scholarly journals Intracellular Distribution of 5' Nucleotidase in Rat Liver

1958 ◽  
Vol 4 (4) ◽  
pp. 373-376 ◽  
Author(s):  
Gaston de Lamirande ◽  
Claude Allard ◽  
Antonio Cantero
Keyword(s):  
Enzymatic Activity ◽  
Rat Liver ◽  
Biochemical Analysis ◽  
Inorganic Phosphorus ◽  
Intracellular Distribution ◽  
Differential Centrifugation ◽  
Nucleotidase Activity ◽  
Rat Liver Cells ◽  
Cell Fractions

The intracellular distribution of 5' nucleotidase was investigated in rat liver by biochemical analysis of cell fractions obtained by differential centrifugation. The enzymatic activity was measured by determination of the inorganic phosphorus liberated from 5' nucleotides. The 5' nucleotidase activity was mainly found in the nuclear and microsomal fractions. An attempt to extract the enzyme from these fractions with Mg++ ion solutions was unsuccessful. It is concluded that 5' nucleotidase is actually present in the nuclear and microsomal fractions of rat liver cells.

INTRACELLULAR DISTRIBUTION OF DESOXYRIBONUCLEO-DEPOLYMERASE IN NORMAL RAT LIVER, LIVER TUMOR, AND LIVER OF ANIMALS FED p-DIMETHYLAMINOAZOBENZENE

1954 ◽  
Vol 32 (1) ◽  
pp. 35-40 ◽  
Author(s):  
Gaston de Lamirande ◽  
Claude Allard ◽  
Antonio Cantero
Keyword(s):  
Rat Liver ◽  
Liver Tumor ◽  
Cell Activity ◽  
Mitotic Rate ◽  
Intracellular Distribution ◽  
Nuclear Fraction ◽  
Normal Rat ◽  
High Level ◽  
Soluble Material

The intracellular distribution of desoxyribonucleodepolymerase (DNase) has been investigated in the liver of animals fed p-dimethylaminoazobenzene (DAB), in liver freed from tumor, and in DAB induced tumor. The method is based on the determination of acid soluble material containing phosphorus, liberated by the action of the enzyme upon highly polymerized DNA. Results indicated that the nuclear DNase particularly accounts for a very low percentage of the whole cell activity in normal rat liver, whereas in nuclei of liver of DAB fed rats and of tumor the activity is increased to a high level. These facts suggest a possible correlation between the activity of DNase in the nuclear fraction and the mitotic rate of the tissue.

Antioxidant Constituents from the Stem and Leaf of Helicteres hirsuta Loureiro

2020 ◽  
Vol 10 ◽  
Author(s):  
Nguyen Thanh Tra ◽  
Ba Thi Cham ◽  
Nguyen Thi Thu Ha ◽  
Le Thi Tu Anh ◽  
Nguyen Van Tuyen ◽  
...  
Keyword(s):  
Rat Liver ◽  
Chemical Constituents ◽  
Positive Control ◽  
Liver Cells ◽  
Phytochemical Analysis ◽  
Etoac Extract ◽  
Mtt Method ◽  
Rat Liver Cells ◽  
First Time

Background: Helicteres hirsuta has been used traditionally as a useful agent for hepatoprotective treatment. The aim of the current study is to isolate chemical constituents from the EtOAc extract of Helicteres hirsuta stem and evaluate the capacity of the isolated compounds against hydroperoxide damaged rat liver cells Methods: Column chromatography was used for phytochemical isolation whereas MTT method was applied for intracellular antioxidative assay Results: Phytochemical analysis of the EtOAc extract of Helicteres hirsuta stem led to the isolation and determination of four triterpenoids betulin (1), bentulinic acid (2), alphitolic acid (3), and oleanolic acid (4), together with two steroids stigmast-4-ene-6β-ol-3-one (5), and β-sitostenone (6), whereas EtOAc extract of its leaf composed of three steroids cucurbitacin D (11), cucurbitacin I (12), and simiarenol (13), four flavonoids tiliroside (14), potengriffioside A (15), kaempferide (16), and isokaempferide (17), along with two carotenoids lutelin (18), and β-carotene (19). Isolated compounds 3, 5, 6, 16, and 17 were found in genus Helicteres for the first time, while carotenoids 18, and 19 were never isolated from family Sterculiaceae before. Phytochemicals derived H. hirsuta species are also useful agents for antioxidative drugs, e.g, flavonol 17 induced the significant EC50 value of 22.24 ± 0.14 μg/mL, as compared with that of positive control curcumin (EC50 19.33 ± 0.77 μg/mL), against hydroperoxide damaged rat liver cells Conclusion: Antioxidative activity of the EtOAc extract of Helicteres hirsuta stem against hydroperoxide is mostly based on the role of flavonoids.

Determination of glucuronidation in isolated rat liver cells by incorporation of 14C from fructose

1990 ◽  
Vol 18 (6) ◽  
pp. 1205-1205
Author(s):  
JOHN DAWSON ◽  
RICHARD G. KNOWLES ◽  
CHRISTOPHER I. POGSON
Keyword(s):  
Rat Liver ◽  
Liver Cells ◽  
Isolated Rat Liver ◽  
Rat Liver Cells ◽  
Isolated Rat

INTRACELLULAR DISTRIBUTION OF DESOXYRIBONUCLEO-DEPOLYMERASE IN NORMAL RAT LIVER, LIVER TUMOR, AND LIVER OF ANIMALS FED p-DIMETHYLAMINOAZOBENZENE

1954 ◽  
Vol 32 (1) ◽  
pp. 35-40 ◽  
Author(s):  
Gaston de Lamirande ◽  
Claude Allard ◽  
Antonio Cantero
Keyword(s):  
Rat Liver ◽  
Liver Tumor ◽  
Cell Activity ◽  
Mitotic Rate ◽  
Intracellular Distribution ◽  
Nuclear Fraction ◽  
Normal Rat ◽  
High Level ◽  
Soluble Material

The intracellular distribution of desoxyribonucleodepolymerase (DNase) has been investigated in the liver of animals fed p-dimethylaminoazobenzene (DAB), in liver freed from tumor, and in DAB induced tumor. The method is based on the determination of acid soluble material containing phosphorus, liberated by the action of the enzyme upon highly polymerized DNA. Results indicated that the nuclear DNase particularly accounts for a very low percentage of the whole cell activity in normal rat liver, whereas in nuclei of liver of DAB fed rats and of tumor the activity is increased to a high level. These facts suggest a possible correlation between the activity of DNase in the nuclear fraction and the mitotic rate of the tissue.

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