SIMULTANEOUS DETERMINATION OF INORGANIC ANIONS AND METHYL AND ETHYLPHOSPHONIC ACIDS BY ANION EXCHANGE CHROMATOGRAPHY USING EVAPORATIVE LIGHT SCATTERING DETECTION

2000 ◽  
Vol 23 (15) ◽  
pp. 2345-2359 ◽  
Author(s):  
Jean -Philippe Mercier ◽  
Claire Elfakir ◽  
Michel Dreux ◽  
Saïd Lazar ◽  
Mohammadine El Haddad ◽  
...  
Keyword(s):  
Light Scattering ◽  
Simultaneous Determination ◽  
Anion Exchange ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Inorganic Anions ◽  
Evaporative Light Scattering Detection ◽  
Light Scattering Detection ◽  
Evaporative Light Scattering

scholarly journals Simultaneous Determination of Four Active Ingredients inSargentodoxa cuneataby HPLC Coupled with Evaporative Light Scattering Detection

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Di-Hua Li ◽  
Yuan-Shan Lv ◽  
Jun-Hong Liu ◽  
Lei Yang ◽  
Yan Wang ◽  
...  
Keyword(s):  
Light Scattering ◽  
Simultaneous Determination ◽  
Flow Rate ◽  
Evaporative Light Scattering Detection ◽  
Light Scattering Detection ◽  
Evaporative Light Scattering ◽  
Relative Standard ◽  
Acid Aqueous Solution ◽  
Interday Precision

A HPLC coupled with evaporative light scattering detection method had been developed for the simultaneous determination of 3,4-dihydroxyphenylethyl alcohol glycoside, salidroside, chlorogenic acid, and liriodendrin in the stem ofSargentodoxa cuneata. With a C18 column, the analysis was performed using acetonitrile and 0.2% formic acid aqueous solution as mobile phase in gradient program at a flow rate of 0.9 mL/min. The optimum drift tube temperature of evaporative light scattering detection was at 105°C with the air flow rate of 2.5 L/min. The calibration curves showed good linearity during the test ranges. This method was validated for limits of detection and quantification, precision, and reproducibility. The recoveries were within the range of 96.39%–104.64%. The relative standard deviations of intraday and interday precision were less than 2.90% and 3.30%, respectively. The developed method can be successfully used to quantify the four analytes in the stem ofSargentodoxa cuneatafrom various regions in China.

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