
pp. 37-41 ◽  
Author(s):  
O. Failla ◽  
G. Stringari ◽  
D. Porro ◽  
A. Scienza
Keyword(s):  

2007 ◽  
Vol 39 (1) ◽  
pp. 31-40 ◽  
Author(s):  
A. Körner ◽  
G. Gelbrich ◽  
G. Müller ◽  
A. Reich ◽  
K. Deutscher ◽  
...  

1989 ◽  
Vol 72 (2) ◽  
pp. 360-366 ◽  
Author(s):  
G.F. Senyk ◽  
J.A. Scheib ◽  
J.M. Brown ◽  
R.A. Ledford

Pramana ◽  
1989 ◽  
Vol 32 (2) ◽  
pp. 143-148 ◽  
Author(s):  
T S Krishnamoorthy ◽  
N Mahadevan ◽  
S S Desai ◽  
C C Dias

2000 ◽  
Vol 109 (2) ◽  
pp. 167-179 ◽  
Author(s):  
Søren Husted ◽  
Christopher A. Hebbern ◽  
Marie Mattsson ◽  
Jan K. Schjoerring

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