scholarly journals Inheritance of yeast nuclear pore complexes requires the Nsp1p subcomplex

2013 ◽  
Vol 203 (2) ◽  
pp. 187-196 ◽  
Author(s):  
Tadashi Makio ◽  
Diego L. Lapetina ◽  
Richard W. Wozniak
Keyword(s):  
Daughter Cell ◽  
Selection Process ◽  
Nuclear Pore ◽  
Nuclear Pore Complexes ◽  
Yeast Saccharomyces Cerevisiae ◽  
Macromolecular Complexes ◽  
Daughter Cells ◽  
Bud Neck ◽  
Pore Complexes ◽  
Mother Nucleus

In the yeast Saccharomyces cerevisiae, organelles and macromolecular complexes are delivered from the mother to the emerging daughter during cell division, thereby ensuring progeny viability. Here, we have shown that during mitosis nuclear pore complexes (NPCs) in the mother nucleus are actively delivered through the bud neck and into the daughter cell concomitantly with the nuclear envelope. Furthermore, we show that NPC movement into the daughter cell requires members of an NPC subcomplex containing Nsp1p and its interacting partners. NPCs lacking these nucleoporins (Nups) were blocked from entry into the daughter by a putative barrier at the bud neck. This selection process could be observed within individual cells such that NPCs containing Nup82p (an Nsp1p-interacting Nup) were transferred to the daughter cells while functionally compromised NPCs lacking Nup82p were retained in the mother. This mechanism is proposed to facilitate the inheritance of functional NPCs by daughter cells.

scholarly journals Ensuring that yeast cells get their inheritance

2013 ◽  
Vol 203 (2) ◽  
pp. 167-167
Author(s):  
Mitch Leslie
Keyword(s):  
Daughter Cell ◽  
Yeast Cells ◽  
Nuclear Pore ◽  
Nuclear Pore Complexes ◽  
Pore Complexes

A nucleoporin allows nuclear pore complexes access to daughter cell during mitosis.

scholarly journals Nuclear Pore Complex Acetylation Regulates mRNA Export and Cell Cycle Commitment in Budding Yeast

2021 ◽  
Author(s):  
Mercè Gomar-Alba ◽  
Vasilisa Pozharskaia ◽  
Celia Schaal ◽  
Arun Kumar ◽  
Basile Jacquel ◽  
...  
Keyword(s):  
Gene Expression ◽  
Nuclear Export ◽  
Mrna Export ◽  
Cell Types ◽  
Nuclear Pore ◽  
Specific Cell ◽  
Nuclear Pore Complexes ◽  
Daughter Cells ◽  
Associated Proteins ◽  
Pore Complexes

AbstractNuclear pore complexes (NPCs) mediate communication between the nucleus and the cytoplasm and regulate gene expression by interacting with transcription and mRNA export factors. Lysine acetyl-transferases (KATs) promote transcription through acetylation of chromatin-associated proteins. We find that Esa1, the KAT subunit of the yeast NuA4 complex, also acetylates the nuclear pore basket component Nup60 to promote mRNA export. Acetylation of Nup60 recruits mRNA export factors to the nuclear basket, including the scaffolding subunit of the Transcription and Export 2 (TREX-2) complex, Sac3. Esa1-dependent nuclear export of mRNAs promotes entry into S phase, and is inhibited by the Hos3 deacetylase in G1 daughter cells to restrain their premature commitment to a new cell division cycle. This mechanism also inhibits expression of the nutrient-regulated GAL1 gene specifically in daughter cells. These results reveal how acetylation contributes to the functional plasticity of NPCs in specific cell types, and demonstrate how the evolutionarily conserved NuA4 complex regulates gene expression dually at the level of transcription and mRNA export, by modifying the nucleoplasmic entrance to nuclear pores.

scholarly journals Daughter-cell-specific modulation of nuclear pore complexes controls cell cycle entry during asymmetric division

2018 ◽  
Vol 20 (4) ◽  
pp. 432-442 ◽  
Author(s):  
Arun Kumar ◽  
Priyanka Sharma ◽  
Mercè Gomar-Alba ◽  
Zhanna Shcheprova ◽  
Anne Daulny ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Daughter Cell ◽  
Asymmetric Division ◽  
Nuclear Pore ◽  
Nuclear Pore Complexes ◽  
Cell Cycle Entry ◽  
Pore Complexes

Nucleocytoplasmic transport of macromolecules

1997 ◽  
Vol 61 (2) ◽  
pp. 193-211
Author(s):  
A H Corbett ◽  
P A Silver
Keyword(s):  
Nuclear Envelope ◽  
Nuclear Transport ◽  
Bound State ◽  
Nucleocytoplasmic Transport ◽  
Nuclear Pore ◽  
Nuclear Pore Complexes ◽  
Yeast Saccharomyces Cerevisiae ◽  
Soluble Factors ◽  
Pore Complexes ◽  
Transport Of Macromolecules

Nucleocytoplasmic transport is a complex process that consists of the movement of numerous macromolecules back and forth across the nuclear envelope. All macromolecules that move in and out of the nucleus do so via nuclear pore complexes that form large proteinaceous channels in the nuclear envelope. In addition to nuclear pores, nuclear transport of macromolecules requires a number of soluble factors that are found both in the cytoplasm and in the nucleus. A combination of biochemical, genetic, and cell biological approaches have been used to identify and characterize the various components of the nuclear transport machinery. Recent studies have shown that both import to and export from the nucleus are mediated by signals found within the transport substrates. Several studies have demonstrated that these signals are recognized by soluble factors that target these substrates to the nuclear pore. Once substrates have been directed to the pore, most transport events depend on a cycle of GTP hydrolysis mediated by the small Ras-like GTPase, Ran, as well as other proteins that regulate the guanine nucleotide-bound state of Ran. Many of the essential factors have been identified, and the challenge that remains is to determine the exact mechanism by which transport occurs. This review attempts to present an integrated view of our current understanding of nuclear transport while highlighting the contributions that have been made through studies with genetic organisms such as the budding yeast, Saccharomyces cerevisiae.

scholarly journals TORC1 inactivation stimulates autophagy of nucleoporin and nuclear pore complexes

2020 ◽  
Vol 219 (7) ◽  
Author(s):  
Yui Tomioka ◽  
Tetsuya Kotani ◽  
Hiromi Kirisako ◽  
Yu Oikawa ◽  
Yayoi Kimura ◽  
...  
Keyword(s):  
Membrane Vesicles ◽  
Specific Factor ◽  
Nuclear Pore ◽  
Nuclear Pore Complexes ◽  
Binding Ability ◽  
Yeast Saccharomyces Cerevisiae ◽  
Selective Autophagy ◽  
Tor Kinase ◽  
Autophagic Degradation ◽  
Pore Complexes

The mechanisms underlying turnover of the nuclear pore complex (NPC) and the component nucleoporins (Nups) are still poorly understood. In this study, we found that the budding yeast Saccharomyces cerevisiae triggers NPC degradation by autophagy upon the inactivation of Tor kinase complex 1. This degradation largely depends on the selective autophagy-specific factor Atg11 and the autophagy receptor–binding ability of Atg8, suggesting that the NPC is degraded via receptor-dependent selective autophagy. Immunoelectron microscopy revealed that NPCs embedded in nuclear envelope–derived double-membrane vesicles are sequestered within autophagosomes. At least two pathways are involved in NPC degradation: Atg39-dependent nucleophagy (selective autophagy of the nucleus) and a pathway involving an unknown receptor. In addition, we found the interaction between Nup159 and Atg8 via the Atg8-family interacting motif is important for degradation of this nucleoporin not assembled into the NPC. Thus, this study provides the first evidence for autophagic degradation of the NPC and Nups, which we term “NPC-phagy” and “nucleoporinophagy.”

scholarly journals The transmission of nuclear pore complexes to daughter cells requires a cytoplasmic pool of Nsp1

2013 ◽  
Vol 203 (2) ◽  
pp. 215-232 ◽  
Author(s):  
Paolo Colombi ◽  
Brant M. Webster ◽  
Florian Fröhlich ◽  
C. Patrick Lusk
Keyword(s):  
De Novo ◽  
Selective Inhibition ◽  
Nuclear Pore ◽  
Nuclear Pore Complexes ◽  
Protein Assemblies ◽  
Class V ◽  
Specific Loss ◽  
Daughter Cells ◽  
Mother And Daughter ◽  
Pore Complexes

Nuclear pore complexes (NPCs) are essential protein assemblies that span the nuclear envelope and establish nuclear–cytoplasmic compartmentalization. We have investigated mechanisms that control NPC number in mother and daughter cells during the asymmetric division of budding yeast. By simultaneously tracking existing NPCs and newly synthesized NPC protomers (nups) through anaphase, we uncovered a pool of the central channel nup Nsp1 that is actively targeted to the bud in association with endoplasmic reticulum. Bud targeting required an intact actin cytoskeleton and the class V myosin, Myo2. Selective inhibition of cytoplasmic Nsp1 or inactivation of Myo2 reduced the inheritance of NPCs in daughter cells, leading to a daughter-specific loss of viability. Our data are consistent with a model in which Nsp1 releases a barrier that otherwise prevents NPC passage through the bud neck. It further supports the finding that NPC inheritance, not de novo NPC assembly, is primarily responsible for controlling NPC number in daughter cells.

scholarly journals Cell-specific modulation of nuclear pore complexes controls cell cycle entry during asymmetric division

2017 ◽  
Author(s):  
Arun Kumar ◽  
Priyanka Sharma ◽  
Zhanna Shcheprova ◽  
Anne Daulny ◽  
Trinidad Sanmartín ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Fate ◽  
Daughter Cell ◽  
Asymmetric Division ◽  
Nuclear Accumulation ◽  
Nuclear Pore ◽  
Nuclear Pore Complexes ◽  
Cell Cycle Entry ◽  
Independent Functions ◽  
Pore Complexes

SummaryThe acquisition of cellular identity is coupled to changes in the nuclear periphery and nuclear pore complexes (NPCs). Whether and how these changes determine cell fate remains unclear. We have uncovered a mechanism regulating NPC acetylation to direct cell fate after asymmetric division in budding yeast. The lysine deacetylase Hos3 associates specifically with daughter cell NPCs during mitosis to delay cell cycle entry (Start). Hos3-dependent deacetylation of nuclear basket and central channel nucleoporins establishes daughter cell-specific nuclear accumulation of the transcriptional repressor Whi5 during anaphase and perinuclear silencing of the CLN2 gene in the following G1 phase. Hos3-dependent coordination of both events restrains Start in daughter but not in mother cells. We propose that deacetylation modulates transport-dependent and - independent functions of NPCs, leading to differential cell cycle progression in mother and daughter cells. Similar mechanisms might regulate NPC functions in specific cell types and/or cell cycle stages in multicellular organisms.

scholarly journals Defects in nuclear pore assembly lead to activation of an Aurora B–mediated abscission checkpoint

2010 ◽  
Vol 191 (5) ◽  
pp. 923-931 ◽  
Author(s):  
Douglas R. Mackay ◽  
Masaki Makise ◽  
Katharine S. Ullman
Keyword(s):  
Small Interfering Rna ◽  
Nuclear Pore ◽  
Aurora B ◽  
Nuclear Pore Complexes ◽  
Daughter Cells ◽  
Nuclear Environment ◽  
Genomic Regulation ◽  
Pore Complexes ◽  
Interfering Rna ◽  
Nuclear Pore Assembly

Correct assembly of nuclear pore complexes (NPCs), which directly and indirectly control nuclear environment and architecture, is vital to genomic regulation. We previously found that nucleoporin 153 (Nup153) is required for timely progression through late mitosis. In this study, we report that disruption of Nup153 function by either small interfering RNA–mediated depletion or expression of a dominant-interfering Nup153 fragment results in dramatic mistargeting of the pore basket components Tpr and Nup50 in midbody-stage cells. We find a concomitant appearance of aberrantly localized active Aurora B and an Aurora B–dependent delay in abscission. Depletion of Nup50 is also sufficient to increase the number of midbody-stage cells and, likewise, triggers distinctive mislocalization of Aurora B. Together, our results suggest that defects in nuclear pore assembly, and specifically the basket structure, at this time of the cell cycle activate an Aurora B–mediated abscission checkpoint, thereby ensuring that daughter cells are generated only when fully formed NPCs are present.

scholarly journals The dynamic three-dimensional organization of the diploid yeast genome

eLife ◽  
2017 ◽  
Vol 6 ◽  
Author(s):  
Seungsoo Kim ◽  
Ivan Liachko ◽  
Donna G Brickner ◽  
Kate Cook ◽  
William S Noble ◽  
...  
Keyword(s):  
Nuclear Periphery ◽  
Three Dimensional ◽  
Culture Conditions ◽  
Yeast Genome ◽  
Nuclear Pore ◽  
Nuclear Pore Complexes ◽  
Yeast Saccharomyces Cerevisiae ◽  
Chromosome Conformation ◽  
Homolog Pairing ◽  
Pore Complexes

The budding yeast Saccharomyces cerevisiae is a long-standing model for the three-dimensional organization of eukaryotic genomes. However, even in this well-studied model, it is unclear how homolog pairing in diploids or environmental conditions influence overall genome organization. Here, we performed high-throughput chromosome conformation capture on diverged Saccharomyces hybrid diploids to obtain the first global view of chromosome conformation in diploid yeasts. After controlling for the Rabl-like orientation using a polymer model, we observe significant homolog proximity that increases in saturated culture conditions. Surprisingly, we observe a localized increase in homologous interactions between the HAS1-TDA1 alleles specifically under galactose induction and saturated growth. This pairing is accompanied by relocalization to the nuclear periphery and requires Nup2, suggesting a role for nuclear pore complexes. Together, these results reveal that the diploid yeast genome has a dynamic and complex 3D organization.

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